THE KINETOCHORE-MICROTUBULE INTERFACE IN VERTEBRATE CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 有丝分裂细胞面临着将动点连接到生长和缩短微管的挑战性任务，并积极 调节这些动态连接，以产生准确的染色体比对和分离。动毛虫 结合NDC80复合体对于形成和维持强大的动粒-微管连接是必不可少的。我们有 最近发现，它还具有调节动粒-微管+末端动力学和附着的功能 稳定性。微量注射针对Hec1 N-末端特定部位的抗体可抑制两者 脊椎动物细胞运动中心微管脱落和末端组装动力学。我们的结果表明 针对Hec1 N末端的抗体阻止动粒微管的生长或缩短，从而防止 染色体运动和纠错。这里描述的这个项目旨在确定协同作用的共同因素 通过Ndc80复合体来维持对微管的强烈附着并调节这些附着。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mitotic cells face the challenging tasks of linking kinetochores to growing and shortening microtubules and actively regulating these dynamic attachments to produce accurate chromosome alignment and segregation. The kinetochore binding Ndc80 complex is essential for forming and maintaining strong kinetochore-microtubule attachments. We have recently demonstrated that it also has functions in regulating kinetochore-microtubule plus end dynamics and attachment stability. Microinjection of an antibody targeted to a specific site within the N-terminus of Hec1 suppresses both microtubule detachment and plus end assembly dynamics at kinetochores of vertebrate cells. Our results suggest that antibodies to the N-terminus of Hec1 block kinetochore microtubules from growing or shortening, and thereby prevent chromosome movement and error correction. This project described here is designed to identify co-factors that cooperate with the Ndc80 complex to maintain strong attachments to microtubules and regulate these attachments.