MACROMOLECULAR CRYSTALLIZATION, SAMPLE PREPARATION AND SYNCHROTRON DATA COLLECTI

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该研讨会面向目前利用SSRL大分子结晶学束线获取感兴趣目标的结构信息的生物学受众，或者计划开始使用SSRL束线的一些结晶学工作。讲习班的重点是针对那些项目将在近期和中期受益于结构信息的人。讲座涵盖了结晶学的基础知识，强调了高质量晶体的重要性，概述了Hauptman-Woodward医学研究所(HWI)为普通社区提供的高通量筛选服务及其在可溶性和最近的膜蛋白中的应用，优化实验室中的初始结晶条件，以及为同步加速器准备晶体。随后举行了动手实践会议，介绍了如何使用SSRL的远程数据收集设施(包括Blu-Ice和Web-Ice)，并利用现有的工具对晶体进行冷冻、储存和运输到SSRL，以便尽可能收集最佳的X射线数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This workshop was intended for a biological audience who either currently makes use of SSRL macromolecular crystallography beam lines to obtain structural information on targets of interest, or had plans to initiate some crystallographic work using SSRL beam lines. The workshop was focused at a practical level for those with projects that would benefit from structural information in the near to intermediate term. Lectures covered the basics of crystallography, emphasizing the importance of good quality crystals, an overview of the high-throughput screening service available to the general community at the Hauptman-Woodward Medical Research Institute (HWI) and its application to both soluble and very recently membrane proteins, optimization of initial crystallization conditions in the laboratory, along with getting crystals ready for the synchrotron. Hands-on practical sessions followed, describing the use of remote data collection facilities at SSRL (including Blu-Ice and Web-Ice) and making use of the tools available to cryo-cool, store and transport the crystals to SSRL in order to collect the best x-ray data possible.