ULTRAFAST MEASUREMENT PROCESSES

超快测量过程

• 批准号: 7598433
• 负责人: ROBIN Main HOCHSTRASSER
• 金额: $ 0.79万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598433
• 关键词: Anisotropy; Arts; Biological Process; Computer Retrieval of Information on Scientific Projects Database; DNA Probes; DNA analysis; Detection; Devices; Fluorescence; Funding; Grant; Institution; Label; Measurement; Methods; Optical Methods; Output; Process; Property; Range; Research; Research Personnel; Resources; Source; Stretching; Techniques; Time; United States National Institutes of Health; Work; absorption; chromophore; infrared spectroscopy; research study; second harmonic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this project is to further develop and maintain state of the art fast and ultrafast methods of optical and infrared spectroscopy that are indispensable for the study of a wide range of biological processes stretched over the widest possible range of time scales. Part of the work is aiming at developing new techniques for excited state lifetime and anisotropy measurements that use spontaneous emission as the detection of the excited state. This method will be applied to determine the excited state properties of DNA probes in collaborative experiments with Prof. Burgess. It is also proposed to incorporate a NOPA device into the ultrafast fluorescence upconversion apparatus which would be particularly important in the dynamical analysis of the DNA-labeling cassettes. For example, in those studies, it would important to employ excitation wavelengths specifically tuned to the absorption maxima of the energy donor chromophores near 490 nm, a wavelength not available by simply using the second harmonic of a Ti:S output.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的目的是进一步发展和保持最先进的快速和超快的光学和红外光谱方法，这些方法对于研究在尽可能广泛的时间尺度上延伸的各种生物过程是不可或缺的。 部分工作旨在开发使用自发辐射作为激发态检测的激发态寿命和各向异性测量的新技术。该方法将用于与Burgess教授合作实验中DNA探针激发态性质的测定。还提出将NOPA装置并入超快荧光上转换装置中，这在DNA标记盒的动态分析中将是特别重要的。例如，在那些研究中，重要的是采用特别调谐到能量供体发色团在490 nm附近的吸收最大值的激发波长，该波长不能通过简单地使用Ti：S输出的二次谐波获得。