CHAR OF GLYCANS FROM MOUSE & BOVINE UROPLAKINS IA & IB BY MASS SPECTROMETRY

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• 批准号: 7601973
• 负责人: Tung-Tien Sun
• 金额: $ 4.31万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601973
• 关键词: Apical; Bacterial Adhesins; Binding; Bos taurus; Carbohydrates; Cattle; Complex; Computer Retrieval of Information on Scientific Projects Database; Digestion; Disease; Endopeptidases; Escherichia coli; Failure; Family; Funding; Gel; Glycoproteins; Glycoside Hydrolases; Grant; Hand; Homologous Gene; Human; Institution; Lectin; Light; Link; Mammals; Mannose; Masks; Mass Spectrum Analysis; Molecular; Mus; Organism; Pathogenesis; Peptide Hydrolases; Polysaccharides; Process; Property; Proteins; Research; Research Personnel; Resources; Site; Source; Structure; Surface; Time; United States National Institutes of Health; Urinary tract infection; Variant; concept; design; glycosylation; human PHEMX protein; inhibitor/antagonist; mannosyl(6)-N-acetyl(2)glucose; mannosyl(9)-N-acetylglucosamine2; member; mimetics; prevent; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although it has been shown that mouse uroplakin (UP) Ia, a major glycoprotein of urothelial apical surface, can serve as the receptor for the FimH lectin adhesin of type 1-fimbriated E. coli, the organism which causes a great majority of urinary tract infections (UTIs), the glycan structure of this native receptor was unknown. Using a sensitive approach that combines in-gel glycosidase and protease digestions, permethylation of released glycans and mass spectrometry, we have elucidated, for the first time, the native glycoform structures of the mouse UPIa receptor and those of its non-binding homolog, UPIb, and have determined the glycosylation site occupancy. UPIa presents a high level of terminally exposed mannose residues (located on Man6GlcNAc2 to Man9GlcNAc2) that are capable of specifically interacting with FimH. We have shown that this property is conserved, not only in the mouse uroplakins, but also in cattle and, even more importantly, in human UPIa, thus establishing the concept that UPIa is a major urothelial receptor in humans and other mammals for the mannose-specific FimH variant. In contrast, our results indicate that most terminally exposed glycans of mouse UPIb are non-mannose residues, thus explaining the failure of FimH to bind to this UPIb. In cattle, on the other hand, complex carbohydrates constituted only about 20% of the UPIb N-linked glycans. Human UPIa contained exclusively high-mannose glycans and human UPIb contained only complex glycans. The drastically different carbohydrate processing of the UPIa and UPIb proteins, two closely related members of the tetraspanin family, may reflect differences in their folding and masking due to their interactions with their associated proteins, UPII and UPIIIa, respectively. Results from this study shed light on the molecular pathogenesis of UTIs and may aid in the design of glyco-mimetic inhibitors for preventing and treating this disease.

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