Cellular Neuroscience Core

3.A.2.总体目标 细胞神经科学核心的总体目标是为中心研究人员提供访问 为最先进的结构和分子解剖学提供高质量的组织准备方法 审查与解除武装、复员和重返社会委员会任务有关的研究项目。组织已经准备好了 常规组织学、电子显微镜、共聚焦显微镜、原位杂交和 免疫细胞化学。细胞神经科学核心还为啮齿类动物提供了 组织检查和抗体的设计、生产和验证的目的 免疫染色。核心的一个主要重点是为中心调查人员和他们的 组织准备的各种方法的受训人员，以促进MRDDRC的进步 研究。主要研究神经系统的正常和异常发育。 人类和动物一样，都是对大脑的形态和地形特征的了解 发展。为此，细胞神经科学核心还提供熟练的神经病理学家 和神经学家进行咨询。此外，细胞神经科学核心提供了关于 使用新的在线基因表达数据库，如Gensat，小鼠功能基因组图谱 大脑和其他人。在某些情况下，这些数据库中的可用信息可以帮助 MRDDRC研究者感兴趣基因的实验设计和选择。最后，因为 核心人员拥有关于正在进行的基于组织学的项目的丰富信息 MRDDRC实验室，核心通过链接促进MRDDRC调查人员之间的合作 具有共同兴趣和互补专业知识和/或工具的团体。

3.a.2. Overall Objective The overall objective of the Cellular Neuroscience Core is to provide Center investigators access to high quality methods of tissue preparation for state-of-the-art structural and molecular anatomical examination in research projects relevant to the mission of the MRDDRC. Tissues are prepared for standard histology, electron microscopy, confocal microscopy, in situ hybridization, and immunocytochemistry. The Cellular Neuroscience Core also provides for the perfusion of rodents for the purpose of tissue examination and for the design, production and validation of antibodies for immunostaining. A major emphasis of the Core is to provide training for Center investigators and their trainees in the various methods of tissue preparation in order to facilitate the advancement of MRDDRC research. Central to the studies of the normal and abnormal development of the nervous system in humans, as well as in animals, is knowledge of the morphological and topographical features of brain development. For this purpose, the Cellular Neuroscience Core also provides skilled neuropathologists and neuroscientists for consultation. In addition, the Cellular Neuroscience Core provides advice on the use of new online gene expression databases such as Gensat, Functional Genomic Atlas of the Mouse Brain and others. The information available in these databases can, in some instances, help in experimental design and selection of genes of interest for MRDDRC investigators. Finally, because the Core personnel have a wealth of information about the ongoing histology-based projects in the MRDDRC laboratories, the Core fosters collaborations between MRDDRC investigators by linking groups with common interests and complementary expertise and/or tools.