ARCHITECTURE AND REGULATION OF THE BUDDING YEAST KINETOCHORE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mitotic spindle function critically depends on the kinetochore, a proteinacous structure that serves to connect spindle microtubules to centromeric DNA. While numerous kinetochore proteins have been identified in diverse organisms, how these proteins are organized to connect chromosomes to spindle microtubules, and whether any structural and organizational themes are common to kinetochores of distantly related organisms, are key unanswered questions. Here, we use affinity chromatography and mass spectrometry to generate a map of kinetochore protein interactions. Phosphorylation sites of kinetochore proteins are mapped and the functional consequences of mutating these sites are analyzed in vivo. This will allow a deeper understanding of the architecture and regulation of the budding yeast kinetochore.