MOLECULAR GENETICS, PROTEOMICS AND BIOCHEMICAL ANALYSIS CORE

点击翻译按钮获取中文摘要

History of Core This core represents a combination of two existing cores: Molecular Genetics and Mass Spectrometry. The Molecular Genetics core was funded in our initial application of 2001. It has been directed since that time by Dr. Eric Hoffman. In 2003, we submitted a supplemental application to establish a mass spectrometry core that had two components, proteomics and biochemical analysis. As the Mass Spectrometry core has developed, it became clear that there was a natural synergy, both from a scientific perspective and physical proximity, between the two cores. In this renewal application we have combined them into a single core, renaming it the Molecular Genetics, Proteomics and Biochemical Analysis Core (MGPBAC). The current director of the mass spectrometry core, Dr. Steven Soldin will assume the role of an Associate Director of the combined core. The basic functions of the two cores continue unchanged. However, in response to the initial critique of the core that suggested enhanced bioinformatics, we have markedly increased this activity as described in the justification section. A. OBJECTIVE The overall objective of the MGPBAC is to provide quantitation of analytes and drug metabolites, stable isotope studies, DMA, mRNA, and proteomics services to MRDDRC investigators. For nucleic acids work, the Core provides basic and advanced molecular biology, molecular genetics, and genetic counseling tools to enhance their clinical research protocols. For proteomics work, the Core provides advanced large molecule mass spectrometry. For biochemical analysis (bioanalytic) work, the core provides tandem mass spectrometry and stable isotope analyses. Specialized database and statistical support is provided for DNA, mRNA and proteomics research. This includes automated genotyping, automated proteomic profiling using stable isotopes, and project-based meta data collection and public dissemination. It is important to note that the database and statistical support is distinct from that offered in the Biostatistics and Informatics Core. The statistics and bioinformatics offered by the MGPBAC is not available in the Biostatistics and Informatics Core, and is highly specialized for molecular biology work and research. However, there is a linkage between the two cores to assure synergy.