MODEL OF INSP3 DYNAMICS IN DENDRITIC BRANCHLETS OF CEREBELLAR PURKINJE CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 浦肯野神经元突触强度的长期抑制需要肌醇-1，4，5-三磷酸(InsP3)介导的重复钙释放，但InsP3受体在该细胞中高度丰富，对InsP3非常不敏感。突触输入发生在不同的结构上，称为棘突，这些结构是直径约15米的球体，通过狭窄的颈部与树突相连。我们应用了虚拟细胞建模系统来试图解决这个明显的悖论。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Long term depression of synaptic strength in Purkinje neurons requires repeated inositol-1,4,5-trisphosphate (InsP3)-mediated Ca2+ release, but the InsP3 receptor, while highly abundant, is extraordinarily insensitive to InsP3 in this cell. Synaptic inputs occur at distinct structures, called spines, that are ca. 15m diameter spheres connected to the dendrite via a narrow neck. We have applied the Virtual Cell modeling system to try to resolve this apparent paradox.