COBRE: MUSC: P1: EPIDEMIOL ORAL DIS & DIABETES: CYTOKINE GENES & INFLAMMATION

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Our overlying hypothesis is that better glycemic control (glycated hemoglobin A1c, or A1c) in diabetes is associated with less prevalent and less severe periodontal disease (gingivitis and periodontitis) and that this association is dependent on genotype ofcytokine genes (IL-IB+3953,IL-10-1082,TNF-a-308, and TNF-a-238), cytokine levels in gingival cervicular fluid and C-reactive protein levels. We will conduct an epidemiological study by obtaining data from cross-sectional medical and dental assessment of Gullah-speaking adolescent and adult African Americans with type 2 diabetes living on the coast of the South Carolina .This population is unique because of minimal gentic admixture Specific Aim 1: To test the hypothesis that cytokine gene polymorphism is associated with periodontal disease in African Americans with diabetes. We will determine polymorphism of key candidate cytokine genes by polymerase chain reaction (PCR) technique and assess periodontal disease. We will describe allele frequency of cytokine gene polymorphisms in association with prevalence and severity of periodontal disease. Specific Aim 2: To test the hypothesis that levels of cytokines IL-1¿, IL-1, IL-6, IL-8, IL-10, INFg, TNFa, MCP-1, MMP-1, MMP-2, MMP-3, MMP-1, MMP-8, MMP-9, MMP-13, TIMP-1, TIMP-2, and TGF¿ and levels of C-reactive protein (CRP) are correlated to prevalence and severity of periodontal disease in African Americans with diabetes. Specific Aim 3: To test the hypothesis that better diabetes glycemic control is associated with reduced levels of cytokines, MMPs, and C-reactive protein and with increase TIMP-1 and collagenase activity, which is associated to reduced prevalence and severity of periodontal disease. We will employ statistical models to assess interrelationship between Ale and cytokine levels, between Ale and MMPand TIMP and between Ale and C-reactive protein levels. In modeling the relationship, we will take consideration of periodontal disease, oral health practice, age, gender, cigarette smoking, and duration of diabetes.