BIOCHEMICAL STUDIES OF BLOOD COAGULATION

凝血的生物化学研究

• 批准号: 7625665
• 负责人: Alisa S. Wolberg
• 金额: $ 0.04万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7625665
• 关键词: Biochemical; Biological Models; Blood; Blood Cells; Blood Platelets; Blood coagulation; Coagulation Process; Computer Retrieval of Information on Scientific Projects Database; Condition; Fibrin; Funding; Generations; Grant; Hemorrhage; In Vitro; Institution; Lead; Platelet Activation; Proteins; Research; Research Personnel; Resources; Source; Structure; Thrombin; Thrombosis; United States National Institutes of Health; Upper arm; Veins; monocyte; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study, the PI will examine features of coagulation that lead to bleeding or thrombosis. She will examine procoagulant activity of blood cells. She will perform these studies using a TF-initiated model system of coagulation that utilized stimulated monoctyes. freshly isolated platelets and purified coagulation proteins and correlate parameters of thrombin generation, platelet activation and fibrin clot structure. Five to 50 ml of blood will be drawn from a vein in the subject's arm. Monocytes and platelets will be isolated from the blood and used in subsequent in vitro experiments. The activity of monocytes, platelet activation and thrombin generation under a variety of conditions in vitro will be examined.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在本研究中，PI将检查导致出血或血栓形成的凝血特征。 她将检查血细胞的促凝活性。 她将使用TF启动的凝血模型系统进行这些研究，该系统利用刺激的单核细胞。新鲜分离的血小板和纯化的凝血蛋白以及凝血酶生成、血小板活化和纤维蛋白凝块结构的相关参数。 将从受试者手臂静脉抽取5 - 50 ml血液，从血液中分离单核细胞和血小板，并用于随后的体外实验。 将在各种体外条件下检查单核细胞活性、血小板活化和凝血酶生成。