New Tools for T Cell Identification and Analysis

T 细胞识别和分析的新工具

基本信息

项目摘要

Most clinically relevant blood samples are characterized by a limited number of cells available for analysis combined with a low frequency of the T cell populations of interest. Current methodologies for analysis of these samples often require extensive in vitro manipulation and/or assumptions about the antigen specificity and function of the cell populations of interest. The overall goal of this project is to develop new technology that will improve ex vivo analysis of T cell specificity and function, by taking advantage of advances in bioinformatics, proteomics, protein engineering, and array technology. There are five specific aims. Aim 1 is to improve epitope discovery practice, by developing bioinformatics-based epitope prediction algorithms, by applying recent advances in mass spectrometry to identify naturally processed MHC-bound peptides, and by measuring MHC-peptide kinetic lifetimes rather than equilibrium binding affinities. Aim 2 is to develop novel MHC oligomers to extend tetramer staining technology to characterization of heterologous immune responses and characterization of moderate-affinity and low-avidity T cells. Aim 3 is to develop MHCpeptide arrays and ARC arrays, The arrays will be used for functional characterization of T cells after antigen-specific capture and/or activation. Aim 4 is to optimize ex vivo expansion of T celts using nonspecific expansion and antigen-specific stimulation protocols, and to develop new methods for antigenspecific enrichment, expansion, immortalization of T cell populations. Aim 5 is to develop methodology for high-throughput T cell cloning and analysis, including development of microscale culture methods and application of high-throughput screening methodology to T cell characterization. Once developed and validated, these technologies will be applied to T cell identification and analysis experiments in the associated Research Projects.
大多数临床相关的血液样本的特点是可用于分析的细胞数量有限

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Lawrence J. Stern其他文献

Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water.
枯草杆菌蛋白酶 Carlsberg 在无水乙腈和水中形成的酰基酶中间体的 X 射线晶体结构比较。
Single-Particle Cryo-EM Studies of ERp44-ERAP1 and ERp44-ERAP2 Reveal their ER-Retention Mechanism
  • DOI:
    10.1016/j.bpj.2019.11.2767
  • 发表时间:
    2020-02-07
  • 期刊:
  • 影响因子:
  • 作者:
    Richa Arya;Lawrence J. Stern
  • 通讯作者:
    Lawrence J. Stern
Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen
与超抗原复合的人类 II 类组织相容性分子的三维结构
  • DOI:
    10.1038/368711a0
  • 发表时间:
    1994-04-21
  • 期刊:
  • 影响因子:
    48.500
  • 作者:
    Theodore S. Jardetzky;Jerry H. Brown;Joan C. Gorga;Lawrence J. Stern;Robert G. Urban;Young-in Chi;Cynthia Stauffacher;Jack L. Strominger;Don C. Wiley
  • 通讯作者:
    Don C. Wiley
Chemical inhibition of ER aminopeptidase 1 as a tool for regulating the immunopeptidome of cancer cells
  • DOI:
    10.1016/j.molimm.2022.05.050
  • 发表时间:
    2022-10-01
  • 期刊:
  • 影响因子:
  • 作者:
    Despoina Koumantou;Eilon Barnea;Adrian Martin-Esteban;Zachary Maben;Athanasios Papakyriakou;Paraskevi Kokkala;Harris Pratsinis;Dimitris Georgiadis;Lawrence J. Stern;Arie Admon;Efstratios Stratikos
  • 通讯作者:
    Efstratios Stratikos
Lipid Membrane Association of the T Cell Antigen Receptor ζ Subunit: Affinities and Structure
  • DOI:
    10.1016/j.bpj.2011.11.203
  • 发表时间:
    2012-01-31
  • 期刊:
  • 影响因子:
  • 作者:
    Prabhanshu Shekhar;Kerstin Zimmermann;Mathias Lösche;Lawrence J. Stern;Frank Heinrich
  • 通讯作者:
    Frank Heinrich

Lawrence J. Stern的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Lawrence J. Stern', 18)}}的其他基金

ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
  • 批准号:
    10448371
  • 财政年份:
    2020
  • 资助金额:
    $ 72.6万
  • 项目类别:
ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
  • 批准号:
    10664968
  • 财政年份:
    2020
  • 资助金额:
    $ 72.6万
  • 项目类别:
ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
  • 批准号:
    10045425
  • 财政年份:
    2020
  • 资助金额:
    $ 72.6万
  • 项目类别:
HLA-DO / H2-O: modulation of MHC-II peptide diversity and Treg population control
HLA-DO / H2-O:MHC-II 肽多样性的调节和 Treg 群体控制
  • 批准号:
    10061538
  • 财政年份:
    2017
  • 资助金额:
    $ 72.6万
  • 项目类别:
HLA-DO / H2-O: modulation of MHC-II peptide diversity and Treg population control
HLA-DO / H2-O:MHC-II 肽多样性的调节和 Treg 群体控制
  • 批准号:
    10308470
  • 财政年份:
    2017
  • 资助金额:
    $ 72.6万
  • 项目类别:
CD4 T cell respnse to Human herpesvirus-6
CD4 T 细胞对人类疱疹病毒 6 的反应
  • 批准号:
    9226033
  • 财政年份:
    2014
  • 资助金额:
    $ 72.6万
  • 项目类别:
Small molecule probes of ERAP-1
ERAP-1小分子探针
  • 批准号:
    8446272
  • 财政年份:
    2012
  • 资助金额:
    $ 72.6万
  • 项目类别:
Small molecule probes of ERAP-1
ERAP-1小分子探针
  • 批准号:
    8327981
  • 财政年份:
    2012
  • 资助金额:
    $ 72.6万
  • 项目类别:
NEF_TCRZ_PROJECT
NEF_TCRZ_项目
  • 批准号:
    7957263
  • 财政年份:
    2009
  • 资助金额:
    $ 72.6万
  • 项目类别:
Class II MHC Protein and Tetramer Core
II 类 MHC 蛋白和四聚体核心
  • 批准号:
    7698599
  • 财政年份:
    2008
  • 资助金额:
    $ 72.6万
  • 项目类别:

相似海外基金

Reconstruction algorithms for time-domain diffuse optical tomography imaging of small animals
小动物时域漫射光学断层成像重建算法
  • 批准号:
    RGPIN-2015-05926
  • 财政年份:
    2019
  • 资助金额:
    $ 72.6万
  • 项目类别:
    Discovery Grants Program - Individual
Reconstruction algorithms for time-domain diffuse optical tomography imaging of small animals
小动物时域漫射光学断层成像重建算法
  • 批准号:
    RGPIN-2015-05926
  • 财政年份:
    2018
  • 资助金额:
    $ 72.6万
  • 项目类别:
    Discovery Grants Program - Individual
Reconstruction algorithms for time-domain diffuse optical tomography imaging of small animals
小动物时域漫射光学断层成像重建算法
  • 批准号:
    RGPIN-2015-05926
  • 财政年份:
    2017
  • 资助金额:
    $ 72.6万
  • 项目类别:
    Discovery Grants Program - Individual
Reconstruction algorithms for time-domain diffuse optical tomography imaging of small animals
小动物时域漫射光学断层成像重建算法
  • 批准号:
    RGPIN-2015-05926
  • 财政年份:
    2016
  • 资助金额:
    $ 72.6万
  • 项目类别:
    Discovery Grants Program - Individual
Event detection algorithms in decision support for animals health surveillance
动物健康监测决策支持中的事件检测算法
  • 批准号:
    385453-2009
  • 财政年份:
    2015
  • 资助金额:
    $ 72.6万
  • 项目类别:
    Collaborative Research and Development Grants
Algorithms to generate designs of potency experiments that use far fewer animals
生成使用更少动物的效力实验设计的算法
  • 批准号:
    8810865
  • 财政年份:
    2015
  • 资助金额:
    $ 72.6万
  • 项目类别:
Reconstruction algorithms for time-domain diffuse optical tomography imaging of small animals
小动物时域漫射光学断层成像重建算法
  • 批准号:
    RGPIN-2015-05926
  • 财政年份:
    2015
  • 资助金额:
    $ 72.6万
  • 项目类别:
    Discovery Grants Program - Individual
Event detection algorithms in decision support for animals health surveillance
动物健康监测决策支持中的事件检测算法
  • 批准号:
    385453-2009
  • 财政年份:
    2013
  • 资助金额:
    $ 72.6万
  • 项目类别:
    Collaborative Research and Development Grants
Development of population-level algorithms for modelling genomic variation and its impact on cellular function in animals and plants
开发群体水平算法来建模基因组变异及其对动植物细胞功能的影响
  • 批准号:
    FT110100972
  • 财政年份:
    2012
  • 资助金额:
    $ 72.6万
  • 项目类别:
    ARC Future Fellowships
Advanced computational algorithms for brain imaging studies of freely moving animals
用于自由活动动物脑成像研究的先进计算算法
  • 批准号:
    DP120103813
  • 财政年份:
    2012
  • 资助金额:
    $ 72.6万
  • 项目类别:
    Discovery Projects
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了