Class II MHC Protein and Tetramer Core

第二类MHC蛋白和四聚体核心将支持研究项目1-3和贸易发展局 通过提供携带A-C类试剂多肽的II类MHC蛋白和四聚体。这个 拟议核心设施的具体目标是生产供使用的人类白细胞抗原-DRL-多肽四聚体 在量化和表征对A-C类病毒有反应的CD4T细胞时， 制备纯化的人类白细胞抗原-DRL-多肽复合物，用于建立微量分析方法 检测CD4T细胞，并研究人类第二类MHC蛋白的产生 作为纯化的MHC-多肽复合体和四聚体。这些是 专业试剂无法在市场上买到。核心设施的生产将提供 规模经济、标准化生产和试剂检测，并将促进生产 额外的等位基因。人类白细胞抗原-DR多肽蛋白和四聚体的现成供应应该 大大增强研究项目列举、隔离和描述特征的能力 病毒特异性的CD4T细胞反应，以及TDC开发和验证新的 T细胞表位鉴定及抗原特异性T细胞鉴定技术 功能性反应。

The Class II MHC Protein and Tetramer Core will support Research Projects 1-3 and the TDC by providing Class II MHC proteins and tetramers carrying Category A-C agent peptides. The specific aims of the proposed core facility are to generate HLA-DRl-peptide tetramers for use in quantifying and characterizing CD4+ T cells responding to category A-C viruses, to generate purified HLA-DRl-peptide complexes for use in development of microassays to detect CD4+ T cells, and to investigate the production of human class II MHC proteins other than HLA-DR1 for use as purified MHC-peptide complexes and tetramers. These are specialized reagents not available commercially. Production in a core facility will provide economies of scale, standardized production, and reagent testing, and will facilitate production of additional alleles. The ready availability of HLA-DR-peptide proteins and tetramers should greatly enhance the ability of the Research Projects to enumerate, isolate, and characterize virus-specific CD4+ T cell responses, and the ability of the TDC to develop and validate new technology for identification of T cell epitopes and characterization of antigen-specific T cell functional responses.