Geographical variation in serological responses to recombinant Pneumocystis jirovecii major surface glycoprotein antigens.
Geographical variation in serological responses to recombinant Pneumocystis jirovecii major surface glycoprotein antigens.
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DOI：
10.1111/j.1469-0691.2009.02716.x
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Walzer PD
中科院分区：
文献类型：
--
作者：
Daly K;Koch J;Respaldiza N;de la Horra C;Montes-Cano MA;Medrano FJ;Varela JM;Calderon EJ;Walzer PD
使用重组片段的主要表面糖蛋白（Msg）的耶氏肺孢子虫已被证明是有用的研究献血员和人类免疫缺陷病毒（HIV）阳性（HIV+）患者的血清免疫反应。在这里，我们已经使用ELISA来测量抗体滴度Msg片段（MsgA，MsgB，MsgC 1，MsgC 3，MsgC 8和MsgC 9）在美国和西班牙分离的血清中，以确定地理位置是否影响这些抗原的血清学反应。与辛辛那提（美国）献血者相比，来自塞维利亚的献血者对MsgC 8的抗体滴度显著更高，对MsgC 3和MsgC 9的应答显著更低。与西班牙HIV+患者相比，西班牙献血者对MsgC 1、MsgC 8和MsgC 9的反应也有所升高。患有肺孢子虫肺炎（PcP+）的HIV+患者对MsgC 8的反应高于HIV+ PcP-患者。这些数据表明，地理位置在Msg片段的响应性中起作用。此外，这些片段在区分患者群体方面具有实用性。
The use of recombinant fragments of the major surface glycoprotein (Msg) of Pneumocystis jirovecii has proven useful for studying serological immune responses of blood donors and human immunodeficiency virus (HIV)-positive (HIV+) patients. Here, we have used ELISA to measure antibody titres to Msg fragments (MsgA, MsgB, MsgC1, MsgC3, MsgC8 and MsgC9) in sera isolated in the USA and Spain, to determine whether geographical location affects serological responses to these antigens. Blood donors from Seville exhibited a significantly greater antibody titre to MsgC8, and significantly lower responses to MsgC3 and MsgC9, than did Cincinnati (USA) donors. Spanish blood donors also exhibited elevated responses to MsgC1, MsgC8 and MsgC9 as compared with Spanish HIV+ patients . HIV+ patients who had Pneumocystis pneumonia (PcP+) exhibited a higher response to MsgC8 than did HIV+ PcP- patients. These data show that geographical location plays a role in responsiveness to Msg fragments. Additionally, these fragments have utility in differentiating between patient populations.
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影响因子：
--
作者：
Laursen, AL;Andersen, PL
通讯作者：
Andersen, PL
影响因子：
6.4
作者：
Gigliotti, F;Garvy, BA;Harmsen, AG
通讯作者：
Harmsen, AG
影响因子：
6.4
作者：
Gigliotti, F;Harmsen, AG
通讯作者：
Harmsen, AG
影响因子：
9.4
作者：
MADDISON, SE;HAYES, GV;NORMAN, LG
通讯作者：
NORMAN, LG
影响因子：
3.1
作者：
Gigliotti, F;Haidaris, CG;Harmsen, AG
通讯作者：
Harmsen, AG