BIOCHEMICAL STUDIES OF BLOOD COAGULATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在这项研究中，PI将检查导致出血或血栓形成的凝血特征。她将检查血细胞的促凝血活性。她将使用一种利用刺激单眼的TF启动的凝血模型系统来进行这些研究。新鲜分离的血小板和纯化的凝血蛋白以及凝血酶生成、血小板活化和纤维蛋白凝块结构的相关参数。将从受试者手臂的静脉中抽取5至50毫升的血液。单核细胞和血小板将从血液中分离出来，用于随后的体外实验。检测体外各种条件下单核细胞的活性、血小板的活化和凝血酶的生成。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study, the PI will examine features of coagulation that lead to bleeding or thrombosis. She will examine procoagulant activity of blood cells. She will perform these studies using a TF-initiated model system of coagulation that utilized stimulated monoctyes. freshly isolated platelets and purified coagulation proteins and correlate parameters of thrombin generation, platelet activation and fibrin clot structure. Five to 50 ml of blood will be drawn from a vein in the subject's arm. Monocytes and platelets will be isolated from the blood and used in subsequent in vitro experiments. The activity of monocytes, platelet activation and thrombin generation under a variety of conditions in vitro will be examined.