DIETARY PROTEIN INTAKE IN MAINTENANCE OF HEMODIALYSIS PATIENTS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 该项目由两项研究组成。 研究1包括9至13名临床稳定的维持性血液透析（MHD）患者，他们将接受氮平衡研究，同时摄入饮食蛋白质，每天提供0.60、0.80、1.00、1.15和1.30 g蛋白质/kg，持续17天。 将进行13 C-亮氨酸转换研究、13 C-尿素动力学研究和血浆氨基酸、透析液氨基酸、肽和蛋白质的测量、肌肉活检（用于测量IGF-I、IGF-II、IGF受体、肌生长抑制素和IGF结合蛋白的蛋白质和基因表达）和身体组成（人体测量和双X射线光子吸收测定法（DXA））。 在研究2中，将70-80名MHD门诊患者随机分配至DPI为1.00或1.30 g/kg/天的组，各持续5个月。将通过测量全身氮和全身钾来评估全身蛋白质。身体组成的其他成分将使用人体测量、DXA、BIA和近红外线相互作用进行评估。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is composed of two studies. Study 1 consists of 9 to 13 maintenance hemodialysis (MHD) patients who are clinically stable and will undergo nitrogen balance studies while they ingest, for 17 days each, diet protein intakes providing 0.60, 0.80, 1.00, 1.15 and 1.30 g protein/kg/day. 13 C-leucine turnover studies 13C-urea kinetic studies and measurements of plasma amino acides, dialysate amino acids, peptides and proteins, muscle biopsies for measurement of protein and gene expression for IGF-I, IGF-II, IGF-receptor, myostatin, and IGF-binding proteins and body composition (anthropometry and dual x-ray photon absorptiometry (DXA)) will be measured. In Study 2, 70-80 MHD outpatients will be randomly assigned to a DPI of 1.00 or 1.30 g/kg/day for five months each. Total body protein will be assessed by measurements of total body nitrogen and total body potassium. Other components of body composition will be assessed using anthropometry, DXA, BIA and near infrared interactance.