CRYSTALLOGRAPHY MADE EASY WORKSHOP AT THE SSRL/LCLS USERS? MEETING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该研讨会的目的是向X射线晶体学领域的非专家介绍该技术的基础知识，并告知他们结晶，同步加速器数据收集和数据分析领域的最新技术发展，使大分子结构测定变得不那么繁琐，对初学者来说更简单。 讲习班的讲师包括来自HWI、斯坦福大学、加州大学富勒顿分校、匹兹堡大学和SSRL的科学家。研讨会的课程从晶体学基础的讲座开始。 然后，与会者学习了如何利用实验自动化和设施的最新进展来简化大部分过程。 他们学会了如何正确制备蛋白质并将其发送到HWI进行自动结晶条件筛选和结晶条件优化。 他们获得了将样本装载到卡匣和uni-pucks中以将样本运送到同步加速器的实践经验。 通过与SSRL控制系统的连接，他们学会了如何使用SSRL的自动化设施快速测试晶体的衍射质量，并获得了如何申请自己的远程访问光束时间的信息。 SSRL研讨会的参与者共计约20人。 与会者对他们所学到的东西非常热情，许多人表示有兴趣在SSRL获得束流时间

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this workshop was to introduce non-experts in the field of x-ray crystallography to the basics of the technique and to inform them about recent technology developments in the areas of crystallization, synchrotron data collection and data analysis that make macromolecular structure determination less tedious and more straightforward for beginners. Workshop lecturers included scientists from HWI, Stanford University, UC Fullerton, the University of Pittsburgh, and SSRL. The workshop program began with lectures on the fundamentals of crystallography. Participants then learned how to take advantage of recent advances in experimental automation and facilities to simplify much of the process. They learned how to properly prepare and send protein to HWI for automated crystallization condition screening and crystallization condition optimization. They received hands-on experience on loading samples into cassettes and uni-pucks for shipping samples to the synchrotron. Connecting to the SSRL control system, they learned how to rapidly test crystals for diffraction quality using the automated facilities at SSRL and were provided with information on how to apply for their own remote access beam time. The SSRL workshop participants totaled ~20. Participants were very enthusiastic about what they had learned and many expressed interest in obtaining beam time at SSRL