MOLECULAR GENETICS, PROTEOMICS AND BIOCHEMICAL ANALYSIS CORE

核心的历史 这个核心代表了两个现有核心的组合：分子遗传学和质谱学。这个 分子遗传学核心是在我们2001年的最初申请中获得资助的。从那时起，它的导演就是 埃里克·霍夫曼医生。2003年，我们提交了一份补充申请，要求建立一个质谱学核心 它有两个组成部分，蛋白质组学和生化分析。正如质谱学核心所拥有的 发展起来，很明显，无论是从科学角度还是从物理角度来看，都存在一种自然的协同效应 两个核心之间的接近。在此续订申请中，我们将它们合并到一个核心中， 更名为分子遗传学、蛋白质组学和生化分析核心(MGPBAC)。海流 质谱学核心主任史蒂文·索尔丁博士将担任 组合式磁芯。两个核心的基本功能保持不变。然而，为了回应最初的 对建议增强生物信息学的核心的批评，我们显著增加了这一活动，因为 在对齐部分中进行了说明。 A.目标 MGPBAC的总体目标是提供分析物和药物代谢物的定量，稳定 向MRDDRC研究人员提供同位素研究、DNA、信使核糖核酸和蛋白质组学服务。对于核酸的工作， CORE提供基础和高级分子生物学、分子遗传学和遗传咨询工具 加强他们的临床研究方案。对于蛋白质组学工作，Core提供先进的大分子 质谱学。对于生化分析(生物分析)工作，核心提供串联质谱仪 和稳定同位素分析。为DNA、信使核糖核酸和 蛋白质组学研究。这包括自动基因分型、使用STRATE的自动蛋白质组分析 同位素，以及基于项目的元数据收集和公开传播。需要注意的是， 数据库和统计支持有别于生物统计和信息学核心中提供的支持。这个 MGPBAC提供的统计和生物信息学在生物统计和信息学核心中不可用， 并高度专业化地从事分子生物学工作和研究。然而，两者之间存在着联系 两个核心以确保协同。

History of Core This core represents a combination of two existing cores: Molecular Genetics and Mass Spectrometry. The Molecular Genetics core was funded in our initial application of 2001. It has been directed since that time by Dr. Eric Hoffman. In 2003, we submitted a supplemental application to establish a mass spectrometry core that had two components, proteomics and biochemical analysis. As the Mass Spectrometry core has developed, it became clear that there was a natural synergy, both from a scientific perspective and physical proximity, between the two cores. In this renewal application we have combined them into a single core, renaming it the Molecular Genetics, Proteomics and Biochemical Analysis Core (MGPBAC). The current director of the mass spectrometry core, Dr. Steven Soldin will assume the role of an Associate Director of the combined core. The basic functions of the two cores continue unchanged. However, in response to the initial critique of the core that suggested enhanced bioinformatics, we have markedly increased this activity as described in the justification section. A. OBJECTIVE The overall objective of the MGPBAC is to provide quantitation of analytes and drug metabolites, stable isotope studies, DMA, mRNA, and proteomics services to MRDDRC investigators. For nucleic acids work, the Core provides basic and advanced molecular biology, molecular genetics, and genetic counseling tools to enhance their clinical research protocols. For proteomics work, the Core provides advanced large molecule mass spectrometry. For biochemical analysis (bioanalytic) work, the core provides tandem mass spectrometry and stable isotope analyses. Specialized database and statistical support is provided for DNA, mRNA and proteomics research. This includes automated genotyping, automated proteomic profiling using stable isotopes, and project-based meta data collection and public dissemination. It is important to note that the database and statistical support is distinct from that offered in the Biostatistics and Informatics Core. The statistics and bioinformatics offered by the MGPBAC is not available in the Biostatistics and Informatics Core, and is highly specialized for molecular biology work and research. However, there is a linkage between the two cores to assure synergy.