MACROMOLECULAR CRYSTALLOGRAPHY REMOTE ACCESS WORKSHOP IN BUFFALO, NEW YORK

纽约州布法罗大分子晶体学远程访问研讨会

• 批准号: 7598089
• 负责人: AINA COHEN
• 金额: $ 1.19万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598089
• 关键词: Buffaloes; Computer Retrieval of Information on Scientific Projects Database; Computer software; Computers; Crystallography; Data Collection; Educational workshop; Funding; Grant; Hand; Ice; Institution; Learning; Liquid substance; Medical; Monitor; New York; Nitrogen; Participant; Protocols documentation; Research; Research Institute; Research Personnel; Resources; Safety; Safety Glasses; Sampling; Shipping; Ships; Source; Students; System; Time; United States National Institutes of Health; computerized data processing; detector; laptop

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. On August 4th 2006, a Remote Access Data Collection workshop was conducted at the Hauptman-Woodward Medical Research Institute (HWI) in Buffalo, New York. Aina Cohen and Clyde Smith from SSRL and Edward Snell (HWI) organized the workshop that was attended by 34 US and Canadian investigators and graduate students. The workshop started off with a presentation by Aina Cohen that introduced the macromolecular crystallography facilities available at SSRL including the Stanford automated mounting system and the associated cassette system used to transport samples. Attendees also learned how to apply for beam time. Following this, a practical demonstration of the control with Blu-Ice, monitoring and data processing software was presented. The attendees learned how to properly prepare sample pins for automated data collection and how to load and ship sample cassettes. Then, after going over proper safety protocols for liquid nitrogen use, the group donned safety shields, safety glasses and gloves and mounted samples under liquid nitrogen in SAM cassettes. Hands-on practcal sessions also covered seting up the remote access software on the participants laptop computers. Finally, participants were able to control beam lines at SSRL remotely; mounting crystals, moving detectors, changing wavelengths etc. They also were able to collect real x-ray data and process it using SSRL computers.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 2006 年 8 月 4 日，远程访问数据收集研讨会在纽约州布法罗的 Hauptman-Woodward 医学研究所 (HWI) 举行。 SSRL 的 Aina Cohen 和 Clyde Smith 以及 Edward Snell (HWI) 组织了这次研讨会，有 34 名美国和加拿大的研究人员和研究生参加。研讨会以 Aina Cohen 的演讲开始，介绍了 SSRL 提供的大分子晶体学设施，包括斯坦福自动安装系统和用于运输样品的相关盒式系统。与会者还学习了如何申请光束时间。 随后，对 Blu-Ice 控制、监控和数据处理软件进行了实际演示。 与会者学习了如何正确准备用于自动数据收集的样品针以及如何加载和运输样品盒。 然后，在仔细研究了液氮使用的正确安全协议后，该小组戴上了安全防护罩、安全眼镜和手套，并将样品安装在 SAM 盒中的液氮下。 实践课程还包括在参与者的笔记本电脑上设置远程访问软件。最后，参与者能够远程控制 SSRL 的光束线；安装晶体、移动探测器、改变波长等。他们还能够收集真实的 X 射线数据并使用 SSRL 计算机进行处理。