Ecology of the vagina upon Chlamydial infections

与人类阴道相关的微生物群与人类宿主存在互利关系 并被认为对妇女的生殖健康起着重要作用。阴道微生物群落 构成了抵御入侵性非本土生物感染的第一道防线， 性传播疾病，如沙眼衣原体。 尽管它们的重要性，令人惊讶的是，litfie知道阴道微生物的组成和动力学， 在健康和疾病中传播。传统的基于培养的方法提供了一个有价值的，但 人类阴道微生物群的不完整图片。在本研究中，我们将结合 测序技术与16S rRNA基因序列的非培养分析，以调查 阴道微生物群的种类组成和丰度的年轻成年人与C。沙眼衣原体感染和C. frac/70/r7af/s阳性女性盆腔炎（PID）。此外，我们将建立 社区的动态一个妇女小组纵向抽样超过一年或更长时间后， 治疗在这些女性中，我们将使用社区转录组学来识别基因组 由阴道微生物群落表达。这些综合数据将提供阴道的独特视角 衣原体感染期间和之后的微生物群动态（即，代谢途径的详细图像 触发响应感染（直接或间接）]，并将进一步我们的衣原体模型 感染和再感染。由于使用人类作为研究对象的局限性， 作为衣原体感染的动物模型。类似地，我们将描述健康女性的阴道微生物群。 和c. cawae感染的雌性豚鼠。理解和描述 阴道微生物群将有助于开发基于rafionale的新方法， 科学上合理的原则来操纵阴道微生物群与治疗平行。 200多个沙眼衣原体或沙眼衣原体的基因组。从这些生物样品中分离的细菌将被 使用454焦磷酸测序进行测序。这些序列将代表一种无与伦比的资源， 与研究界分享。将分析序列数据与阴道的相关性。 微生物群和表型的特点下，该财团的两个项目。

The microbiota associated with the human vagina exists in a mutualisfic relafionship with the human host and is believed to play an important role in women's reproducfive health. The vaginal microbial communities constitute the first line of defense against infection by invasive non-indigenous organisms that cause disease, such as the sexually transmitted Chlamydia trachomatis. Despite their importance, surprisingly litfie is known about the composition and dynamics of vaginal microbial communifies in health and disease. Traditional culfivafion-based methods have provided a valuable but incomplete picture of the human vaginal microbiota. In this study, we will combined massively-parallel sequencing technology with the culture-independent analysis of the 16S rRNA gene sequence to survey the vaginal microbiota species composifion and abundance in young adults with C. trachomatis infecfion and C. frac/70/r7af/s-positive women with pelvic inflammatory disease (PID). In addifion, we will establish the dynamics of the community a subgroup of women sampled longitudinally over one year or more after treatment. In each of these women, we will use community transcriptomics to identify the suite of genes expressed by the vaginal microbial community. This combined data will afford a unique view of the vaginal microbiota dynamics during and after Chlamydial infecfion (i.e., a detailed picture of the metabolic pathways triggered in response to the infecfions (direcfiy or indirectly)], and will further our model of Chlamydial infecfion and re-ihfecfion. Because of limitations in using humans as research subjects, guinea pigs are used as animal model for Chlamydial infections. Similarlly, we will characterize the vaginal microbiota in healthy and C. cawae-infected female guinea pigs over time. Understand and characterizing the importance of the vaginal microbiota will contribute greafiy to the development of new approaches based on rafionale and scienfifically sound principles to manipulate the vaginal microbiota in parallel to treatments. The genome of more than 200 C.trachomatis or C. caviae isolated from these biological samples will be sequenced using 454 pyrosequencing. These sequences will represent an unparalleled resource that will be shared with the research community. The sequence data will be analyzed in correlafion with the vaginal microbiota and the phenotypes characterized under the two projects of this consortium.