STRUCTURAL CHARACTERIZATION OF A CHLOROPLAST RIBOSOME

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。高等植物叶绿体含有70年代样的核糖体，根据它们的细菌祖先。然而，这些生物承担着来自多个基因组(核、叶绿体、线粒体)的基因表达协调的负担。此外，叶绿体中基因表达的控制主要是在转录后完成的。我们对叶绿体核糖体的蛋白质组学和结构特征感兴趣，因为这将有助于我们理解该细胞器中存在的独特的翻译控制机制。细胞器核糖体的结构数据还没有发表过。然而，已经发表了对多个叶绿体核糖体的蛋白质组学分析，揭示了标准细菌核糖体独有的保守蛋白质。莱茵衣藻等藻类的叶绿体是大规模蛋白质表达的目标。为了优化各种异源蛋白在这个生物体中的表达水平，我们需要更好地了解翻译调控的作用机制。叶绿体核糖体的结构特征将是这一理解的组成部分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Higher plant chloroplasts contain 70S-like ribosomes, as per their bacterial ancestry. However, these organisms are burdened with the coordination of gene expression from multiple genomes (nuclear, chloroplast, mitochondrial). Additionally, control of gene expression in the chloroplast is accomplished primarily post-transcriptionally. We are interested in a proteomic and structural characterization of the chloroplast ribosome, as this will aid in our understanding of the unique mechanisms of translation control present in this organelle. No structural data from an organelle ribosome has ever been published. However, proteomic analyses have been published from multiple chloroplast ribosomes revealing conserved proteins unique from a standard bacterial ribosome. The chloroplasts of algae like Chlamydomonas reinhardtii are being targeted for large scale protein expression. In order to optimize expression levels of a variety of heterologous proteins in this organism we need to better understand the mechanisms of translation regulation at work. Structural characterization of the chloroplast ribosome will be an integral part of this understanding.