DEVELOPMENT OF METHODS OF SINGLE FREQUENCY 2D IR: INFRARED ANALOGUES OF NMR
单频 2D IR 方法的开发:NMR 的红外类似物
基本信息
- 批准号:7598428
- 负责人:
- 金额:$ 11.91万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-09-01 至 2008-05-31
- 项目状态:已结题
- 来源:
- 关键词:AmidesAmino AcidsAspartic AcidBindingBiomedical ResearchClassificationComputer Retrieval of Information on Scientific Projects DatabaseCouplingDevelopmentEnvironmentEnzymesEvaluationFrequenciesFundingGasesGlutamic AcidGoalsGrantHelix (Snails)InstitutionLasersLysineMapsMeasurementMembraneMethodologyMethodsMoldsOpticsPeptidesPhasePhysiologic pulseProceduresPropertyProteinsProtocols documentationPulse takingRangeResearchResearch PersonnelResourcesSecondary Protein StructureSerineShapesSolidSolventsSourceSpectrum AnalysisSpeedStructureTryptophanUnited States National Institutes of HealthWateramyloid peptideanalogbasedetectorear helixmethod developmentmolecular dynamicsresearch studytool
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
There were significant new developments in the femtosecond infrared laser methodology needed for multiple pulse infrared analogues of 2D and 3D-NMR and its application to the dynamics of structures or segments of structures occurring in proteins, enzymes and peptides. It is our continuing aim to mold this methodology into a reliable tool for biomedical research.
This Core is the continuation of the original 2D IR experiments on vibrators associated with peptides. Major goals are:
- Refinement of 2D IR procedures in order to more fully integrate them into biomedical research by advancing and simplifying the methodology of echo spectroscopy to more rapidly expose proximities and other properties of peptide amide groups in water and membrane environments. A triple array detector is proposed that will speed up acquisition by a factor of four.
- Significant improvements of the contrast in the crossed polarization method to facilitate the acquisition of cross peak maps over a broad frequency range that exposes coupling and proximities between peptide modes.
- Development of more robust 2D IR experiment by introducing phase plates, diffractive optics, phase measurement and deformable mirror pulse shaping into the 2D IR apparatus.
- Developments of 2D IR measurements of anharmonicities and their structure sensitivities, angular distributions and couplings representative of protein secondary structures in different solvents and comparisons with those found in gases and molecular dynamics simulations.
- Development of approaches for obtaining structure from 2D IR of C(alpha)-D modes to provide the protocols and theoretical underpinning of the hydrophobic stabilization of transmembrane peptides.
- Systematic evaluation of 2D IR spectra after 13C=18O or 13C=16O replacement of all C=O groups of some small peptides and tryptophan zippers aimed at generating a solid basis for the prediction of amide spectra, the zero order mode frequencies and their delocalization.
- 2D IR and linear IR experiments aimed at structure determination and delocalization of modes in a broad set of examples in different environments including isotopomers of parallel and antiparallel sheets, soluble and membrane bound peptides and helices, aggregates of amyloid peptides and isotopomers of 13C=18O in unusual (non-commercially available as isotopomers) amino acids such as aspartic acid, serine, glutamic acid and lysine.
这个子项目是许多利用
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。所列机构为
研究中心,而研究中心不一定是研究者所在的机构。
2D和3D-NMR的多脉冲红外模拟所需的飞秒红外激光方法及其在蛋白质、酶和肽中发生的结构或结构片段动力学中的应用取得了重大的新进展。我们的目标是将这种方法塑造成生物医学研究的可靠工具。
这个核心是对与肽相关的振动器的原始2D IR实验的延续。主要目标是:
- 改进2D IR程序,以便通过推进和简化回波光谱的方法,更快地暴露肽酰胺基团在水和膜环境中的邻近性和其他性质,将其更充分地整合到生物医学研究中。提出了一种三重阵列探测器,它将使采集速度提高四倍。
- 显著改善交叉极化方法中的对比度,以便于在宽频率范围内采集交叉峰图,从而暴露肽模式之间的耦合和邻近性。
- 通过在二维红外装置中引入相位板、衍射光学、相位测量和变形镜脉冲整形,发展了更鲁棒的二维红外实验。
- 发展二维红外测量的非谐性和它们的结构灵敏度,角分布和耦合代表蛋白质二级结构在不同的溶剂和气体和分子动力学模拟中发现的比较。
- 开发从C(alpha)-D模式的2D IR获得结构的方法,以提供跨膜肽的疏水稳定性的方案和理论基础。
- 系统评价了13 C = 18 O或13 C = 16 O取代某些小肽和色氨酸拉链的所有C=O基团后的二维红外光谱,旨在为预测酰胺光谱、零级模式频率及其离域性提供坚实的基础。
- 2D IR和线性IR实验的目的是在不同环境中的广泛的一组实例中的模式的结构测定和离域,所述实例包括平行和反平行片的同位素异构体、可溶性和膜结合肽和螺旋、淀粉样肽的聚集体和不常见的(非市售的同位素异构体)氨基酸如天冬氨酸、丝氨酸、谷氨酸和赖氨酸中的13 C = 18 O的同位素异构体。
项目成果
期刊论文数量(0)
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{{ truncateString('ROBIN Main HOCHSTRASSER', 18)}}的其他基金
STUDY OF EQUILIBRIUM AND NON-EQUILIBRIUM DYNAMICS BY 2D IR
用二维红外研究平衡和非平衡动力学
- 批准号:
8362565 - 财政年份:2011
- 资助金额:
$ 11.91万 - 项目类别:
2D IR DUAL FREQUENCY AND DUAL ISOTOPE REPLACEMENT STRATEGIES
2D IR 双频和双同位素替代策略
- 批准号:
8362564 - 财政年份:2011
- 资助金额:
$ 11.91万 - 项目类别:
2D IR DUAL FREQUENCY AND DUAL ISOTOPE REPLACEMENT STRATEGIES
2D IR 双频和双同位素替代策略
- 批准号:
8169536 - 财政年份:2010
- 资助金额:
$ 11.91万 - 项目类别:
STUDY OF EQUILIBRIUM AND NON-EQUILIBRIUM DYNAMICS BY 2D IR
用二维红外研究平衡和非平衡动力学
- 批准号:
8169537 - 财政年份:2010
- 资助金额:
$ 11.91万 - 项目类别:
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