COBRE: MUSC: P1: EPIDEMIOL ORAL DIS & DIABETES: CYTOKINE GENES & INFLAMMATION

COBRE：MUSC：P1：流行性口腔疾病

• 批准号: 7381880
• 负责人: JOYCE J FERNANDES
• 金额: $ 12.15万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381880
• 关键词: COBRE; MUSC; P1; EPIDEMIOL; ORAL

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Our overlying hypothesis is that better glycemic control (glycated hemoglobin A1c, or A1c) in diabetes is associated with less prevalent and less severe periodontal disease (gingivitis and periodontitis) and that this association is dependent on genotype ofcytokine genes (IL-IB+3953,IL-10-1082,TNF-a-308, and TNF-a-238), cytokine levels in gingival cervicular fluid and C-reactive protein levels. We will conduct an epidemiological study by obtaining data from cross-sectional medical and dental assessment of Gullah-speaking adolescent and adult African Americans with type 2 diabetes living on the coast of the South Carolina .This population is unique because of minimal gentic admixture Specific Aim 1: To test the hypothesis that cytokine gene polymorphism is associated with periodontal disease in African Americans with diabetes. We will determine polymorphism of key candidate cytokine genes by polymerase chain reaction (PCR) technique and assess periodontal disease. We will describe allele frequency of cytokine gene polymorphisms in association with prevalence and severity of periodontal disease. Specific Aim 2: To test the hypothesis that levels of cytokines IL-1¿, IL-1, IL-6, IL-8, IL-10, INFg, TNFa, MCP-1, MMP-1, MMP-2, MMP-3, MMP-1, MMP-8, MMP-9, MMP-13, TIMP-1, TIMP-2, and TGF¿ and levels of C-reactive protein (CRP) are correlated to prevalence and severity of periodontal disease in African Americans with diabetes. Specific Aim 3: To test the hypothesis that better diabetes glycemic control is associated with reduced levels of cytokines, MMPs, and C-reactive protein and with increase TIMP-1 and collagenase activity, which is associated to reduced prevalence and severity of periodontal disease. We will employ statistical models to assess interrelationship between Ale and cytokine levels, between Ale and MMPand TIMP and between Ale and C-reactive protein levels. In modeling the relationship, we will take consideration of periodontal disease, oral health practice, age, gender, cigarette smoking, and duration of diabetes.

该主题是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是针对该中心的，这不是调查人员的机构。描述（由适用提供）：我们上覆的假设是，糖尿病中更好的糖尿病控制（糖糖蛋白A1C或A1C）与较少且严重严重的牙周疾病（牙龈炎和牙周炎）有关，并且该关联依赖于细胞因子基因的基因型（IL-ib-ib-ib+3953，IL-3953，IIL，IL-ir-il-ir-ir，IL-ir-ir-ir，和TNF-A-238），牙龈宫颈液和C反应蛋白水平的细胞因子水平。我们将通过从居住在南卡罗来纳州海岸上的2型糖尿病的青少年和成年非裔美国人的横截面医学和牙科评估中获得数据进行流行病学研究。该人群是独一无二的。由于绅士的特定目的1：测试了与疾病的疾病相关的假说，这是由于最少的特定目标。我们将通过聚合酶链反应（PCR）技术和评估牙周疾病来确定关键候选细胞因子基因的多态性。我们将描述细胞因子基因多态性的等位基因频率与牙周疾病的患病率和严重程度有关。具体目的2：测试细胞因子IL-1，IL-1，IL-6，IL-8，IL-10，INFG，TNFA，MCP-1，MMP-1，MMP-1，MMP-1，MMP-3，MMP-1，MMP-1，MMP-1，MMP-8，MMP-9，MMP-13，MMP-13，MMP-13，TIMP-1，TIMP-2和TGF。 （CRP）与患有糖尿病的非洲裔美国人的牙周疾病的患病率和严重程度相关。具体目的3：检验以下假设：更好的糖尿病血糖控制与细胞因子，MMP和C反应蛋白的水平降低有关，以及TIMP-1和胶原酶活性的增加，这与牙周疾病的患病率和严重程度降低有关。我们将采用统计模型来评估ALE和MMPAND TIMP之间以及ALE和C反应性蛋白水平之间的ALE和细胞因子水平之间的相互关系。在建模关系时，我们将考虑牙周疾病，口腔健康实践，年龄，性别，吸烟和糖尿病持续时间。