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Cellular Neuroscience Core

细胞神经科学核心

基本信息

批准号：
7699751
负责人：
GABRIEL CORFAS
金额：
$ 19.2万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7699751
关键词：
Acetylcholinesterase Adult Age Alkaline Phosphatase Anatomy Animals Antibodies Antibody Formation Antigens Apoptotic Archives Arts Atlases Autoradiography Avidin Axon Biometry Biotin Boston Brain Bromodeoxyuridine Buffers CDKN1A gene Carmine Cell Death Cell Proliferation Celloidin Cells Chickens Cleaved cell Clinical Clinical Research Cobalt Collaborations Collection Communities Complex Computer software Confocal Microscopy Consultations Coupled DNA Nucleotidylexotransferase DNA-Binding Proteins Dana-Farber Cancer Institute Data Data Analyses Databases Detection Development Digoxigenin Dimethylarsinate Dissection Electron Microscope Electron Microscopy Embryo Emulsions Enzymes Evaluation Experimental Designs Fee-for-Service Plans Film Fixatives Fluorescence Folch-Pi apoprotein Formalin Fostering Freezing Future Galactosidase Gene Expression Generations Genes Genus Capra Germany Glycogen Goat Golgi Apparatus Hematoxylin and Eosin Staining Method Histocytochemistry Histology Horseradish Peroxidase Hospitals Human Human Resources Image Imagery Immunofluorescence Immunologic Immunohistochemistry In Situ In Situ Hybridization Independent Scientist Award Individual Institutes Internet Knowledge Label Laboratories Laboratory Study Link Location Luxol Fast Blue MBS Maps Measurement Mental Retardation and Developmental Disabilities Research Centers Methanol Methods Microscope Microscopy Microtome - medical device Microtomy Mission Molecular Molecular Analysis Monoclonal Antibodies Multienzyme Complexes Mus Myelin Myelin Proteolipid Protein NADP Nerve Tissue Nervous system structure Neuraxis Neuroanatomy Neurons Neurosciences New York Nickel Nucleic Acids Numbers Oryctolagus cuniculus Paraffin Pathologic Pathology Pattern Pediatric Hospitals Peptides Perfusion Plastics Polyesters Preparation Procedures Process Production Proteins Protocols documentation Purpose Pyroxylin RNA Probes Radioactive Reaction Recombinant Proteins Reporter Research Research Personnel Research Project Grants Resolution Retrieval Rodent Saint Jude Children&apos s Research Hospital Sampling Science Scientist Sepharose Services Signal Transduction Silver Staining Slice Slide Specimen Staining method Stains Standards of Weights and Measures Techniques Testing Time Tissue Embedding Tissue Fixation Tissue Sample Tissue Stains Tissues Training Transcript Transgenic Mice Transgenic Organisms Universities Validation Viscosity Waxes Wheat Germ Agglutinins Woman Work araldite base caspase-3 cresyl violet cryostat design diaminobenzidine peroxidase epon experience functional genomics human subject human tissue immunocytochemistry in vivo interest intracellular protein transport investigator training medical schools melting microwave electromagnetic radiation nervous system development neuro-oncology neuropathology oncoprotein p21 paraform programs protein localization location research study sample fixation tissue preparation tool transcription factor uranyl acetate

项目摘要

3.a.2. Overall Objective The overall objective of the Cellular Neuroscience Core is to provide Center investigators access to high quality methods of tissue preparation for state-of-the-art structural and molecular anatomical examination in research projects relevant to the mission of the MRDDRC. Tissues are prepared for standard histology, electron microscopy, confocal microscopy, in situ hybridization, and immunocytochemistry. The Cellular Neuroscience Core also provides for the perfusion of rodents for the purpose of tissue examination and for the design, production and validation of antibodies for immunostaining. A major emphasis of the Core is to provide training for Center investigators and their trainees in the various methods of tissue preparation in order to facilitate the advancement of MRDDRC research. Central to the studies of the normal and abnormal development of the nervous system in humans, as well as in animals, is knowledge of the morphological and topographical features of brain development. For this purpose, the Cellular Neuroscience Core also provides skilled neuropathologists and neuroscientists for consultation. In addition, the Cellular Neuroscience Core provides advice on the use of new online gene expression databases such as Gensat, Functional Genomic Atlas of the Mouse Brain and others. The information available in these databases can, in some instances, help in experimental design and selection of genes of interest for MRDDRC investigators. Finally, because the Core personnel have a wealth of information about the ongoing histology-based projects in the MRDDRC laboratories, the Core fosters collaborations between MRDDRC investigators by linking groups with common interests and complementary expertise and/or tools.

3. a。总体目标