SOLUTION STRUCTURE OF APOLIPOPROTEIN PHOSPHOLIPID COMPLEXES WITH DIFFERENT PHOSP

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 载脂蛋白A-1（apoA-I）和载脂蛋白E（apoE）通过促进胆固醇的加工和转运，在延缓动脉粥样硬化的发展中起重要作用。已显示ApoA-I和apoE磷脂复合物模拟含有任一载脂蛋白的小HDL脂蛋白的生物活性。我们以前对apoE和apoA-I磷脂复合物的研究表明，apoA-I二肉豆蔻酰磷脂酰胆碱（DMPC）和apoE二棕榈酰磷脂酰胆碱（DPPC）复合物的形态在整体形状、蛋白质分布和磷脂堆积方面是不同的。我们希望使用小角散射来绘制apoA-I-DPPC、apoA-I-POPC和apoE-POPC复合物中的蛋白质分布，以确定这些差异是否是由于蛋白质或磷脂的性质造成的。我们还想确定向颗粒中加入游离胆固醇对颗粒形态的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apolipoprotein A-1 (apoA-I) and apolipoprotein E (apoE) play an important role in retarding the development of atherosclerosis by facilitating the processing and transportation of cholesterol. ApoA-I and apoE phospholipid complexes have been shown to mimic the biological activities of small HDL lipoproteins containing either apolipoprotein. Our previous work with apoE and apoA-I phospholipid complexes has suggested that the morphology of a complex of apoA-I dimyristoylphosphatidylcholine (DMPC)and apoE dipalmitoylphosphatidylcholine (DPPC) are different in overall shape, protein distribution and phospholipid packing. We would like to use small angle scattering to map the protein distribution in complexes of apoA-I-DPPC, apoA-I-POPC and apoE-POPC to determine if these differences are due to properties of the protein or the phospholipid. We would also like to determine the effect of the addition of free cholesterol to the particles on particle morphology.