TOTAL OXIDATIVE POST TRANSLATIONAL MODIFICATION MAP OF H-RAS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目旨在利用我们最近开发的ESI-QQQ-FTMS的独特功能来充分表征在氧化应激条件下发生的H-RAS的翻译后修饰。该项目使用了多种技术的组合，包括自上而下和自下而上的蛋白质组学方法、选择性积累、Q2-CAD和ECD。 所涉及的样品是商业购买的H-RAS，既有天然状态的，也有用过氧亚硝酸根进行氧化修饰的。这最初是在体外完成的，但目标是一旦确定了体外修饰的特征，就将其扩展到体内模型。 一篇关于这项工作的论文发表在《分析化学》杂志上。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aimed to use the unique capabilities of our recently developed ESI-qQq-FTMS to fully characterize the post-translational modifications of H-RAS that occur during oxidative stress conditions. The project used a combination of techniques including top-down and bottom-up proteomics methods, selective accumulation, Q2-CAD, and ECD. The samples involved are commercially purchased H-RAS, both native state and oxidatively modified with peroxynitrite. This was done in-vitro initially, but the goal is to extend this to in-vivo models once the in-vitro modifications are characterized. A paper was published in Analytical Chemistry on this work.