New Tools for T Cell Identification and Analysis
T 细胞识别和分析的新工具
基本信息
- 批准号:7701546
- 负责人:
- 金额:$ 72.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-04-01 至 2014-03-31
- 项目状态:已结题
- 来源:
- 关键词:AffinityAlgorithmsAnimalsAntigensAreaAvidityBindingBioinformaticsBiological AssayBiological ModelsBlood specimenCategoriesCell CountCell Culture TechniquesCell physiologyCellular ImmunityCloningDevelopmentElementsEpitopesEquilibriumFlavivirusFrequenciesGoalsHantavirusHerpesviridae InfectionsHumanImmune responseImmune systemImmunityIn VitroKineticsLeukocytesLymphocyte FunctionMHC Class II GenesMHC binding peptideMass Spectrum AnalysisMeasuresMethodologyMethodsPeptide/MHC ComplexPeptidesPopulationPoxviridaeProcessProtein EngineeringProteinsProteomicsProtocols documentationResearch Project GrantsSamplingScreening procedureSpecificityStaining methodStainsStudy modelsSystemT-Cell Immunologic SpecificityT-LymphocyteT-Lymphocyte EpitopesTechnologyTestingVaccinationVirusVirus Diseasesantigen processingbasebiodefenseclinically relevanthigh throughput screeninghuman diseaseimprovedinfluenzavirusinterestnew technologynovelnovel therapeuticspathogenperipheral bloodprogramsresearch studyresponsetechnology developmenttool
项目摘要
Most clinically relevant blood samples are characterized by a limited number of cells available for analysis
combined with a low frequency of the T cell populations of interest. Current methodologies for analysis of
these samples often require extensive in vitro manipulation and/or assumptions about the antigen specificity
and function of the cell populations of interest. The overall goal of this project is to develop new technology
that will improve ex vivo analysis of T cell specificity and function, by taking advantage of advances in
bioinformatics, proteomics, protein engineering, and array technology. There are five specific aims. Aim 1 is
to improve epitope discovery practice, by developing bioinformatics-based epitope prediction algorithms, by
applying recent advances in mass spectrometry to identify naturally processed MHC-bound peptides, and by
measuring MHC-peptide kinetic lifetimes rather than equilibrium binding affinities. Aim 2 is to develop novel
MHC oligomers to extend tetramer staining technology to characterization of heterologous immune
responses and characterization of moderate-affinity and low-avidity T cells. Aim 3 is to develop MHCpeptide
arrays and ARC arrays, The arrays will be used for functional characterization of T cells after
antigen-specific capture and/or activation. Aim 4 is to optimize ex vivo expansion of T celts using nonspecific
expansion and antigen-specific stimulation protocols, and to develop new methods for antigenspecific
enrichment, expansion, immortalization of T cell populations. Aim 5 is to develop methodology for
high-throughput T cell cloning and analysis, including development of microscale culture methods and
application of high-throughput screening methodology to T cell characterization. Once developed and
validated, these technologies will be applied to T cell identification and analysis experiments in the
associated Research Projects.
大多数临床相关的血液样本的特点是可用于分析的细胞数量有限
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Lawrence J. Stern其他文献
Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water.
枯草杆菌蛋白酶 Carlsberg 在无水乙腈和水中形成的酰基酶中间体的 X 射线晶体结构比较。
- DOI:
- 发表时间:
1998 - 期刊:
- 影响因子:11.1
- 作者:
J. Schmitke;Lawrence J. Stern;A. Klibanov - 通讯作者:
A. Klibanov
Single-Particle Cryo-EM Studies of ERp44-ERAP1 and ERp44-ERAP2 Reveal their ER-Retention Mechanism
- DOI:
10.1016/j.bpj.2019.11.2767 - 发表时间:
2020-02-07 - 期刊:
- 影响因子:
- 作者:
Richa Arya;Lawrence J. Stern - 通讯作者:
Lawrence J. Stern
Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen
与超抗原复合的人类 II 类组织相容性分子的三维结构
- DOI:
10.1038/368711a0 - 发表时间:
1994-04-21 - 期刊:
- 影响因子:48.500
- 作者:
Theodore S. Jardetzky;Jerry H. Brown;Joan C. Gorga;Lawrence J. Stern;Robert G. Urban;Young-in Chi;Cynthia Stauffacher;Jack L. Strominger;Don C. Wiley - 通讯作者:
Don C. Wiley
Chemical inhibition of ER aminopeptidase 1 as a tool for regulating the immunopeptidome of cancer cells
- DOI:
10.1016/j.molimm.2022.05.050 - 发表时间:
2022-10-01 - 期刊:
- 影响因子:
- 作者:
Despoina Koumantou;Eilon Barnea;Adrian Martin-Esteban;Zachary Maben;Athanasios Papakyriakou;Paraskevi Kokkala;Harris Pratsinis;Dimitris Georgiadis;Lawrence J. Stern;Arie Admon;Efstratios Stratikos - 通讯作者:
Efstratios Stratikos
Lipid Membrane Association of the T Cell Antigen Receptor ζ Subunit: Affinities and Structure
- DOI:
10.1016/j.bpj.2011.11.203 - 发表时间:
2012-01-31 - 期刊:
- 影响因子:
- 作者:
Prabhanshu Shekhar;Kerstin Zimmermann;Mathias Lösche;Lawrence J. Stern;Frank Heinrich - 通讯作者:
Frank Heinrich
Lawrence J. Stern的其他文献
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{{ truncateString('Lawrence J. Stern', 18)}}的其他基金
ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
- 批准号:
10448371 - 财政年份:2020
- 资助金额:
$ 72.6万 - 项目类别:
ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
- 批准号:
10664968 - 财政年份:2020
- 资助金额:
$ 72.6万 - 项目类别:
ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
- 批准号:
10045425 - 财政年份:2020
- 资助金额:
$ 72.6万 - 项目类别:
HLA-DO / H2-O: modulation of MHC-II peptide diversity and Treg population control
HLA-DO / H2-O:MHC-II 肽多样性的调节和 Treg 群体控制
- 批准号:
10061538 - 财政年份:2017
- 资助金额:
$ 72.6万 - 项目类别:
HLA-DO / H2-O: modulation of MHC-II peptide diversity and Treg population control
HLA-DO / H2-O:MHC-II 肽多样性的调节和 Treg 群体控制
- 批准号:
10308470 - 财政年份:2017
- 资助金额:
$ 72.6万 - 项目类别:
CD4 T cell respnse to Human herpesvirus-6
CD4 T 细胞对人类疱疹病毒 6 的反应
- 批准号:
9226033 - 财政年份:2014
- 资助金额:
$ 72.6万 - 项目类别:
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