INTEGRATED MICROSCOPY

集成显微镜

• 批准号: 7714285
• 负责人: BENJAMIN S GLICK
• 金额: $ 7.15万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-22 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714285
• 关键词: Area; Arts; Biochemical; Cancer Center Support Grant; Cells; Collaborations; Color; Communities; Computer software; Computers; Condition; Data; Decision Making; Dimensions; Equipment; Fluorescence; Fluorescence Microscopy; Fostering; Funding; Generations; Hour; Human Resources; Image; Image Analysis; Imaging technology; Individual; Knowledge; Laboratories; Learning; Life; Methods; Microscope; Microscopy; Mission; Operative Surgical Procedures; Optics; Peer Review; Physiological; Pilot Projects; Population; Preparation; Publications; Qualifying; Range; Reliance; Reporting; Research; Research Personnel; Resolution; Resources; Running; Services; Speed; Staining method; Stains; Sum; System; Techniques; Technology; Testing; Time; Training; Universities; University of Chicago Cancer Research Center; Update; Work; anticancer research; base; cost; design; desire; improved; instrument; instrumentation; member; millimeter; nanometer; programs; research study; tool; wasting

The Integrated Microscopy Facility (IMF) functions as a supervised, user-based Core providing state-of-theart microscopy imaging capabilities to UCCRC members and other University investigators. The primary mission of the Facility is to provide a valuable, unique service to the University community by furnishing highquality microscopy instrumentation, image analysis tools, and expert assistance. In addition, maintaining equipment in optimal condition and training users require skilled technical personnel, something that individual laboratories typically cannot afford. Because imaging technology and computational capacity are evolving so rapidly, it is extremely difficult for individual researchers to maintain or afford cutting-edge capabilities. Integrated Microscopy offers one of the most flexible and informative tools available to researchers, offering scales of detail ranging from tens of millimeters to less than 10 nanometers with appropriate techniques, Available techniques provided by the IMF range from classic color histological stain imaging, to contrast generation in unstained cells, to fluorescence technologies. Fluorescence methods continue to offer the greatest range of applications ranging from localization of multiple targets to readout of biochemical or physiologic parameters in either fixed or living preparations. Accordingly, the greatest resources of the IMF are allocated to fluorescence microscopy. By merging the microscopy facilities created by several campus entities, the combined pool of resources is more efficiently managed and maintained, while also increasing access, capabilities, and capacity for UCCRC member research. Overall use of the IMF has increased close to three times the activity reported in the 2001 CCSG submission. Over 63 peer-reviewed investigators across six Scientific Programs routinely use the IMF, totaling 49% of Facility usage. Services provided by the IMF consist of the following: ¿ Provide state-of-the-art microscopy imaging capabilities including brightfield (color, DIG, etc), fluorescence (multi-dimension; TIRFM; bleaching, etc), high-resolution, -speed, -sensitivity, confocal, and physiologic techniques. ¿ Provide training on all instruments and expert advice to investigators regarding experiment design and execution. Provide oversight to assure proper equipment use and minimize breakage. ¿ Provide computers including networked data servers and software for image analysis and publication. ¿ Serve as an educational resource for University investigators in the use of current microscopy techniques and foster interactions among users.

综合显微镜设施（IMF）作为一个受监督的、以用户为基础的核心， UCCRC成员和其他大学研究人员的显微镜成像能力。主 该设施的使命是通过提供高质量的服务，为大学社区提供有价值的，独特的服务。 显微镜仪器、图像分析工具和专家协助。此外，维护 设备处于最佳状态和培训用户需要熟练的技术人员， 个别实验室通常无法负担。因为成像技术和计算能力 发展如此之快，对于单个研究人员来说，保持或负担得起尖端技术是非常困难的。 能力的 集成显微镜为研究人员提供了最灵活和信息量最大的工具之一， 利用适当的技术可以实现从几十毫米到小于10纳米的细节尺度， IMF提供的可用技术范围从经典的彩色组织学染色成像到对比成像， 在未染色的细胞中产生，荧光技术。荧光方法继续提供 最大的应用范围，从多个目标的定位到生物化学或 生理参数在固定或活的准备。因此，国际货币基金组织的最大资源 被分配到荧光显微镜。通过合并几个校园创建的显微镜设施， 通过对各实体进行合并，合并后的资源库得到更有效的管理和维护，同时也增加了 UCCRC成员研究的访问、能力和容量。对货币基金组织的总体使用增加了近 是2001年CCSG报告中报告的活动的三倍。超过63名同行评审研究者 在六个科学计划中，基金组织经常使用基金，占基金使用量的49%。 货币基金组织提供的服务包括： 提供最先进的显微成像功能，包括明场（彩色、DIG等）， 荧光（多维; TIRFM;漂白等），高分辨率，高速，高灵敏度，共焦， 和生理技术。 提供所有仪器的培训，并就实验设计向研究人员提供专家建议 和处决提供监督，以确保正确使用设备并尽量减少破损。 提供计算机，包括联网数据服务器和图像分析和发布软件。 作为大学研究人员使用当前显微镜的教育资源 技术和促进用户之间的互动。