VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 我们的目标是确定调节委内瑞拉马脑炎病毒（VEEV）协同组装的分子间相互作用，这是一种在中美洲和南美洲引起流行病的甲病毒，也是一种高度发达的生物武器制剂，但没有有效的治疗方法。目标1。使用互补的生物化学、生物物理学、分子和结构生物学方法，确定VEE病毒衣壳蛋白和RNA之间促进病毒核衣壳组装的相互作用。目标2.通过测定病毒前、病毒和病毒后核衣壳的结构，确定核衣壳内的分子间衣壳蛋白相互作用是否在VEE病毒成熟期间发生大规模重排。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goal is to determine intermolecular interactions that regulate the concerted assembly of Venezuelan equine encephalitis virus (VEEV), an alphavirus that causes epidemics in the Central and South America, and a highly developed biological weapons agent without effective therapy. Aim 1. To determine interactions between the VEE virus capsid protein and RNA that promote viral nucleocapsid assembly, using complementary biochemical, biophysical, molecular and structural biology approaches. Aim 2. To determine if intermolecular capsid protein interactions within the nucleocapsid undergo large-scale rearrangements during VEE virus maturation by determining the structure of pre-viral, viral, and post-viral nucleocapsids.