Facility Core E: Cell & Tissue Analysis

A.总体目标和具体目标 细胞和组织分析设施的核心包括流式细胞术和共聚焦显微镜服务。这个 流式细胞仪服务为中心调查人员提供了量化表面和/或细胞质的能力 蛋白质在多种细胞类型中的表达，分析凋亡细胞的比例，评估细胞周期 参数，并根据这些和其他标准将细胞分类为纯化的子集。共焦显微镜 通过定位细胞、细胞器或其他亚细胞，补充了流式细胞术的定量优势 在组织切片中使用高分辨率表达特定表面或细胞质蛋白质的成分 数字成像。 细胞和组织分析设施核心的具体目标是： 1.利用流式细胞术对单细胞悬液进行多参数分析和分离 通过细胞分选提纯亚群 2.利用共聚焦显微镜对蛋白质和亚细胞结构进行精确定位。 3.就规划、执行和评估涉及的实验提供培训和咨询 流式细胞术和共聚焦显微镜

A. Overall Objectives And Specific Aims The Cell and Tissue Analysis Facility Core includes flow cytometry and confocal microscopy services. The flow cytometry service provides Center investigators with the ability to quantify surface and/or cytoplasmic protein expression in a variety of cell types, analyze the fraction of apoptotic cells, evaluate cell cycle parameters and sort cells into purified subsets based on these and other criteria. Confocal microscopy complements the quantitative advantages of flow cytometry by localizing cells, organelles or other subcellular components that express particular surface or cytoplasmic proteins in tissue sections using high-resolution digital imaging. The Specific Aims of the Cell and Tissue Analysis Facility Core are: 1. To utilize flow cytometry for multiparameter analysis of single cell suspensions and for isolation of purified subsets by cell sorting 2. To utilize confocal microscopy for precise localization of proteins and subcellular structures. 3. To provide training and consultation on planning, executing and evaluating experiments involving flow cytometry and confocal microscopy