STU1P FUNCTION IN BUDDING YEAST MITOSIS

STU1P 在芽殖酵母有丝分裂中的功能

• 批准号: 8365860
• 负责人: GEORJANA BARNES
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365860
• 关键词: Binding; Binding Proteins; Biology; Family; Funding; Fungal Genome; Grant; Homologous Gene; Microtubules; Mitosis; Mitotic spindle; National Center for Research Resources; Phosphorylation; Phosphotransferases; Plus End of the Microtubule; Principal Investigator; Protein Kinase; Proteins; Research; Research Infrastructure; Resources; Saccharomyces cerevisiae; Saccharomycetales; Source; Structure; United States National Institutes of Health; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Stu1p is an essential microtubule-binding protein in Saccharomyces cerevisiae, and functions as a regulator of mitotic spindle structure. It is a homolog of the CLASP family of microtubule plus end tracking proteins in metazoans. CLASP family binding partners have been identified in metazoans, but Stu1p's binding partners are unknown. We are trying to determine if Stu1p interacts with other proteins involved in mitosis, particularly in mitotic spindle integrity. Stu1p is also a phospho-protein, but the kinase(s) that phosphorylate it are unknown. We are attempting to identify all phosphorylated residues of Stu1p, as well as determine the kinases responsible for this phosphorylation and if phosphorylation is a regulator of Stu1p function.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 Stu 1 p是酿酒酵母中一种必需的微管结合蛋白，其功能是调节有丝分裂纺锤体结构。它是后生动物中微管加末端跟踪蛋白CLASP家族的同系物。CLASP家族的结合伴侣已经在后生动物中被确定，但Stu 1 p的结合伴侣是未知的。我们正试图确定Stu 1 p是否与其他参与有丝分裂的蛋白质相互作用，特别是在有丝分裂纺锤体完整性方面。Stu 1 p也是一种磷酸化蛋白，但使其磷酸化的激酶尚不清楚。我们正试图确定Stu 1 p的所有磷酸化残基，以及确定负责这种磷酸化的激酶，以及磷酸化是否是Stu 1 p功能的调节剂。