Impacts of Benzo[a]pyrene on Microbiome Development across Lifespan and Generations and the Behavioral Consequences

苯并[a]芘对整个生命周期和几代人微生物组发育的影响以及行为后果

• 批准号: 10544462
• 负责人: Thomas Jefferson Sharpton
• 金额: $ 6.23万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10544462
• 关键词: Active Learning; Adolescent; Affect; Applications Grants; Area; Attention; Award; Behavior; Behavioral; Benzo(a)pyrene; Bioinformatics; Biological; Biological Models; Chemicals; Communities; Data Science; Development; Development Plans; Educational workshop; Embryo; Enrollment; Ensure; Environmental Pollutants; Exposure to; Fishes; Food Contamination; Funding; Generations; Gnotobiotic; Goals; Grant; Human; Human Resources; Individual; Infrastructure; Interdisciplinary Study; Lead; Longevity; Manuscripts; Measures; Mediating; Mentors; Mentorship; Modality; Modeling; Molecular; National Institute of Environmental Health Sciences; Neurodevelopmental Impairment; Oral cavity; Oregon; Outcome; Parents; Poly-fluoroalkyl substances; Publishing Peer Reviews; Research; Research Project Grants; Research Training; Role; Science; Scientist; Series; Stretching; Systems Biology; Techniques; Technology; Toxicology; Training; Training Programs; Training Support; Underrepresented Students; Universities; Vertebrates; Veterans; Work; Writing; Zebrafish; analytical tool; base; behavioral impairment; biocomputing; bioinformatics resource; biomedical scientist; black women; career; career networking; contaminated water; doctoral student; early life exposure; environmental chemical; experience; experimental study; follow-up; graduate student; gut microbiome; improved; in vivo; interest; meetings; member; microbiome; microbiota; multidimensional data; neurodevelopment; neurodevelopmental effect; neurotoxicity; peer; pollutant; programs; skills; success; symposium; technology/technique; theories; tool; toxicant

PROJECT SUMMARY/ABSTRACT This supplement grant application aims to support the professional development of a Black, female, veteran graduate student who is pursuing a career as a toxicology research scientist. To accomplish this goal, the candidate’s co-mentors – Dr. Thomas Sharpton and Dr. Robyn Tanguay – have worked with the candidate to develop a research, training, and mentorship plan that will ensure the candidate’s professional success. This plan includes an extensive interdisciplinary training program that incorporates both didactic and experiential learning modalities, as well as a personalized, intimate, and supportive mentorship program. Additionally, the plan includes an interdisciplinary and cutting-edge research project that will help the candidate catapult their career forward. In particular, the candidate will conduct research that extends the R01 parent award into complimentary, but new areas. Briefly, the parent award focuses on defining how early life exposure to pollutants impacts assembly of the gut microbiome to impair behavior development, and focuses specifically on benzo[a]pyrene as a model pollutant. As part of this supplement grant, the candidate will apply the tools and technologies developed under the parent award to investigate a specific class of environmental chemicals of concern, namely a diverse array of per- and polyfluoroalkyl substances (PFAS) that have been shown to impair behavior development in vertebrates. In so doing, the candidate will resolve how embryonic exposure to various PFAS impacts gut microbiome assembly and whether these alterations to the gut microbiome mediate how PFAS impair behavior. As a result, the candidate will obtain interdisciplinary experience in an emerging area of toxicology, especially with respect to bioinformatic and data science approaches, and will clarify potential mechanisms through which PFAS elicit their effect on vertebrate behavior. In total, the proposed plan will equip a passionate junior scientist from an underrepresented community to thrive in the competitive research landscape.

项目摘要/摘要 这项补助申请旨在支持黑人、女性、退伍军人的职业发展 正在从事毒物学研究科学家职业的研究生。为了实现这一目标， 候选人的共同导师-托马斯·夏普顿博士和罗宾·坦盖博士-与候选人合作 制定研究、培训和指导计划，确保应聘者在职业上取得成功。这 计划包括一项广泛的跨学科培训计划，包括授课和体验两种形式 学习方式，以及个性化、贴心和支持性的指导计划。此外， 计划包括一个跨学科和尖端的研究项目，将帮助候选人迅速发展他们的 职业生涯向前。特别是，候选人将进行研究，将R01家长奖扩展到 免费的，但有新的领域。简而言之，家长奖的重点是定义早期生活中接触到的 污染物会影响肠道微生物群的组装，从而损害行为发育，并特别关注 苯并[a]芘作为模型污染物。作为这项补充补助金的一部分，候选人将应用这些工具和 根据家长奖开发的技术，用于调查特定类别的环境化学品 关注，即各种全氟烷基和多氟烷基物质(PFA)已被证明损害 脊椎动物的行为发育。通过这样做，候选人将解决胚胎如何暴露于 不同的PFAS影响肠道微生物组的组装，以及这些对肠道微生物组的改变是否在 PFAS是如何损害行为的。因此，应聘者将获得新兴学科领域的跨学科经验 毒理学领域，特别是在生物信息学和数据科学方法方面，并将澄清 全氟辛烷磺酸对脊椎动物行为产生影响的潜在机制。总体而言，拟议的计划 将使一位来自代表不足的社区的充满激情的初级科学家在竞争中茁壮成长 研究领域。