TUNABLE X-RAY CANCER PHOTOTHERAPY

可调谐 X 射线癌症光疗

• 批准号: 7598125
• 负责人: NEVILLE C LUHMANN
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598125
• 关键词: Biological Assay; Bioluminescence; Cancer Detection; Computer Retrieval of Information on Scientific Projects Database; Count; Cultured Cells; Development; Dose; Elements; Funding; Gadolinium; Gold; Grant; Heavy Metals; Hour; In Vitro; Indium; Institution; Iodine; Label; Laboratories; Laboratory Study; Light; Malignant Neoplasms; Object Attachment; Photons; Phototherapy; Platinum; Radiation; Radiation therapy; Radiation-Sensitizing Agents; Research; Research Personnel; Resources; Sample Size; Source; Testing; Time; United States National Institutes of Health; Work; beamline; established cell line; killings; neoplastic cell; programs; research study; response; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We wish to test the hypothesis that monochromatic x-ray radiation at and above the K-edge of various heavy metals bonded to substrates within neoplastic cells is more effective that conventional radiation therapy for killing neoplastic cells in vitro. This program is part of the Compton Light Source (CLS)project at the SLAC Klystron Department to develop a tunable, compact source of quasi-monoenergetic x-rays for use in cancer detection and treatment. While the CLS is under development, we propose to use a beamline at the Stanford Synchrotron Radiation Laboratory (SSRL) for studying dose enhancement in x-ray phototherapy. The proposed work will use an SSRL beamline to irradiate established cell lines labeled with high-Z atoms. Dose enhancement will be assessed through dose-response curves generated both by traditional colony counting and by bioluminescence assay. Candidates for radiosensitizing agents include Iodine, Gadolinium, Indium, Platinum, and Gold. Saples will be irradiated at energies 0-30 keV above the K-edges of these elements. Although SSRL beamlines produce relatively low fluxes at these energies, cell culture geometry can be adjusted to the small beam size, and samples may be irradiated for long times (hours) to reach higher integrated photon intensities. Broader bandwidths (up to 1 keV) are permitted in these experiments as a trade-off for reaching higher fluxes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们希望检验以下假设：在肿瘤细胞内与基质结合的各种重金属的 K 边缘及其上方的单色 X 射线辐射比传统放射疗法在体外杀死肿瘤细胞更有效。 该项目是 SLAC 速调管部门康普顿光源 (CLS) 项目的一部分，旨在开发可调谐、紧凑的准单能 X 射线源，用于癌症检测和治疗。 虽然 CLS 正在开发中，但我们建议使用斯坦福同步辐射实验室 (SSRL) 的光束线来研究 X 射线光疗中的剂量增强。 拟议的工作将使用 SSRL 光束线照射用高 Z 原子标记的已建立的细胞系。 剂量增强将通过传统菌落计数和生物发光测定生成的剂量反应曲线进行评估。 放射增敏剂的候选材料包括碘、钆、铟、铂和金。 树苗将以高于这些元素的 K 边缘 0-30 keV 的能量进行辐照。 尽管 SSRL 光束线在这些能量下产生相对较低的通量，但细胞培养几何形状可以调整为小光束尺寸，并且可以长时间（数小时）照射样品以达到更高的集成光子强度。 这些实验允许更宽的带宽（高达 1 keV），作为达到更高通量的权衡。