The Role of Uncultivated Bacteria in Bacterial Vaginosis
未培养的细菌在细菌性阴道病中的作用
基本信息
- 批准号:7551985
- 负责人:
- 金额:$ 59.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-01-01 至 2010-12-31
- 项目状态:已结题
- 来源:
- 关键词:AffectAftercareAnaerobic BacteriaAntibiotic TherapyAreaBacteriaBacterial VaginosisBase SequenceBehavioralBiological AssayCell CountClinicalCollaborationsCollectionCommunitiesComplementComplexConfocal MicroscopyConsensus SequenceDNA SequenceDataDetectionDiagnosisDiagnosticDiseaseEcologyEcosystemEnvironmentEpidemiologyEpithelialEscherichia coliFemale of child bearing ageFluorescence MicroscopyFluorescent in Situ HybridizationFred Hutchinson Cancer Research CenterGardnerella vaginalisGastrointestinal tract structureGenesGoalsGrantHIV InfectionsHeadHealthHumanInfectionKnowledgeLaboratoriesLactobacillusLibrariesLinkMeasuresMegasphaeraMethodologyMethodsMicrobeMicrobiologyMicroscopicMinorityMobiluncus curtisiiMolecularMolecular Diagnostic TechniquesMolecular EpidemiologyMucous MembraneNatural HistoryNucleic acid sequencingOral cavityOral mucous membrane structureOutcomePathogenesisPatientsPelvic Inflammatory DiseasePlayPopulationPremature LaborPrevalencePreventionReagentRecombinant DNARectumRelapseResearch InfrastructureResearch PersonnelResistanceResource SharingRibosomal DNARisk FactorsRoleSamplingSequence AnalysisSex BehaviorSexually Transmitted DiseasesSpecimenSurfaceSwabTissuesUnsafe SexVaginaVaginal DischargeWomanWomen&aposs Healthbacteria characteristiccohortexperienceimprovedmeetingsmicrobialmicrobial communitynovelpathogenpreventprogramsrectalreproductive successtoolvaginal fluid
项目摘要
Bacterial vaginosis (BV) is a condition that affects millions of women, produces malodorous vaginal discharge, and is
linked to several serious health conditions including preterm labor and HIV infection. The cause of BV is unclear,
though changes in vaginal flora are noted and patients may respond to antibiotic treatment. No single cultivated
bacterium is specifically associated with BV. Bacteria in complex microbial communities can be identified without
cultivation by characterizing ribosomal DNA sequences. This approach offers the advantage of detecting fastidious or
cultivation-resistant microbes. The short-term objective of this project is to use cultivation-independent molecular
methods to characterize the vaginal flora of women with BV and healthy controls. The long-term objectives are to
understand the bacterial ecology of the vaginal niche and determine the role uncultivated bacteria play in BV.
Hypothesis: novel communities of bacteria, including many uncultivated species, are found in subjects with BV. To
meet these objectives, the following aims will be pursued: Aim 1:Identify the community of bacteria associated with
BV. Broad range PCR will be used to amplify bacterial 16SrRNA genes present in vaginal fluid. Sequence analysis of
the amplified 16SrDNA will be used to identify the bacterial species found in subjects with BV and healthycontrols.
Aim 2: Usebacterium specific PCR and fluorescence in situ hybridization (FISH) assays to detect and quantify each
vaginal bacterium. Bacterium-specificPCR and FISH assays are more sensitive than broad range PCR for detecting
infrequent bacterial species in complex communities. These methods will be applied to vaginal fluid samples from
additional subjects with BV and healthy controls. FISH paired with fluorescence microscopy will be used to count
bacteria in vaginal fluid smears, thus producing an independent measure of bacterial representation to complement PCR
methods. Aim3:Determine if there is an extra-vaginal niche for BVassociated bacteria. The gastrointestinal tract
hosts numerous anaerobic bacteria and may be a reservoir for the bacteria linked to BV. Swabs ofextra-vaginal
mucosal surfaces will be obtained from women with and without BV. Bacteria on these swabs will be detectedusing
bacterium-specific PCRs. We hypothesize that BV-associated bacteria will be common colonizers of thehuman
gastrointestinal tract. Knowledge gained from this project will help identify the bacterial communities found in subjects
with BV, will generate opportunities to advance the diagnosis of BV through new PCR and FISH assays, and may help
in the prevention of BV by identifying extra-vaginal reservoirs of infection.
细菌性阴道病(BV)是一种影响数百万妇女的疾病,产生恶臭的阴道分泌物,
与几种严重的健康状况有关,包括早产和艾滋病毒感染。BV的原因尚不清楚,
尽管注意到阴道植物群的变化,并且患者可能对抗生素治疗有反应。没有单一的栽培
细菌与BV特异性相关。复杂微生物群落中的细菌可以在没有
通过表征核糖体DNA序列进行培养。这种方法提供了检测挑剔或
耐培养微生物。本项目的短期目标是利用非培养依赖性分子生物学技术,
方法描述BV患者和健康对照组的阴道植物群。长期目标是
了解阴道生态位的细菌生态,并确定未培养的细菌在BV中的作用。
假设:在BV受试者中发现了新的细菌群落,包括许多未培养的菌种。到
为了实现这些目标,将追求以下目标:目标1:确定与
BV.将使用宽范围PCR扩增阴道液中存在的细菌16SrRNA基因。序列分析
扩增的16SrDNA将用于鉴定BV受试者和健康对照中发现的细菌种类。
目的2:细菌特异性PCR和荧光原位杂交(FISH)检测和定量
阴道细菌细菌特异性PCR和FISH检测比广谱PCR更敏感,
复杂群落中罕见的细菌物种。这些方法将应用于阴道液样本,
其他BV受试者和健康对照。将使用FISH与荧光显微镜配对进行计数
阴道液涂片中的细菌,从而产生细菌代表性的独立测量,以补充PCR
方法.目的3:确定BV相关细菌是否存在阴道外小生境。胃肠道
宿主有许多厌氧细菌,并且可能是与BV相关的细菌的储存库。阴道外拭子
粘膜表面将从患有和不患有BV的女性获得。这些拭子上的细菌将使用
细菌特异性PCR。我们假设BV相关细菌将是人类的常见定植者,
胃肠道从这个项目中获得的知识将有助于确定在受试者中发现的细菌群落
与BV,将产生机会,以推进BV的诊断,通过新的PCR和FISH检测,并可能有助于
通过识别阴道外感染库来预防BV。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DAVID Neal FREDRICKS其他文献
DAVID Neal FREDRICKS的其他文献
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{{ truncateString('DAVID Neal FREDRICKS', 18)}}的其他基金
Fecal Microbiota Transplantation and Fiber for the Treatment of Graft-versus-host Disease After Hematopoietic Cell Transplantation
粪便微生物群移植和纤维治疗造血细胞移植后移植物抗宿主病
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$ 59.35万 - 项目类别:
ANAEROBE 2022: the 16th Biennial Congress of the Anaerobe Society of the Americas (ASA)
厌氧菌 2022:第 16 届美洲厌氧菌协会 (ASA) 双年度大会
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10464618 - 财政年份:2022
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Prospective Epidemiologic Study of Novel Etiologic Agents of Pelvic Inflammatory Disease
盆腔炎新病因的前瞻性流行病学研究
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10668432 - 财政年份:2020
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$ 59.35万 - 项目类别:
Prospective Epidemiologic Study of Novel Etiologic Agents of Pelvic Inflammatory Disease
盆腔炎新病因的前瞻性流行病学研究
- 批准号:
10220681 - 财政年份:2020
- 资助金额:
$ 59.35万 - 项目类别:
Prospective Epidemiologic Study of Novel Etiologic Agents of Pelvic Inflammatory Disease
盆腔炎新病因的前瞻性流行病学研究
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10471222 - 财政年份:2020
- 资助金额:
$ 59.35万 - 项目类别:
The Gut Microbiota and Graft versus Host Disease (GVHD)
肠道微生物群和移植物抗宿主病 (GVHD)
- 批准号:
10593458 - 财政年份:2017
- 资助金额:
$ 59.35万 - 项目类别:
The Gut Microbiota and Graft versus Host Disease (GVHD)
肠道微生物群和移植物抗宿主病 (GVHD)
- 批准号:
10287495 - 财政年份:2017
- 资助金额:
$ 59.35万 - 项目类别:
The Gut Microbiota and Graft versus Host Disease (GVHD)
肠道微生物群和移植物抗宿主病 (GVHD)
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10053303 - 财政年份:2017
- 资助金额:
$ 59.35万 - 项目类别:
Male urethritis: Novel etiologies and natural history
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- 资助金额:
$ 59.35万 - 项目类别:
Male urethritis: Novel etiologies and natural history
男性尿道炎:新的病因和自然史
- 批准号:
9001246 - 财政年份:2014
- 资助金额:
$ 59.35万 - 项目类别:
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