ULTRAFAST MEASUREMENT PROCESSES

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的目的是进一步开发和维护最先进的快速和超快光学和红外光谱方法，这些方法对于研究尽可能广泛的时间尺度范围内的各种生物过程是必不可少的。 该工作的一部分旨在开发用于激发态寿命和各向异性测量的新技术，使用自发发射作为激发态的检测。在与 Burgess 教授的合作实验中，该方法将用于确定 DNA 探针的激发态特性。还建议将 NOPA 装置合并到超快荧光上转换装置中，这对于 DNA 标记盒的动态分析特别重要。例如，在这些研究中，重要的是采用专门调谐到 490 nm 附近能量供体发色团吸收最大值的激发波长，而仅使用 Ti:S 输出的二次谐波无法获得该波长。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this project is to further develop and maintain state of the art fast and ultrafast methods of optical and infrared spectroscopy that are indispensable for the study of a wide range of biological processes stretched over the widest possible range of time scales. Part of the work is aiming at developing new techniques for excited state lifetime and anisotropy measurements that use spontaneous emission as the detection of the excited state. This method will be applied to determine the excited state properties of DNA probes in collaborative experiments with Prof. Burgess. It is also proposed to incorporate a NOPA device into the ultrafast fluorescence upconversion apparatus which would be particularly important in the dynamical analysis of the DNA-labeling cassettes. For example, in those studies, it would important to employ excitation wavelengths specifically tuned to the absorption maxima of the energy donor chromophores near 490 nm, a wavelength not available by simply using the second harmonic of a Ti:S output.