AR COBRE: MEMBRANE PROTEINS

AR COBRE：膜蛋白

• 批准号: 7609745
• 负责人: denise greathouse
• 金额: $ 34.97万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7609745
• 关键词: Address; Binding; Biochemistry; Biological; Biological Models; Biological Process; Cell physiology; Clinical Medicine; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytochrome P450; Development; Diagnostic; Enzymes; Fluorescence Spectroscopy; Funding; Goals; Grant; Health Benefit; Healthcare; Institution; Intervention; Investigation; Learning; Lipids; Medical; Membrane; Membrane Biology; Membrane Proteins; Methods; Microscopy; Modeling; Proteins; Reaction; Regulation; Research; Research Personnel; Resources; Science; Source; Staging; System; Techniques; Tertiary Protein Structure; United States National Institutes of Health; base; design; frontier; insight; protein function; prototype; solid state

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biophysical investigations are proposed to elucidate key aspects of the functional interactions between proteins and lipids in biological membranes. Membrane proteins are an important frontier within not only biochemistry, but science generally. Membrane protein function has crucial implications for cell physiology and the regulation of biological function. A key goal of this project will be to develop and advance experimental methods in solid-state NMR spectroscopy and fluorescence microscopy that are needed for addressing the mechanisms by which proteins and lipids interact within biological membranes. Due to the complexity of biological membranes, the projects proposed here will, initially, make use of judiciously designed model systems that consist of small, hydrophobic protein domains and that will pave the way for studies of more complex systems. We then will extend the methods to investigate and characterize a prototype system of greater complexity, the membrane-anchored cytochrome P450 enzyme. The model systems provide two means for setting the stage: they allow understanding and insight into fundamental molecular interactions; and they lend themselves toward the development of experimental techniques that then become available for application to other (larger) systems, such as the complexes of P450 with membrane-bound reaction partners, and beyond. Tangible benefits for health care may accrue in two ways: The model and prototype systems themselves may suggest new strategies for membrane-based interventions in diagnostic or clinical medicine. Furthermore, the general membrane principles that are learned may be applied to medical issues in membrane biology.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 生物物理学研究旨在阐明生物膜中蛋白质和脂质之间功能相互作用的关键方面。 膜蛋白不仅是生物化学的一个重要前沿领域，而且是整个科学领域的一个重要前沿领域。 膜蛋白功能对细胞生理学和生物功能的调节具有重要意义。 该项目的一个关键目标是开发和推进固态核磁共振波谱和荧光显微镜的实验方法，这些方法是解决蛋白质和脂质在生物膜内相互作用的机制所必需的。 由于生物膜的复杂性，这里提出的项目最初将利用精心设计的模型系统，该系统由小的疏水蛋白结构域组成，这将为研究更复杂的系统铺平道路。 然后，我们将扩展这些方法来研究和表征更复杂的原型系统，即膜锚定细胞色素 P450 酶。 模型系统提供了两种奠定基础的方法：它们允许理解和洞察基本的分子相互作用；它们有助于实验技术的开发，然后可应用于其他（更大的）系统，例如 P450 与膜结合反应伙伴的复合物等。 医疗保健的切实好处可能通过两种方式产生：模型和原型系统本身可能会为诊断或临床医学中基于膜的干预措施提出新策略。 此外，所学到的一般膜原理可以应用于膜生物学中的医学问题。