STRUCTURES OF THE E COLI 70S RIBOSOME IN FUNCTIONAL COMPLEXES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 蛋白质的生物合成或翻译发生在核糖体上，核糖体是一种RNA-蛋白质复合体，在所有形式的生命中都是保守的。翻译需要核糖体的大亚基和小亚基协同工作。因此，我们实验室正在通过测定核糖体功能复合体的X射线晶体结构来研究细菌核糖体的作用机制。这些复合体包含mRNA、tRNA、抗生素和翻译因子，它们被困在蛋白质合成的不同步骤中。目前，我们正在研究与核糖体循环因子(RRF)和抑制转位和核糖体循环的抗生素形成的复合体中的核糖体结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Protein biosynthesis, or translation occurs on the ribosome, an RNA-protein complex conserved in all forms of life. Translation requires both the small and large subunits of the ribosome to work in concert. Therefore, our lab is studying the mechanism of the bacterial ribosome by determining x-ray crystal structures of ribosomal functional complexes. These complexes contain mRNA, tRNAs, antibiotics and translation factors trapped in different steps of protein synthesis. Presently, we are working on the structures of the ribosome in complexes with ribosome recycling factor (RRF) and with antibiotics that inhibit translocation and ribosome recycling.