COBRE: MUSC: P1: EPIDEMIOL ORAL DIS & DIABETES: CYTOKINE GENES & INFLAMMATION

COBRE：MUSC：P1：流行性口腔疾病

• 批准号: 7610828
• 负责人: JOYCE J FERNANDES
• 金额: $ 17.89万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610828
• 关键词: Admixture; Adolescent; Adult; African American; Age; C-reactive protein; CCL2 gene; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Cytokine Gene; Data; Dental; Diabetes Mellitus; Epidemiologic Studies; Funding; Gelatinase A; Gelatinase B; Gender; Gene Frequency; Genes; Genetic Polymorphism; Genotype; Gingiva; Gingivitis; Glycosylated Hemoglobin; Glycosylated hemoglobin A; Grant; IL8 gene; Inflammation; Institution; Interleukin-1; Interleukin-10; Interleukin-6; Interstitial Collagenase; Life; Liquid substance; Matrix Metalloproteinases; Medical; Modeling; Neutrophil Collagenase; Non-Insulin-Dependent Diabetes Mellitus; Oral; Oral health; Periodontal Diseases; Periodontitis; Polymerase Chain Reaction; Population; Prevalence; Research; Research Personnel; Resources; Severities; Source; South Carolina; Statistical Models; Stromelysin 1; Techniques; Testing; Tissue Inhibitor of Metalloproteinase-1; United States National Institutes of Health; cigarette smoking; collagenase; collagenase 3; cytokine; glycemic control; health practice

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Our overlying hypothesis is that better glycemic control (glycated hemoglobin A1c, or A1c) in diabetes is associated with less prevalent and less severe periodontal disease (gingivitis and periodontitis) and that this association is dependent on genotype ofcytokine genes (IL-IB+3953,IL-10-1082,TNF-a-308, and TNF-a-238), cytokine levels in gingival cervicular fluid and C-reactive protein levels. We will conduct an epidemiological study by obtaining data from cross-sectional medical and dental assessment of Gullah-speaking adolescent and adult African Americans with type 2 diabetes living on the coast of the South Carolina .This population is unique because of minimal gentic admixture Specific Aim 1: To test the hypothesis that cytokine gene polymorphism is associated with periodontal disease in African Americans with diabetes. We will determine polymorphism of key candidate cytokine genes by polymerase chain reaction (PCR) technique and assess periodontal disease. We will describe allele frequency of cytokine gene polymorphisms in association with prevalence and severity of periodontal disease. Specific Aim 2: To test the hypothesis that levels of cytokines IL-1¿, IL-1, IL-6, IL-8, IL-10, INFg, TNFa, MCP-1, MMP-1, MMP-2, MMP-3, MMP-1, MMP-8, MMP-9, MMP-13, TIMP-1, TIMP-2, and TGF¿ and levels of C-reactive protein (CRP) are correlated to prevalence and severity of periodontal disease in African Americans with diabetes. Specific Aim 3: To test the hypothesis that better diabetes glycemic control is associated with reduced levels of cytokines, MMPs, and C-reactive protein and with increase TIMP-1 and collagenase activity, which is associated to reduced prevalence and severity of periodontal disease. We will employ statistical models to assess interrelationship between Ale and cytokine levels, between Ale and MMPand TIMP and between Ale and C-reactive protein levels. In modeling the relationship, we will take consideration of periodontal disease, oral health practice, age, gender, cigarette smoking, and duration of diabetes.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 描述（申请人提供）：我们的假设是更好的血糖控制糖尿病患者的糖化血红蛋白A1 c（糖化血红蛋白A1 c，或A1 c）与牙周病的患病率和严重程度较低有关（牙龈炎和牙周炎），这种关联依赖于细胞因子基因的基因型（IL-1B +3953、IL-10-1082、TNF-α-308和TNF-α-238）、牙龈颈液中的细胞因子水平和C-反应蛋白水平。我们将通过对居住在南卡罗来纳州海岸的、讲Gullah语的青少年和成年2型糖尿病非裔美国人进行横断面医学和牙科评估来获得数据，从而进行一项流行病学研究。我们将通过聚合酶链反应（PCR）技术确定关键候选细胞因子基因的多态性，并评估牙周疾病。我们将描述细胞因子基因多态性的等位基因频率与牙周病的患病率和严重程度。 具体目标二：检验细胞因子IL-1 '、IL-1、IL-6、IL-8、IL-10、INFg、TNFa、MCP-1、MMP-1、MMP-2、MMP-3、MMP-1、MMP-8、MMP-9、MMP-13、TIMP-1、TIMP-2和TGF'水平以及C反应蛋白（CRP）水平与非裔美国糖尿病患者牙周病的患病率和严重程度相关的假设。具体目标3：为了验证更好的糖尿病血糖控制与细胞因子、MMPs和C反应蛋白水平降低以及TIMP-1和胶原酶活性增加相关的假设，这与牙周病的患病率和严重程度降低相关。 我们将采用统计模型来评估Ale与细胞因子水平之间、Ale与MMP和TIMP之间以及Ale与C-反应蛋白水平之间的相互关系。在建立这种关系的模型时，我们将考虑牙周疾病、口腔健康实践、年龄、性别、吸烟和糖尿病持续时间。