Impacts of Benzo[a]pyrene on Microbiome Development across Lifespan and Generations and the Behavioral Consequences

苯并[a]芘对整个生命周期和几代人微生物组发育的影响以及行为后果

• 批准号: 10544462
• 负责人: Thomas Jefferson Sharpton
• 金额: $ 6.23万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10544462
• 关键词: Active Learning; Adolescent; Affect; Applications Grants; Area; Attention; Award; Behavior; Behavioral; Benzo(a)pyrene; Bioinformatics; Biological; Biological Models; Chemicals; Communities; Data Science; Development; Development Plans; Educational workshop; Embryo; Enrollment; Ensure; Environmental Pollutants; Exposure to; Fishes; Food Contamination; Funding; Generations; Gnotobiotic; Goals; Grant; Human; Human Resources; Individual; Infrastructure; Interdisciplinary Study; Lead; Longevity; Manuscripts; Measures; Mediating; Mentors; Mentorship; Modality; Modeling; Molecular; National Institute of Environmental Health Sciences; Neurodevelopmental Impairment; Oral cavity; Oregon; Outcome; Parents; Poly-fluoroalkyl substances; Publishing Peer Reviews; Research; Research Project Grants; Research Training; Role; Science; Scientist; Series; Stretching; Systems Biology; Techniques; Technology; Toxicology; Training; Training Programs; Training Support; Underrepresented Students; Universities; Vertebrates; Veterans; Work; Writing; Zebrafish; analytical tool; base; behavioral impairment; biocomputing; bioinformatics resource; biomedical scientist; black women; career; career networking; contaminated water; doctoral student; early life exposure; environmental chemical; experience; experimental study; follow-up; graduate student; gut microbiome; improved; in vivo; interest; meetings; member; microbiome; microbiota; multidimensional data; neurodevelopment; neurodevelopmental effect; neurotoxicity; peer; pollutant; programs; skills; success; symposium; technology/technique; theories; tool; toxicant

PROJECT SUMMARY/ABSTRACT This supplement grant application aims to support the professional development of a Black, female, veteran graduate student who is pursuing a career as a toxicology research scientist. To accomplish this goal, the candidate’s co-mentors – Dr. Thomas Sharpton and Dr. Robyn Tanguay – have worked with the candidate to develop a research, training, and mentorship plan that will ensure the candidate’s professional success. This plan includes an extensive interdisciplinary training program that incorporates both didactic and experiential learning modalities, as well as a personalized, intimate, and supportive mentorship program. Additionally, the plan includes an interdisciplinary and cutting-edge research project that will help the candidate catapult their career forward. In particular, the candidate will conduct research that extends the R01 parent award into complimentary, but new areas. Briefly, the parent award focuses on defining how early life exposure to pollutants impacts assembly of the gut microbiome to impair behavior development, and focuses specifically on benzo[a]pyrene as a model pollutant. As part of this supplement grant, the candidate will apply the tools and technologies developed under the parent award to investigate a specific class of environmental chemicals of concern, namely a diverse array of per- and polyfluoroalkyl substances (PFAS) that have been shown to impair behavior development in vertebrates. In so doing, the candidate will resolve how embryonic exposure to various PFAS impacts gut microbiome assembly and whether these alterations to the gut microbiome mediate how PFAS impair behavior. As a result, the candidate will obtain interdisciplinary experience in an emerging area of toxicology, especially with respect to bioinformatic and data science approaches, and will clarify potential mechanisms through which PFAS elicit their effect on vertebrate behavior. In total, the proposed plan will equip a passionate junior scientist from an underrepresented community to thrive in the competitive research landscape.

项目摘要/摘要 这项补充赠款申请旨在支持黑人，女性，退伍军人的专业发展 从事毒理学研究科学家职业的研究生。为了实现这一目标， 候选人的联合官员 - 托马斯·夏普顿（Thomas Sharpton）博士和罗宾·坦格（Robyn Tanguay）博士 - 与候选人合作 制定一项研究，培训和心态计划，以确保候选人的职业成功。 计划包括一项广泛的跨学科培训计划，该计划既结合了教学和经验丰富 学习方式以及个性化，亲密和支持的心态计划。另外， 计划包括一个跨学科和尖端的研究项目，该项目将帮助候选人弹射 职业前进。特别是，候选人将进行将R01父母奖扩展到的研究 免费，但新领域。简而言之，父母奖的重点是确定早期寿命如何暴露 污染物会影响肠道微生物组的组装以损害行为的发展，并专门针对 苯并[a] pyrene作为模型污染物。作为这项补充赠款的一部分，候选人将应用工具和 根据父母奖开发的技术，以调查特定类别的环境化学品 关注者，即已显示损害的潜水员和多氟烷基物质（PFA） 脊椎动物的行为发展。这样做，候选人将解决胚胎暴露于 各种PFA会影响肠道微生物组的组装，以及这些对肠道微生物组的改变是否介导 PFA如何损害行为。结果，候选人将获得新兴的跨学科经验 毒理学领域，尤其是关于生物信息学和数据科学方法的领域，并将澄清 PFA引起其对脊椎动物行为的影响的潜在机制。总共提议的计划 将为一位充满激情的初级科学家提供从代表性不足的社区中蓬勃发展的初级科学家 研究格局。