Serum Antibodies to Recombinant Pneumocystis Antigens

重组肺孢子虫抗原的血清抗体

• 批准号: 7585265
• 负责人: PETER D WALZER
• 金额: $ 46.77万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7585265
• 关键词: AIDS clinical trial group; Acquired Immunodeficiency Syndrome; Acute; Adult; Alveolar Macrophages; Animal Model; Antibodies; Antibody Formation; Antigens; Area; Biological Process; Blood donor; CD4 Lymphocyte Count; Cellular Immunity; Cessation of life; Child; Cohort Studies; Data; Diagnosis; Enzyme-Linked Immunosorbent Assay; Epidemiology; Epitopes; Geographic Locations; Goals; HIV; Highly Active Antiretroviral Therapy; Host Defense; Human; Humoral Immunities; Immune response; Immunocompromised Host; Immunology; Infection; Integration Host Factors; International; Lead; Length; Location; Measures; Membrane Glycoproteins; Organism; Parents; Patients; Phagocytosis; Play; Pneumocystis; Pneumonia; Population; Predisposition; Protease Inhibitor; Reagent; Recombinants; Recovery; Recurrence; Role; Seroepidemiologic Studies; Serological; Serum; T-Lymphocyte Epitopes; Time; Variant; Viral Load result; defense response; extracellular; macrophage; response; tool

DESCRIPTION (provided by applicant): Pneumocystis (Pc) is a major cause of lethal pneumonia (P) in HIV patients, but seroepidemiological studies have been severely limited by the lack of suitable reagents. I have developed recombinant fragments (A, B,C) of the human PC major surface glycoprotein (Msg) and found that one of them, MsgC, shows considerable promise as an antigen for these studies in an enzyme linked immunosorbent assay (ELISA). I hypothesize that MsgC is a universal serologic marker for PC infection, and that the highest level of antibodies will be found in HIV patients who recovered from PcP (PcP+). The goals of this proposal are to analyze serum antibody responses, as well as the host factors that influence these responses, to the Msg fragments in adult HIV PcP+ patients, HIV patients who never had PcP (PcP-), and healthy blood donors in 8 different international locations. Data obtained from these studies will lead to the first standard antigen for PC serologic studies, and enhance our understanding of the epidemiology and immunology of PC infection.

描述（由申请人提供）：肺孢子虫（Pc）是HIV患者中致命性肺炎（P）的主要原因，但由于缺乏合适的试剂，血清流行病学研究受到严重限制。我开发了人类 PC 主要表面糖蛋白 (Msg) 的重组片段 (A、B、C)，并发现其中之一 MsgC 在酶联免疫吸附测定 (ELISA) 中作为这些研究的抗原显示出相当大的前景。我假设 MsgC 是 PC 感染的通用血清学标志物，并且在从 PcP (PcP+) 恢复的 HIV 患者中会发现最高水平的抗体。该提案的目标是分析 8 个不同国际地点的成年 HIV PcP+ 患者、从未患过 PcP (PcP-) 的 HIV 患者和健康献血者的血清抗体反应以及影响这些反应的宿主因素对 Msg 片段的反应。从这些研究中获得的数据将产生用于 PC 血清学研究的第一个标准抗原，并增强我们对 PC 感染的流行病学和免疫学的理解。

项目成果

Geographical variation in serological responses to recombinant Pneumocystis jirovecii major surface glycoprotein antigens.

• DOI: 10.1111/j.1469-0691.2009.02716.x
• 发表时间: 2009-10
• 期刊: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
• 作者: Daly K;Koch J;Respaldiza N;de la Horra C;Montes-Cano MA;Medrano FJ;Varela JM;Calderon EJ;Walzer PD
• 通讯作者: Walzer PD