Ecology of the vagina upon Chlamydial infections
衣原体感染时阴道的生态学
基本信息
- 批准号:7762439
- 负责人:
- 金额:$ 40.51万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-09-21 至 2014-08-31
- 项目状态:已结题
- 来源:
- 关键词:African AmericanAftercareAgeAnimal ModelArtsBiologicalCaucasiansCaucasoid RaceCaviaChlamydia InfectionsChlamydia trachomatisClinicalCommunitiesDataDevelopmentDiagnosisDiseaseEcologyEcosystemEnvironmentFemaleFemale AdolescentsGenesGenital systemGenomeGenomicsGoalsHealthHumanIndigenousInfectionInfertilityLaboratoriesLeadMetabolic PathwayMethodsMicrobeModelingNorth AmericaOrganismPelvic Inflammatory DiseasePhenotypePlayPredispositionProcessReproductive HealthReproductive Tract InfectionsResearchResearch SubjectsResourcesRiskRoleSamplingStructureSubgroupSurveysTechnologyTestingTimeVaginaWomanbaseinsightmicrobialmicrobial communitynovel strategiespathogenrRNA Genesreproductiveresilienceresponsesexually activesoundtranscriptomicsyoung adult
项目摘要
The microbiota associated with the human vagina exists in a mutualisfic relafionship with the human host
and is believed to play an important role in women's reproducfive health. The vaginal microbial communities
constitute the first line of defense against infection by invasive non-indigenous organisms that cause
disease, such as the sexually transmitted Chlamydia trachomatis.
Despite their importance, surprisingly litfie is known about the composition and dynamics of vaginal microbial
communifies in health and disease. Traditional culfivafion-based methods have provided a valuable but
incomplete picture of the human vaginal microbiota. In this study, we will combined massively-parallel
sequencing technology with the culture-independent analysis of the 16S rRNA gene sequence to survey the
vaginal microbiota species composifion and abundance in young adults with C. trachomatis infecfion and C.
frac/70/r7af/s-positive women with pelvic inflammatory disease (PID). In addifion, we will establish the
dynamics of the community a subgroup of women sampled longitudinally over one year or more after
treatment. In each of these women, we will use community transcriptomics to identify the suite of genes
expressed by the vaginal microbial community. This combined data will afford a unique view of the vaginal
microbiota dynamics during and after Chlamydial infecfion (i.e., a detailed picture of the metabolic pathways
triggered in response to the infecfions (direcfiy or indirectly)], and will further our model of Chlamydial
infecfion and re-ihfecfion. Because of limitations in using humans as research subjects, guinea pigs are used
as animal model for Chlamydial infections. Similarlly, we will characterize the vaginal microbiota in healthy
and C. cawae-infected female guinea pigs over time. Understand and characterizing the importance of the
vaginal microbiota will contribute greafiy to the development of new approaches based on rafionale and
scienfifically sound principles to manipulate the vaginal microbiota in parallel to treatments.
The genome of more than 200 C.trachomatis or C. caviae isolated from these biological samples will be
sequenced using 454 pyrosequencing. These sequences will represent an unparalleled resource that will be
shared with the research community. The sequence data will be analyzed in correlafion with the vaginal
microbiota and the phenotypes characterized under the two projects of this consortium.
与人类阴道相关的微生物群与人类宿主存在互利关系
并被认为对妇女的生殖健康起着重要作用。阴道微生物群落
构成了抵御入侵性非本土生物感染的第一道防线,
性传播疾病,如沙眼衣原体。
尽管它们的重要性,令人惊讶的是,litfie知道阴道微生物的组成和动力学,
在健康和疾病中传播。传统的基于培养的方法提供了一个有价值的,但
人类阴道微生物群的不完整图片。在本研究中,我们将结合
测序技术与16 S rRNA基因序列的非培养分析,以调查
阴道微生物群的种类组成和丰度的年轻成年人与C。沙眼衣原体感染和C.
frac/70/r7 af/s阳性女性盆腔炎(PID)。此外,我们将建立
社区的动态一个妇女小组纵向抽样超过一年或更长时间后,
治疗在这些女性中,我们将使用社区转录组学来识别基因组
由阴道微生物群落表达。这些综合数据将提供阴道的独特视角
衣原体感染期间和之后的微生物群动态(即,代谢途径的详细图像
触发响应感染(直接或间接)],并将进一步我们的衣原体模型
感染和再感染。由于使用人类作为研究对象的局限性,
作为衣原体感染的动物模型。类似地,我们将描述健康女性的阴道微生物群。
和C. cawae感染的雌性豚鼠。理解和描述
阴道微生物群将有助于开发基于rafionale的新方法,
科学上合理的原则来操纵阴道微生物群与治疗平行。
200多个沙眼衣原体或沙眼衣原体的基因组。从这些生物样品中分离的细菌将被
使用454焦磷酸测序进行测序。这些序列将代表一种无与伦比的资源,
与研究界分享。将分析序列数据与阴道的相关性。
微生物群和表型的特点下,该财团的两个项目。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jacques Ravel其他文献
Jacques Ravel的其他文献
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{{ truncateString('Jacques Ravel', 18)}}的其他基金
Host-Microbiota Interactions and STI Outcomes
宿主-微生物群相互作用和性传播感染结果
- 批准号:
10596513 - 财政年份:2021
- 资助金额:
$ 40.51万 - 项目类别:
Host-Microbiota Interactions and STI Outcomes
宿主-微生物群相互作用和性传播感染结果
- 批准号:
10190234 - 财政年份:2021
- 资助金额:
$ 40.51万 - 项目类别:
Host-Microbiota Interactions and STI Outcomes
宿主-微生物群相互作用和性传播感染结果
- 批准号:
10395582 - 财政年份:2021
- 资助金额:
$ 40.51万 - 项目类别:
The Microbial Ecology of Bacterial Vaginosis: A Fine Scale Resolution Metagenomic
细菌性阴道病的微生物生态学:精细分辨率宏基因组学
- 批准号:
8321706 - 财政年份:2011
- 资助金额:
$ 40.51万 - 项目类别:
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