PROTEOMICS CORE

蛋白质组学核心

基本信息

批准号：
7907884
负责人：
MICHAEL ELDON GREENBERG
金额：
$ 21.56万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7907884
关键词：
Accounting Acetylation Affinity Chromatography Algorithms Amines Amino Acid Sequence Area Arts Attention Avidin Back Biopolymers Biotin Blood capillaries Buffers Cell Fraction Cell physiology Cells Charge Child Code Computer Simulation Computer software Computers Consultations Core Facility Coupled Cyclotrons Cysteine DNA Sequence Data Data Analyses Data Files Databases Detection Deuterium Development Developmental Disabilities Digestion Dimensions Disease Electrophoresis Electrospray Ionization Ensure Equipment Event Exclusion Explosion Fee-for-Service Plans Flavoring Floor Fourier transform ion cyclotron resonance Frequencies Future Gel Goals Healthcare Heating High Pressure Liquid Chromatography Housing Human Resources Hybrids Hydrogen Image Iodoacetamide Ion-Exchange Chromatography Procedure Ions Isotopes Label Lead Liquid Chromatography Manufacturer Name Mass Spectrum Analysis Measures Mental Retardation and Developmental Disabilities Research Centers Methods Methylation Modification Molecular Molecular Weight Molecular and Cellular Biology Nervous system structure Neurobiology Neurologic Neurons Neurosciences Neurosciences Research Ocular orbit Organism Pediatric Hospitals Peptide Sequence Determination Peptides Phase Phosphorylation Plasmids Population Post-Translational Protein Processing Postdoctoral Fellow Preparation Procedures Process Protein Isoforms Proteins Proteomics Protocols documentation Quality Control Reagent Recombinant Proteins Rehydrations Relative (related person)Research Research Personnel Resolution Sampling Scanning Science Scientist Services Side Signal Transduction Silver Staining Solutions Source Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spottings Stable Isotope Labeling Staging Staining method Stains Sulfhydryl Compounds System Techniques Technology Temperature Time Tissues Training Two-Dimensional Gel Electrophoresis analytical tool base capillary cost dalton data acquisition deamidation density design glycosylation graduate student improved instrument instrumentation interest magnetic field mass spectrometer member molecular mass nano-electrospray novel numb protein professor programs protein complex protein expression research study tandem mass spectrometry theories tool ultra high resolution voltage

项目摘要

2.a.2. Overall Objective The Proteomics Core aims to provide MRDDRC investigators with access to state of the art technologies in mass spectrometry and microscale capillary high performance liquid chromatography (proteomics) required for advanced studies in the cell and molecular biology of neurological developmental disabilities. Dr Greenberg (the Director of this MRDDRC) will be the Director of the Core. Dr. Jebanathirajah, a new Assistant Professor in the Neurobiology Program at Children's Hospital, and an expert in the field of mass spectrometry, will be the Manager responsible for supervising all Core activities. Major goals of the Core will be to use the techniques of mass spectrometry to assist MRDDRC investigators in the characterization of protein complexes that are relevant to neuronal function, to characterize changes in protein composition that occur in the nervous system during development and under conditions that lead to developmental disabilities, and to identify and characterize the function of post-translational modification of neuronal proteins. The Proteomics Core is actively involved in research in the area of mass spectrometry and any newly developed tools and methods will be made available to the MRDDRC investigators. The Core will also provide and develop proteomics related statistical and data handling tools.

2.A.2。总体目标蛋白质组学核心旨在为MRDDRC调查人员提供获得最先进技术的技术质谱和微观毛细管高性能液相色谱（蛋白质组学）神经发育障碍的细胞和分子生物学的高级研究。格林伯格博士（MRDDRC的主任）将担任核心主任。新助理教授Jebanathirajah博士在儿童医院的神经生物学计划中，以及质谱领域的专家将是负责监督所有核心活动的经理。核心的主要目标是使用这些技术质谱法以协助MRDDRC研究者的蛋白质复合物的表征与神经元功能相关，以表征神经系统中发生的蛋白质成分的变化在发展期间和导致发育障碍的条件下，并确定和表征神经元蛋白的翻译后修饰的功能。蛋白质组学核心是积极参与质谱领域以及任何新开发的工具和方法的研究可供MRDDRC调查人员使用。核心还将提供和开发与蛋白质组学相关的统计和数据处理工具。