DEVELOPING AN IN VITRO MODEL OF INFECTION OF BOVINE B CELLS WITH BLV

开发 BLV 感染牛 B 细胞的体外模型

• 批准号: 7627623
• 负责人: JEFFRY S ISAACSON
• 金额: $ 4.92万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7627623
• 关键词: Animals; Antibody Formation; B-Lymphocytes; Biological Assay; Bos taurus; Bovine Leukemia Virus; Bovine Lymphosarcoma; Bovine leukemia; Cattle; Cells; Computer Retrieval of Information on Scientific Projects Database; Diffuse Lymphoma; Flow Cytometry; Funding; Grant; Health; Human T-Cell Leukemia Viruses; In Vitro; Infection; Institution; Lymphocytosis; Methods; Numbers; Publications; RNA-Directed DNA Polymerase; Reporting; Research; Research Personnel; Resources; Retroviridae; Source; T-Lymphocyte; United States National Institutes of Health; Viral Antigens; Virus Diseases; in vitro Model; infected B cell; leukemia virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bovine leukemia virus (BLV) is a retrovirus that infects cattle. It is closely related to the human T cell leukemia virus. However, BLV mainly infects B cells, rather than T cells. BLV causes a B cell lymphosarcoma in about 5% of infected animals, but can also cause persistent lymphocytosis and other health problems in a larger percentage of infected cattle. In previous publications, Isaacson et al. reported that cattle experimentally infected with BLV had increased antibody responses to various non-BLV antigens, and a significantly increased number of B cells from these animals were found to express MHC-II molecules. To date, incubation of BLV-negative bovine lymphosarcoma (BL-3) cells with cell-free supernatants from BLV-infected FLK-BLV cells has resulted in the BL-3 cells becoming PCR-positive for BLV. These results are currently being confirmed, and various methods for quantitation of BLV in FLK-BLV supernatants are being investigated. Thus far, attempts to determine BLV titers by a reverse transcriptase assay, as well as by a TCID50 assay using BLV-susceptible CC81 cells, have been unsuccessful, but efforts are continuing. In addition, the BLV-infected BL-3 cells will soon be assayed by flow cytometry to determine whether levels of MHC-II expression are increased following in vitro BLV infection.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 牛白血病病毒（BLV）是感染牛的逆转录病毒。 它与人类T细胞白血病病毒密切相关。 但是，BLV主要感染B细胞，而不是T细胞。 BLV在约5％的感染动物中引起B细胞淋巴瘤，但也可能导致持续的淋巴细胞增多和其他健康问题，而感染的牛则较大。 在以前的出版物中，Isaacson等人。报道说，通过实验感染BLV的牛对各种非BLV抗原的抗体反应增加，并且发现这些动物的B细胞数量显着增加来表达MHC-II分子。 迄今为止，BLV阴性牛淋巴结瘤（BL-3）细胞与来自BLV感染的FLK-BLV细胞的无细胞上清液的细胞孵育，导致BL-3细胞对BLV呈PCR阳性。 目前正在确认这些结果，并且正在研究FLK-BLV上清液中BLV的各种方法。 到目前为止，尝试通过逆转录酶测定以及使用BLV敏感CC81细胞的TCID50分析来确定BLV滴度的尝试一直不成功，但努力仍在继续。 另外，流式细胞仪将很快测定BLV感染的BL-3细胞，以确定体外BLV感染后MHC-II表达水平是否增加。