Gene Targeting Core Facility

基因靶向核心设施（GTCF）是一个完全建立的核心资源，为以下方面提供专家服务： 胚胎干细胞（ES）基因靶向用于小鼠模型的开发。主服务 这个核心所提供的是采用研究者的基因靶向载体并产生ES细胞系， 可以发展成通过其种系传递相关基因组修饰的嵌合小鼠。 利用这个核心的研究人员已经成功地产生了各种基因敲除和小鼠 疾病的模型。GTCF是UAB转基因小鼠设施的一个组成部分， 共同提供专家服务，不仅通过GTCF基因靶向，而且囊胚 显微注射靶向的ES细胞克隆或DNA构建体用于产生转基因小鼠模型， 以及与小鼠研究相关的大量服务（例如胚胎的冷冻保存，再衍生， 等）。转基因动物设施与UAB的其他核心资源有战略联系，包括 CCC小动物影像核心，临床营养研究中心小动物表型核心， 用于表型评估的动物病理学研究实验室和专门的小鼠育种核心 （Genetically Engineered Mouse and Marker-Assisted Genetics Core）总的来说，我们拥有一流的资源， 为中心研究人员制作和表征小鼠模型。

The Gene Targeting Core Facility (GTCF) is a fully established core resource to provide expert services for gene targeting of embryonic stem (ES) cells for the development of mouse models. The primary service orovided by this core is that of taking an investigator's gene targeting vector and producing ES cell lines that can be developed into chimeric mice that transmit the pertinent genomic modification(s) through their germline. Investigators utilizing this core have successfully produced a variety of gene knockouts and mouse models of disease. The GTCF is an integral component of the UAB Transgenic Mouse Facility that collectively provides expert services for not only gene targeting via the GTCF, but also blastocyst micro-injection of targeted ES cell clones or DNA constructs for the production of transgenic mouse models, as well as a host of services related to research with mice (e.g. cryopreservation of embryos, rederivation, etc.). The Transgenic Animal Facility is strategically linked with other core resources at UAB including the CCC Small Animal Imaging Core, Clinical Nutrition Research Center Small Animal Phenotyping Core, Research Animal Pathology Laboratory for phenotype assessment, and a specialized mouse-breeding core (Genetically Engineered Mouse and Marker-Assisted Genetics Core). Overall, we have premier resources to make and characterize mouse models for the Center investigators.