Impacts of Benzo[a]pyrene on Microbiome Development across Lifespan and Generations and the Behavioral Consequences

苯并[a]芘对整个生命周期和几代人微生物组发育的影响以及行为后果

• 批准号: 10544462
• 负责人: Thomas Jefferson Sharpton
• 金额: $ 6.23万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10544462
• 关键词: Active Learning; Adolescent; Affect; Applications Grants; Area; Attention; Award; Behavior; Behavioral; Benzo(a)pyrene; Bioinformatics; Biological; Biological Models; Chemicals; Communities; Data Science; Development; Development Plans; Educational workshop; Embryo; Enrollment; Ensure; Environmental Pollutants; Exposure to; Fishes; Food Contamination; Funding; Generations; Gnotobiotic; Goals; Grant; Human; Human Resources; Individual; Infrastructure; Interdisciplinary Study; Lead; Longevity; Manuscripts; Measures; Mediating; Mentors; Mentorship; Modality; Modeling; Molecular; National Institute of Environmental Health Sciences; Neurodevelopmental Impairment; Oral cavity; Oregon; Outcome; Parents; Poly-fluoroalkyl substances; Publishing Peer Reviews; Research; Research Project Grants; Research Training; Role; Science; Scientist; Series; Stretching; Systems Biology; Techniques; Technology; Toxicology; Training; Training Programs; Training Support; Underrepresented Students; Universities; Vertebrates; Veterans; Work; Writing; Zebrafish; analytical tool; base; behavioral impairment; biocomputing; bioinformatics resource; biomedical scientist; black women; career; career networking; contaminated water; doctoral student; early life exposure; environmental chemical; experience; experimental study; follow-up; graduate student; gut microbiome; improved; in vivo; interest; meetings; member; microbiome; microbiota; multidimensional data; neurodevelopment; neurodevelopmental effect; neurotoxicity; peer; pollutant; programs; skills; success; symposium; technology/technique; theories; tool; toxicant

PROJECT SUMMARY/ABSTRACT This supplement grant application aims to support the professional development of a Black, female, veteran graduate student who is pursuing a career as a toxicology research scientist. To accomplish this goal, the candidate’s co-mentors – Dr. Thomas Sharpton and Dr. Robyn Tanguay – have worked with the candidate to develop a research, training, and mentorship plan that will ensure the candidate’s professional success. This plan includes an extensive interdisciplinary training program that incorporates both didactic and experiential learning modalities, as well as a personalized, intimate, and supportive mentorship program. Additionally, the plan includes an interdisciplinary and cutting-edge research project that will help the candidate catapult their career forward. In particular, the candidate will conduct research that extends the R01 parent award into complimentary, but new areas. Briefly, the parent award focuses on defining how early life exposure to pollutants impacts assembly of the gut microbiome to impair behavior development, and focuses specifically on benzo[a]pyrene as a model pollutant. As part of this supplement grant, the candidate will apply the tools and technologies developed under the parent award to investigate a specific class of environmental chemicals of concern, namely a diverse array of per- and polyfluoroalkyl substances (PFAS) that have been shown to impair behavior development in vertebrates. In so doing, the candidate will resolve how embryonic exposure to various PFAS impacts gut microbiome assembly and whether these alterations to the gut microbiome mediate how PFAS impair behavior. As a result, the candidate will obtain interdisciplinary experience in an emerging area of toxicology, especially with respect to bioinformatic and data science approaches, and will clarify potential mechanisms through which PFAS elicit their effect on vertebrate behavior. In total, the proposed plan will equip a passionate junior scientist from an underrepresented community to thrive in the competitive research landscape.

项目概要/摘要 该补助金申请旨在支持黑人、女性、退伍军人的专业发展 正在从事毒理学研究科学家职业的研究生。为了实现这一目标， 候选人的共同导师 - Thomas Sharpton 博士和 Robyn Tanguay 博士 - 与候选人合作， 制定研究、培训和指导计划，以确保候选人的职业成功。这 计划包括广泛的跨学科培训计划，其中包括教学和体验式培训 学习方式，以及个性化、亲密和支持性的指导计划。此外， 计划包括一个跨学科和前沿的研究项目，该项目将帮助候选人提升他们的能力 职业生涯向前发展。特别是，候选人将进行研究，将 R01 家长奖扩展到 免费的，但新的领域。简而言之，家长奖的重点是定义早期生活中如何接触 污染物会影响肠道微生物组的组装，从而损害行为发展，并特别关注 苯并[a]芘作为模型污染物。作为补助金的一部分，候选人将应用这些工具和 根据母奖开发的技术，用于研究特定类别的环境化学品 令人担忧的问题，即多种全氟烷基物质和多氟烷基物质 (PFAS) 已被证明会损害 脊椎动物的行为发展。在此过程中，候选人将解决胚胎如何暴露于 各种 PFAS 会影响肠道微生物组的组装，以及这些对肠道微生物组的改变是否会介导 PFAS 如何损害行为。因此，候选人将在新兴的领域获得跨学科经验 毒理学领域，特别是在生物信息学和数据科学方法方面，并将澄清 PFAS 对脊椎动物行为产生影响的潜在机制。总体而言，拟议计划 将使来自代表性不足的社区的充满热情的初级科学家能够在竞争激烈的环境中茁壮成长 研究景观。