MOLECULAR GENETICS, PROTEOMICS AND BIOCHEMICAL ANALYSIS CORE

分子遗传学、蛋白质组学和生化分析核心

• 批准号: 7714622
• 负责人: ERIC P. HOFFMAN
• 金额: $ 22.44万
• 依托单位: CHILDREN'S RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714622
• 关键词: Accounting; Alleles; Arts; Biochemical; Bioinformatics; Biological Assay; Biometry; Biostatistics Core; Biotechnology; Clinical Research Protocols; Collaborations; Computer software; Confidentiality of Patient Information; Consent Forms; Consultations; Critiques; DNA; DNA Sequence; Data; Data Collection; Data Set; Databases; Detection; Experimental Designs; Funding; Gel; Gene Expression; Genetic; Genetic Counseling; Genomics; Genotype; Guns; HCE; High Pressure Liquid Chromatography; Housing; Informatics; Internet; Ions; Label; Liquid Chromatography; Mass Spectrum Analysis; Medical Students; Mental Retardation and Developmental Disabilities Research Centers; Messenger RNA; Methodology; Methods; Molecular; Molecular Biology; Molecular Diagnosis; Molecular Genetics; Molecular Profiling; Mutation; Mutation Detection; Nucleic Acids; Pathway interactions; Peptide Mapping; Pharmaceutical Preparations; Polymerase Chain Reaction; Polymers; Process; Proteins; Proteomics; Quality Control; RNA-Directed DNA Polymerase; Range; Reaction; Recording of previous events; Research; Research Design; Research Ethics Committees; Research Personnel; Research Support; Resources; Reverse Transcriptase Polymerase Chain Reaction; Role; Sampling; Sequence Analysis; Services; Single Nucleotide Polymorphism; Slice; Solutions; Techniques; Testing; Time; Tissue Sample; Training; Validation; Work; base; genetic association; laser capture microdissection; mRNA Expression; method development; response; small molecule; stable isotope; statistics; tandem mass spectrometry; tool

History of Core This core represents a combination of two existing cores: Molecular Genetics and Mass Spectrometry. The Molecular Genetics core was funded in our initial application of 2001. It has been directed since that time by Dr. Eric Hoffman. In 2003, we submitted a supplemental application to establish a mass spectrometry core that had two components, proteomics and biochemical analysis. As the Mass Spectrometry core has developed, it became clear that there was a natural synergy, both from a scientific perspective and physical proximity, between the two cores. In this renewal application we have combined them into a single core, renaming it the Molecular Genetics, Proteomics and Biochemical Analysis Core (MGPBAC). The current director of the mass spectrometry core, Dr. Steven Soldin will assume the role of an Associate Director of the combined core. The basic functions of the two cores continue unchanged. However, in response to the initial critique of the core that suggested enhanced bioinformatics, we have markedly increased this activity as described in the justification section. A. OBJECTIVE The overall objective of the MGPBAC is to provide quantitation of analytes and drug metabolites, stable isotope studies, DMA, mRNA, and proteomics services to MRDDRC investigators. For nucleic acids work, the Core provides basic and advanced molecular biology, molecular genetics, and genetic counseling tools to enhance their clinical research protocols. For proteomics work, the Core provides advanced large molecule mass spectrometry. For biochemical analysis (bioanalytic) work, the core provides tandem mass spectrometry and stable isotope analyses. Specialized database and statistical support is provided for DNA, mRNA and proteomics research. This includes automated genotyping, automated proteomic profiling using stable isotopes, and project-based meta data collection and public dissemination. It is important to note that the database and statistical support is distinct from that offered in the Biostatistics and Informatics Core. The statistics and bioinformatics offered by the MGPBAC is not available in the Biostatistics and Informatics Core, and is highly specialized for molecular biology work and research. However, there is a linkage between the two cores to assure synergy.

核心历史 该核心代表了两个现有核心的组合：分子遗传学和质谱。的 分子遗传学核心在我们2001年的最初申请中得到资助。从那时起， 博士埃里克霍夫曼。2003年，我们提交了一份补充申请， 它有两个组成部分，蛋白质组学和生化分析。因为质谱仪的核心 发展，很明显，有一个自然的协同作用，无论是从科学的角度和物理 两个核心之间的距离。在这个更新应用程序中，我们将它们合并为一个核心， 分子遗传学、蛋白质组学和生物化学分析核心（MGPBAC）。当前 质谱核心主任Steven Soldin博士将担任副主任 组合核心两个核心的基本功能继续保持不变。然而，为了应对最初的 在对建议增强生物信息学的核心的批评之后，我们显著增加了这一活动， 在理由部分中描述。 A.目的 MGPBAC的总体目标是提供分析物和药物代谢物的定量，稳定 为MRDDRC研究人员提供同位素研究、DMA、mRNA和蛋白质组学服务。对于核酸工作， 核心提供基础和先进的分子生物学，分子遗传学和遗传咨询工具， 加强他们的临床研究方案。对于蛋白质组学工作，核心提供先进的大分子 质谱分析法来对于生化分析（生物分析）工作，核心提供串联质谱 稳定同位素分析。为DNA、mRNA和DNA序列提供了专门的数据库和统计支持。 蛋白质组学研究。这包括自动化基因分型，使用稳定的 同位素以及基于项目的Meta数据收集和公共传播。需要注意的是， 数据库和统计支持与生物统计学和信息学核心所提供的支持不同。的 统计学和生物信息学提供的MGPBAC是不可用的生物统计学和信息学核心， 并高度专业化的分子生物学工作和研究。然而，在这两者之间存在着联系。 两个核心以确保协同作用。