P2: MYOGENIC POTENTIAL OF BONE MARROW CELLS
P2:骨髓细胞的生肌潜能
基本信息
- 批准号:7610567
- 负责人:
- 金额:$ 37.45万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-05-01 至 2008-04-30
- 项目状态:已结题
- 来源:
- 关键词:Bone Marrow CellsCell CountCell TransplantsCellsCharacteristicsComputer Retrieval of Information on Scientific Projects DatabaseDesminDirect Lytic FactorsEventFrequenciesFundingGrantGreen Fluorescent ProteinsInfusion proceduresInjection of therapeutic agentInjuryInstitutionLeadMarrowMusMuscleMuscle CellsMuscle FibersNatureNumbersPopulationRadiationRateResearchResearch PersonnelResourcesSkeletal muscle injurySourceTimeTransplantationTreatment ProtocolsUnited States National Institutes of Healthcell typein vivotransdifferentiation
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Conversion of green-fluorescent protein (GFP) positive marrow cells to skeletal muscle cells has been demonstrated and is the focus of this project. We have determined that donor GFP+ marrow cells have converted to muscle cells by colocalizing GFP and desmin in morphologically characteristic muscle fibers. We have found increasing levels of marrow conversion or transdifferentiation to skeletal muscle cells by altering the specifics of the transplant regimen including cell number, timing of transplant and mode of cell delivery (i.e. local injection vs systemic infusion or mobilization of transplanted cells). We have also found that the number of conversion events changes with different mobilization regimens and that subsets of marrow cells, give higher rates of conversion than unseparated marrow cells. The nature of the skeletal muscle injury, radiation or direct cardiotoxin injection, is also critical in determining the level of transdifferentiation of marrow to muscle cells seen in vivo. In preliminary studies using a cardiotoxin muscle injury in previously transplanted mice, combined with radiation and direct injection of different populations of marrow cells we have obtained conversion rates up to12%, i.e. 12% of the muscle fibers were GFP+ skeletal muscle cells. This project evaluates the specifics of muscle injury which will lead to high-level conversion of marrow to muscle and to explore which particular cell type can give rise to muscle at the highest frequency, the timing of transplant suitable for such conversions and the number of cells necessary to obtain significant muscle conversion.
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。列出的机构为
中心,但不一定是研究者所在的机构。
绿色荧光蛋白(GFP)阳性骨髓细胞转化为骨骼肌细胞已被证明,是这个项目的重点。我们已经确定,供体GFP+骨髓细胞已转化为肌细胞共定位的GFP和结蛋白在形态特征的肌纤维。我们发现,通过改变移植方案的具体细节,包括细胞数量、移植时间和细胞输送方式(即局部注射与全身输注或移植细胞动员),骨髓转化或转分化为骨骼肌细胞的水平会增加。我们还发现,转换事件的数量随着不同的动员方案而变化,并且骨髓细胞的亚群比未分离的骨髓细胞具有更高的转换率。骨骼肌损伤、辐射或直接心脏毒素注射的性质在确定体内骨髓向肌细胞转分化的水平方面也是至关重要的。 在先前移植的小鼠中使用心脏毒素肌肉损伤的初步研究中,结合辐射和直接注射不同群体的骨髓细胞,我们已经获得了高达12%的转化率,即12%的肌纤维是GFP+骨骼肌细胞。该项目评估了肌肉损伤的具体情况,这将导致骨髓向肌肉的高水平转化,并探索哪种特定的细胞类型可以以最高频率产生肌肉,适合这种转化的移植时机以及获得显着肌肉转化所需的细胞数量。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MEHRDAD ABEDI其他文献
MEHRDAD ABEDI的其他文献
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{{ truncateString('MEHRDAD ABEDI', 18)}}的其他基金
BONE MARROW DERIVED FIBROBLASTS AND THEIR ROLE IN TISSUE REPAIR AND FIBROSIS
骨髓来源的成纤维细胞及其在组织修复和纤维化中的作用
- 批准号:
8167640 - 财政年份:2010
- 资助金额:
$ 37.45万 - 项目类别:
BONE MARROW DERIVED FIBROBLASTS AND THEIR ROLE IN TISSUE REPAIR AND FIBROSIS
骨髓来源的成纤维细胞及其在组织修复和纤维化中的作用
- 批准号:
7959648 - 财政年份:2009
- 资助金额:
$ 37.45万 - 项目类别:
COBRE: RW HOSP: P2 MYOGENIC POTENTIAL OF BONE MARROW CELLS
COBRE:RW HOSP:骨髓细胞的 P2 生肌潜能
- 批准号:
7382033 - 财政年份:2006
- 资助金额:
$ 37.45万 - 项目类别:
COBRE: RW HOSP: P2 MYOGENIC POTENTIAL OF BONE MARROW CELLS
COBRE:RW HOSP:骨髓细胞的 P2 生肌潜能
- 批准号:
7171262 - 财政年份:2005
- 资助金额:
$ 37.45万 - 项目类别:
Cell Cycle Related Transdifferentation Plasticity
细胞周期相关的转分化可塑性
- 批准号:
6775588 - 财政年份:2003
- 资助金额:
$ 37.45万 - 项目类别:
Cell Cycle Related Transdifferentation Plasticity
细胞周期相关的转分化可塑性
- 批准号:
6677573 - 财政年份:2003
- 资助金额:
$ 37.45万 - 项目类别:
Cell Cycle Related Transdifferentation Plasticity
细胞周期相关的转分化可塑性
- 批准号:
7084635 - 财政年份:2003
- 资助金额:
$ 37.45万 - 项目类别:
Cell Cycle Related Transdifferentation Plasticity
细胞周期相关的转分化可塑性
- 批准号:
6915569 - 财政年份:2003
- 资助金额:
$ 37.45万 - 项目类别:
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