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Cellular Neuroscience Core

细胞神经科学核心

基本信息

批准号：
8137222
负责人：
GABRIEL CORFAS
金额：
$ 21.34万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8137222
关键词：
Acetylcholinesterase Adult Age Alkaline Phosphatase Anatomy Animals Antibodies Antibody Formation Antigens Apoptotic Archives Arts Atlases Autoradiography Avidin Axon Biometry Biotin Boston Brain Bromodeoxyuridine Buffers Carmine Cell Death Cell Proliferation Celloidin Cells Chickens Cleaved cell Clinical Clinical Research Cobalt Collaborations Collection Communities Complex Computer software Confocal Microscopy Consultations Coupled DNA Nucleotidylexotransferase DNA-Binding Proteins Dana-Farber Cancer Institute Data Data Analyses Databases Detection Development Digoxigenin Dimethylarsinate Dissection Electron Microscope Electron Microscopy Embryo Emulsions Enzymes Evaluation Experimental Designs Fee-for-Service Plans Film Fixatives Fluorescence Formalin Fostering Freezing Future Galactosidase Gene Expression Generations Genes Germany Glycogen Goat Golgi Apparatus Hematoxylin and Eosin Staining Method Histocytochemistry Histology Horseradish Peroxidase Hospitals Human Human Resources Image Imagery Immunofluorescence Immunologic Immunohistochemistry In Situ In Situ Hybridization Individual Institutes Internet Knowledge Label Laboratories Laboratory Study Link Location Luxol Fast Blue MBS Maps Measurement Mental Retardation and Developmental Disabilities Research Centers Methanol Methods Microscope Microtome - medical device Microtomy Mission Molecular Molecular Analysis Monoclonal Antibodies Multienzyme Complexes Mus Myelin NADP Nerve Tissue Nervous system structure Neuraxis Neuroanatomy Neurons Neurosciences New York Nickel Nucleic Acids Oryctolagus cuniculus Paraffin Pathologic Pathology Pattern Pediatric Hospitals Peptides Perfusion Plastics Polyesters Preparation Procedures Process Production Proteins Protocols documentation Pyroxylin RNA Probes Radioactive Reaction Recombinant Proteins Reporter Research Research Personnel Research Project Grants Resolution Retrieval Rodent Saint Jude Children&apos s Research Hospital Sampling Science Scientist Sepharose Services Signal Transduction Silver Staining Slice Slide Specimen Staining method Stains Techniques Testing Time Tissue Embedding Tissue Fixation Tissue Sample Tissue Stains Tissues Training Transcript Transgenic Mice Transgenic Organisms Universities Validation Viscosity Waxes Wheat Germ Agglutinins Woman Work araldite base caspase-3 cresyl violet cryostat design diaminobenzidine peroxidase epon experience functional genomics human subject human tissue immunocytochemistry in vivo interest investigator training medical schools melting microwave electromagnetic radiation nervous system development neuro-oncology neuropathology paraform programs research study sample fixation tissue preparation tool transcription factor uranyl acetate

项目摘要

3.a.2. Overall Objective The overall objective of the Cellular Neuroscience Core is to provide Center investigators access to high quality methods of tissue preparation for state-of-the-art structural and molecular anatomical examination in research projects relevant to the mission of the MRDDRC. Tissues are prepared for standard histology, electron microscopy, confocal microscopy, in situ hybridization, and immunocytochemistry. The Cellular Neuroscience Core also provides for the perfusion of rodents for the purpose of tissue examination and for the design, production and validation of antibodies for immunostaining. A major emphasis of the Core is to provide training for Center investigators and their trainees in the various methods of tissue preparation in order to facilitate the advancement of MRDDRC research. Central to the studies of the normal and abnormal development of the nervous system in humans, as well as in animals, is knowledge of the morphological and topographical features of brain development. For this purpose, the Cellular Neuroscience Core also provides skilled neuropathologists and neuroscientists for consultation. In addition, the Cellular Neuroscience Core provides advice on the use of new online gene expression databases such as Gensat, Functional Genomic Atlas of the Mouse Brain and others. The information available in these databases can, in some instances, help in experimental design and selection of genes of interest for MRDDRC investigators. Finally, because the Core personnel have a wealth of information about the ongoing histology-based projects in the MRDDRC laboratories, the Core fosters collaborations between MRDDRC investigators by linking groups with common interests and complementary expertise and/or tools.

3.A.2.总体目标细胞神经科学核心的总体目标是为中心研究人员提供访问权限用于最先进的结构和分子解剖学的高质量组织制备方法在与发展、民主和康复委员会的使命有关的研究项目中进行审查。准备组织，标准组织学、电子显微镜、共聚焦显微镜、原位杂交和免疫细胞化学细胞神经科学核心还提供了啮齿动物的灌注，组织检查的目的以及抗体的设计、生产和验证，免疫染色。核心的一个主要重点是为中心研究者及其培训学员学习各种组织制备方法，以促进MRDDRC的发展 research.神经系统的正常和异常发育的研究中心，人类以及动物的大脑的形态学和地形学特征发展为此，细胞神经科学核心还提供熟练的神经病理学家和神经科学家进行咨询。此外，细胞神经科学核心提供了关于使用新的在线基因表达数据库，如Gensat，小鼠功能基因组图谱大脑和其他。在某些情况下，这些数据库中提供的信息有助于为MRDDRC研究者进行实验设计和选择感兴趣的基因。最后因为核心人员有大量关于正在进行的基于组织学的项目的信息， MRDDRC实验室，核心促进MRDDRC研究人员之间的合作，具有共同利益和互补专长和/或工具的团体。