METABOLIC FLUX ANALYSIS OF PICHIA STIPITIS

树干毕赤酵母的代谢通量分析

• 批准号: 7598792
• 负责人: Thomas W. Jeffries
• 金额: $ 0.01万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598792
• 关键词: Amino Acids; Carbon; Cells; Computer Retrieval of Information on Scientific Projects Database; Data; Databases; Funding; Gene Targeting; Glucose; Grant; Hydrolysis; Institution; Label; Measures; Metabolic; Metabolism; Pichia; Research; Research Personnel; Resources; Signal Transduction; Simulate; Slice; Source; United States National Institutes of Health; Yeasts; feeding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our objective is to measure the intracellular metabolic flux under steady state; this will provide quantitative information about cell metabolism and allows us to identify possible gene targets for strain improvement. 13C labeled glucose is fed to the cell; yeast cell will be hydrolyzed using 6M HCl and lyophilized. Isotopmer composition of amino acid will be measured using HSQC, in which a slice of strongest carbon signal will be used for integration. The carbon metabolic flux distribution will be simulated by minimizing measured data and simulated data based on input carbon source.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的目标是测量稳态下的细胞内代谢通量，这将提供有关细胞代谢的定量信息，并使我们能够确定菌株改良的可能基因靶点。将13 C标记的葡萄糖进料至细胞;酵母细胞将使用6 M HCl水解并冻干。氨基酸的同位素组成将使用HSQC测量，其中最强碳信号的切片将用于积分。通过最小化实测数据和基于输入碳源的模拟数据来模拟碳代谢通量分布。