Class II MHC Protein and Tetramer Core

II类MHC蛋白和四聚体核心将支持研究项目1-3和TDC 通过提供II类MHC蛋白和携带A-C类试剂肽的四聚体。的 拟议的核心设施的具体目标是产生HLA-DR 1-肽四聚体， 在定量和表征对A-C类病毒应答的CD 4 + T细胞中， 产生纯化的HLA-DR 1-肽复合物，用于开发微量测定， 检测CD 4 + T细胞，并研究人类II类MHC蛋白的产生， 用作纯化的MHC-肽复合物和四聚体。这些是 商业上无法获得的专用试剂。核心设施的生产将提供 规模经济，标准化生产，试剂检测，并将促进生产 额外的等位基因。HLA-DR-肽蛋白和四聚体的现成可用性应 大大提高了研究项目的枚举、隔离和表征能力 病毒特异性CD 4 + T细胞应答，以及TDC开发和验证新的 鉴定T细胞表位和表征抗原特异性T细胞技术 功能性反应。

The Class II MHC Protein and Tetramer Core will support Research Projects 1-3 and the TDC by providing Class II MHC proteins and tetramers carrying Category A-C agent peptides. The specific aims of the proposed core facility are to generate HLA-DRl-peptide tetramers for use in quantifying and characterizing CD4+ T cells responding to category A-C viruses, to generate purified HLA-DRl-peptide complexes for use in development of microassays to detect CD4+ T cells, and to investigate the production of human class II MHC proteins other than HLA-DR1 for use as purified MHC-peptide complexes and tetramers. These are specialized reagents not available commercially. Production in a core facility will provide economies of scale, standardized production, and reagent testing, and will facilitate production of additional alleles. The ready availability of HLA-DR-peptide proteins and tetramers should greatly enhance the ability of the Research Projects to enumerate, isolate, and characterize virus-specific CD4+ T cell responses, and the ability of the TDC to develop and validate new technology for identification of T cell epitopes and characterization of antigen-specific T cell functional responses.