SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON

描述：发现了两种新的酵母蛋白Duo282p，An 重要的纺锤体成分，以及Jsn1p，一种似乎影响 微管动力学和富含在微管亲和柱上， 为确定辅助蛋白如何调控提供了独特的机会 体内微管组装的动力学。此外，第三种蛋白质 似乎提供了一种功能上的联系 有丝分裂过程中的微管和肌动蛋白细胞骨架。这种蛋白质，Cor1p 显然是微管和皮质肌动蛋白之间的纽带 纺锤体定向过程中的细胞骨架。这项提议的重点是 研究这些新蛋白质影响的机制 微管动力学和纺锤体功能。具体目标是：(1) 阐明Cor1p与肌动蛋白相互作用的性质和功能 微管细胞骨架及其遗传学和生化鉴定 与酵母Cor1p相互作用的蛋白质；(2)确定特异性 酵母菌Duo282p在纺锤体功能和组装中的作用及测定 其与纺锤体相互作用的性质；(3)阐明Jsn1p在 通过识别具有微管功能的蛋白质来调节微管动力学 或与JSN1p并行。总而言之，这些研究将定义组件 微管和肌动蛋白细胞骨架是确保 适当的染色体遗传。

DESCRIPTION: The discovery of two novel yeast proteins, Duo282p, an essential spindle component, and Jsn1p, a protein that appears to influence microtubule dynamics and is enriched on microtubule affinity columns, provides unique opportunities to determine how accessory proteins regulate the dynamics of microtubule assembly in vivo. In addition, a third protein has been discovered which appears to provide a functional link between the microtubule and actin cytoskeletons during mitosis. This protein, Cor1p apparently serves as a link between microtubules and the cortical actin cytoskeleton during spindle orientation. The focus of this proposal is to investigate the mechanisms by which these novel proteins influence microtubule dynamics and spindle function. The specific aims are to: (1) elucidate the nature and function of Cor1p's interaction with the actin and microtubule cytoskeletons and to genetically and biochemically identify proteins which interact with yeast Cor1p; (2) determine the specific function of yeast Duo282p in spindle function and assembly and determine the nature of its interaction with spindles; (3) elucidate the role of Jsn1p in regulating microtuble dynamics by identifying proteins which function with or in parallel with Jsn1p. In total, these studies will define components of the microtubule and actin cytoskeletons that are required to ensure proper chromosome inheritance.