ALTERATIONS IN ADIPOCYTOKINE EXPRESSION BEFORE AND AFTER WEIGHT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的主要目的是检查体重减轻前后受试者脂肪细胞衍生的脂肪细胞因子表达的变化。我们假设脂肪细胞产生的代谢介质会随着体重减轻而减少。 受试者将作为门诊患者在波士顿医学中心的营养和体重管理中心招募。符合条件的人群包括18至80岁，体重指数（BMI）大于或等于25 kg/m^2的人群。 减肥治疗方案将由营养和体重管理中心提供，可以包括低脂饮食，有或没有，抗肥胖药物，流质饮食，蛋白质节省修改快速，胃旁路手术。入组者将在基线以及1年观察期内的第3、6和12个月进行4次活检访视。每次活检访视后7天，将要求受试者返回进行缝线拆除访视，以便评估其活检伤口并拆除缝线。在研究期间将进行4对活检和拆线访视（共8次单独访视）。每次活检访视时将进行以下程序：CBC、同型半胱氨酸、基础代谢组、LFT、TSH、C反应蛋白、空腹血糖、胰岛素、脂质（总胆固醇、HDL、LDL、三酰甘油）、HbA 1c、羟基丁酸酯、游离脂肪酸、瘦素、脂联素、TNF-α和派-1的实验室检查值;皮下脂肪活检、DEXA测定的身体组成;以及尿妊娠试验（适用于所有女性）。 将收集以下数据：体重、身高、腰围、BMI和血压。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The principal aim of this study will be to examine alterations in fat cell-derived adipocytokine expression in subjects before and after weight loss. We hypothesize that adipocyte production of metabolic mediators will decrease following weight loss. Subjects will be recruited as outpatients in the Nutrition and Weight Management Center at Boston Medical Center. Eligibility includes those 18 to 80 years old, with a Body Mass Index (BMI) greater than or equal to 25 kg/m^2. Weight loss treatment options will be those offered by the Nutrition and Weight Management Center and can include low fat diets with or without, anti-obesity medications, liquid diets, protein-sparing modified fast, and gastric bypass surgery. Enrollees will have 4 biopsy visits at baseline, and months 3, 6 and 12 during the 1 year observation period. Seven days from each biopsy visit, subjects will be asked to return for a suture removal visit so that their biopsy wounds can be assessed and sutures removed. There will be 4 pairs of biopsy and suture removal visits (8 separate visits total) occurring over the study period. The following procedures will occur at each biopsy visit: laboratory values for CBC, homocysteine, basic metabolic panel, LFT, TSH, C-reactive protein, fasting glucose, insulin, lipids (total cholesterol, HDL, LDL, triacylglycerol), HbA1c, hydroxybutyrate, free fatty acids, leptin, adiponectin, TNF-a and PAI-1; subcutaneous fat biopsy, body composition by DEXA; and urine pregnancy test (for all women). The following will be collected: weight, height, waist circumference, BMI, and blood pressure.