Molecular Biology Shared Resource

构建和操作质粒和克隆的DMA序列是 所有参与癌症分子分析的实验室。有些操作更具挑战性 以及一些对普通程序不起作用的质粒体。分子生物学共享资源是 发起协助癌症中心研究人员进行质粒化操作和克隆项目，以证明 对个别实验室有问题或难以被实验室技术人员操纵， 学生和博士后。此外，分子生物学共享资源提供了性价比高的 为梅西癌症中心(MCC)的研究人员提供各种基本服务的替代方案。这些 基本服务包括无内毒素的质粒纯化、插入片段纯化、质粒管理和 维护质粒载体和克隆的清单。其他项目都是根据具体情况设计的 客户实验室的需求，包括定制亚克隆、文库筛选、生成和优化 蛋白质表达载体、基因打靶载体的设计、构建和分析以及蛋白质 在细菌、昆虫和哺乳动物细胞中生产。MBSR与转基因和 基因敲除核心设施，促进癌症中心成员通过载体接触这些动物 设计和施工。核心的很大一部分活动涉及到对具有 事实证明，对于提出请求的实验室来说，这很困难，因此每项工作往往都有独特的特点，通常 包括故障排除和程序的重新设计。因此，设施人员的专业知识已经证明 成为核心所提供的最有价值的服务之一。MSBR人员提供培训研讨会和 预览新技术的发展，并经常培训实验室人员进行常规(和 不是那么常规)分子程序。核心主任提供分子所有阶段的正式课程 可以记入信用或被审计的技术。 分子生物学共享资源的新方向正在不断地寻求，在 咨询癌症中心调查人员和MBSR监督委员会。最近雇用的新员工 化学生物学系进入化学和生物化学系(分子学院成员 癌症治疗计划)促进了支持蛋白质生产的结构 学习。共享资源在过去两年中日益活跃，协助调查人员 根据他们的蛋白质生产需求，生成表达载体，优化蛋白质表达 用于扩大生产的系统。

The construction and manipulation of plasmids and of cloned DMA sequences is a central activity of every laboratory involved in the molecular analysis of cancer. Some manipulations are more challenging and some plasmids refractory to common procedures. The Molecular Biology Shared Resource was initiated to assist Cancer Center investigators with plasmid manipulations and cloning projects that prove problematic to individual laboratories or that prove refractory to manipulation by laboratory technicians, students, and post-docs. In addition, the Molecular Biology Shared Resource provides a cost-effective alternative to a variety of basic services to the investigators of the Massey Cancer Center (MCC). These basic services include endotoxin-free plasmid purification, insert purification, plasmid management and maintaining an inventory of plasmid vectors and clones. Other projects are designed upon the specific needs of client laboratories including custom subcloning, library screening, generation and optimization of protein expression vectors, design, construction, and analysis of gene targeting vectors, and protein production in bacterial, insect and mammalian cells. The MBSR works in synch with the Transgenic and Knockout Core Facility and facilitates access of Cancer Center members to such animals through vector design and construction. A large part of the activity of the Core involves plasmid manipulations that have proven difficult for the requesting laboratory, so each job tends to have unique characteristics and usually involves trouble-shooting and redesign of procedures. Thus, the expertise of Facility personnel has proven to be one of the most valuable services offered by the Core. MSBR personnel offer training seminars and previews of newer technologies as they are developed, and often train laboratory personnel in routine (and not so routine) molecular procedures. The Core Director offers a formal course in all phases of molecular technique that can be taken for credit or audited. New directions for the Molecular Biology Shared Resource are continuously being sought, in consultation with Cancer Center investigators and the MBSR oversight committee. The recent hire of new faculty in Chemical Biology into the Departments of Chemistry and Biochemistry (members of the Molecular Cancer Therapeutics program) has promoted a move towards the support of protein production for structural studies. The Shared Resource has been increasingly active during the past 2 years assisting investigators with their protein production needs, generating expression vectors and optimizing protein expression systems for scaled-up production.