AR COBRE: MEMBRANE PROTEINS

AR COBRE：膜蛋白

• 批准号: 7719935
• 负责人: denise greathouse
• 金额: $ 32.6万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7719935
• 关键词: Address; Binding; Biochemistry; Biological; Biological Models; Biological Process; Cell physiology; Clinical Medicine; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytochrome P450; Development; Diagnostic; Enzymes; Fluorescence Spectroscopy; Funding; Goals; Grant; Health Benefit; Healthcare; Institution; Intervention; Investigation; Learning; Lipids; Medical; Membrane; Membrane Biology; Membrane Proteins; Methods; Microscopy; Modeling; Proteins; Reaction; Regulation; Research; Research Personnel; Resources; Science; Source; Staging; System; Techniques; Tertiary Protein Structure; United States National Institutes of Health; base; design; frontier; insight; protein function; prototype; solid state

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biophysical investigations are proposed to elucidate key aspects of the functional interactions between proteins and lipids in biological membranes. Membrane proteins are an important frontier within not only biochemistry, but science generally. Membrane protein function has crucial implications for cell physiology and the regulation of biological function. A key goal of this project will be to develop and advance experimental methods in solid-state NMR spectroscopy and fluorescence microscopy that are needed for addressing the mechanisms by which proteins and lipids interact within biological membranes. Due to the complexity of biological membranes, the projects proposed here will, initially, make use of judiciously designed model systems that consist of small, hydrophobic protein domains and that will pave the way for studies of more complex systems. We then will extend the methods to investigate and characterize a prototype system of greater complexity, the membrane-anchored cytochrome P450 enzyme. The model systems provide two means for setting the stage: they allow understanding and insight into fundamental molecular interactions; and they lend themselves toward the development of experimental techniques that then become available for application to other (larger) systems, such as the complexes of P450 with membrane-bound reaction partners, and beyond. Tangible benefits for health care may accrue in two ways: The model and prototype systems themselves may suggest new strategies for membrane-based interventions in diagnostic or clinical medicine. Furthermore, the general membrane principles that are learned may be applied to medical issues in membrane biology.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 生物物理学的调查，建议阐明生物膜中蛋白质和脂质之间的功能相互作用的关键方面。 膜蛋白质不仅是生物化学领域的一个重要前沿，而且是整个科学领域的一个重要前沿。 膜蛋白功能对细胞生理学和生物学功能的调节具有重要意义。 该项目的一个关键目标是开发和推进固态NMR光谱和荧光显微镜的实验方法，这些方法是解决蛋白质和脂质在生物膜内相互作用的机制所必需的。 由于生物膜的复杂性，这里提出的项目将首先利用明智设计的模型系统，该系统由小的疏水蛋白质结构域组成，这将为更复杂系统的研究铺平道路。 然后，我们将扩展的方法来调查和表征一个原型系统的更大的复杂性，膜锚定细胞色素P450酶。 模型系统提供了两种方法来设置阶段：它们允许理解和洞察基本的分子相互作用;它们有助于实验技术的发展，然后可以应用于其他（更大的）系统，如P450与膜结合反应伙伴的复合物，等等。 对医疗保健的长期益处可能以两种方式积累：模型和原型系统本身可能为诊断或临床医学中的膜基干预提出新的策略。 此外，所学到的一般膜原理可以应用于膜生物学中的医学问题。