Global Serological Study of Pneumocystis Infection

肺孢子虫感染的全球血清学研究

• 批准号: 6934161
• 负责人: PETER D WALZER
• 金额: $ 6.74万
• 依托单位: LONDON SCH/HYGIENE & TROPICAL MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6934161
• 关键词: Global; Serological; Study; Pneumocystis; Infection

DESCRIPTION (provided by applicant): Pneumocystis (Pc) is a major cause of lethal pneumonia (P) in HIV patients, but seroepidemiological studies have been severely limited by the lack of suitable reagents. I have developed recombinant fragments (A, B,C) of the human PC major surface glycoprotein (Msg) and found that one of them, MsgC, shows considerable promise as an antigen for these studies in an enzyme linked immunosorbent assay (ELISA). I hypothesize that MsgC is a universal serologic marker for PC infection, and that the highest level of antibodies will be found in HIV patients who recovered from PcP (PcP+). The goals of this proposal are to analyze serum antibody responses, as well as the host factors that influence these responses, to the Msg fragments in adult HIV PcP+ patients, HIV patients who never had PcP (PcP-), and healthy blood donors in 8 different international locations. Data obtained from these studies will lead to the first standard antigen for PC serologic studies, and enhance our understanding of the epidemiology and immunology of PC infection.

描述（由申请人提供）：肺囊虫（Pc）是HIV患者致死性肺炎(P)的主要原因，但由于缺乏合适的试剂，血清流行病学研究受到严重限制。我已经开发了人PC主表面糖蛋白（Msg）的重组片段（A， B，C），并发现其中一个MsgC在酶联免疫吸附试验（ELISA）中显示出相当大的希望作为这些研究的抗原。我假设MsgC是PC感染的通用血清学标记物，并且在PcP （PcP+）恢复的HIV患者中发现最高水平的抗体。本研究的目的是分析8个不同国家的成年HIV PcP+患者、从未患过PcP的HIV患者（PcP-）和健康献血者对味精片段的血清抗体反应，以及影响这些反应的宿主因素。从这些研究中获得的数据将导致PC血清学研究的第一个标准抗原，并增强我们对PC感染的流行病学和免疫学的理解。