Ecology of the vagina upon Chlamydial infections

与人类阴道相关的微生物区系与人类宿主之间存在着相互关联的关系 并被认为对女性的生殖健康起着重要作用。阴道微生物群落 构成了抵御入侵的非本土生物感染的第一道防线，这些入侵生物会导致 疾病，如性传播的沙眼衣原体。 尽管它们很重要，但令人惊讶的是，利特菲知道阴道微生物的组成和动态 在健康和疾病方面交流。传统的基于邪教的方法提供了有价值的 人类阴道微生物区系的不完整图景。在这项研究中，我们将大规模-平行相结合 16S rRNA基因序列非培养非依赖分析测序技术研究 沙眼衣原体感染和沙眼衣原体感染患者阴道微生物区系的种类组成和丰度。 FRAC/70/r7af/S阳性的盆腔炎(PID)妇女。此外，我们还将建立 社区动态：一群妇女在一年或更长时间后进行纵向抽样 治疗。在这些女性中，我们将使用社区转录组学来确定这套基因 由阴道微生物群落表达。这些合并的数据将提供阴道的独特视图 衣原体感染期间和感染后的微生物区系动态(即代谢途径的详细图像 (直接或间接)被触发)，并将进一步发展我们的衣原体模型 感染和再感染。由于使用人类作为研究对象的局限性，使用了豚鼠 作为衣原体感染的动物模型。同时，我们将描述健康人阴道微生物区系的特征。 随着时间的推移，感染卡瓦线虫的雌性豚鼠。理解并描述 阴道微生物区系将有助于开发基于rafionale和rafionale的新方法 科学合理的原则，在治疗的同时操纵阴道微生物区系。 从这些生物样本中分离出的200多株沙眼衣原体或豚鼠衣原体的基因组将被 用454焦磷酸测序进行测序。这些序列将代表无与伦比的资源，将是 与研究团体分享。序列数据将与阴道进行相关分析 微生物区系和该联盟的两个项目所表征的表型。

The microbiota associated with the human vagina exists in a mutualisfic relafionship with the human host and is believed to play an important role in women's reproducfive health. The vaginal microbial communities constitute the first line of defense against infection by invasive non-indigenous organisms that cause disease, such as the sexually transmitted Chlamydia trachomatis. Despite their importance, surprisingly litfie is known about the composition and dynamics of vaginal microbial communifies in health and disease. Traditional culfivafion-based methods have provided a valuable but incomplete picture of the human vaginal microbiota. In this study, we will combined massively-parallel sequencing technology with the culture-independent analysis of the 16S rRNA gene sequence to survey the vaginal microbiota species composifion and abundance in young adults with C. trachomatis infecfion and C. frac/70/r7af/s-positive women with pelvic inflammatory disease (PID). In addifion, we will establish the dynamics of the community a subgroup of women sampled longitudinally over one year or more after treatment. In each of these women, we will use community transcriptomics to identify the suite of genes expressed by the vaginal microbial community. This combined data will afford a unique view of the vaginal microbiota dynamics during and after Chlamydial infecfion (i.e., a detailed picture of the metabolic pathways triggered in response to the infecfions (direcfiy or indirectly)], and will further our model of Chlamydial infecfion and re-ihfecfion. Because of limitations in using humans as research subjects, guinea pigs are used as animal model for Chlamydial infections. Similarlly, we will characterize the vaginal microbiota in healthy and C. cawae-infected female guinea pigs over time. Understand and characterizing the importance of the vaginal microbiota will contribute greafiy to the development of new approaches based on rafionale and scienfifically sound principles to manipulate the vaginal microbiota in parallel to treatments. The genome of more than 200 C.trachomatis or C. caviae isolated from these biological samples will be sequenced using 454 pyrosequencing. These sequences will represent an unparalleled resource that will be shared with the research community. The sequence data will be analyzed in correlafion with the vaginal microbiota and the phenotypes characterized under the two projects of this consortium.