Analytical Core

分析核心将为调查人员提供各种专业的分析服务 协助进行分子、细胞和活体研究的计划项目。这个 分析核心将是索尔克研究所各单位之间在以下指导下的合作努力 罗纳德·埃文斯博士，还有一位在加州大学圣地亚哥分校约瑟夫博士手下 维茨塔姆。分析核心的总体目标是利用专业资源和 为调查员提供协助研究任务的选定核心服务的专门知识 每个项目的。这一核心的目标是： 具体目标1：提供高通量定量聚合酶链式反应(HT-QPCR)分析 靶基因，如核受体或影响炎症的靶基因和 动脉硬化。该平台将允许快速探索各种影响机制 巨噬细胞和B-1细胞核激素受体表达的扰动 其他细胞或组织。 具体目标2：提供高通量和高灵敏度的细胞因子和 通过使用BioPlex在小样本体积中的趋化因子，如血浆或培养液 悬浮阵列蛋白质组图谱。 具体目标3：提供免疫学服务，包括产生抗原特异性 抗血清和单抗、试剂的制备和多种抗体的性能 免疫学检测，例如，正在研究的小鼠模型的血浆抗体水平。 具体目标4：提供脂类和脂蛋白的定量和定性分析，以制备 需要分离的脂蛋白组分，如低密度脂蛋白，以及提供标准化的修饰 脂蛋白，如氧化低密度脂蛋白和低密度脂蛋白，供所有研究人员使用。

The Analytical Core will provide a variety of specialized analytical services for the investigators of the Program Project to assist in the conduct of the molecular, cellular and in vivo studies. The Analytical Core will be a collaborative effort between units at the Salk Institute under the direction of Dr. Ronald Evans, and one located at the University of California, San Diego under Dr. Joseph Witztum. The overall goal of the Analytical Core is to take advantage of specialized resources and expertise to provide investigators with selected core services that will assist in the research mission of each Project. The Aims of this Core are: Specific Aim 1: To provide high throughput quantitative PCR analysis (HT-QPCR) of selected target genes, such as nuclear receptors or target genes that impact inflammation and atherosclerosis. This platform will allow rapid exploratory mechanism of impact of various perturbations on nuclear hormone receptor expression in macrophages and B-1 cells, as well as in other cells or tissue. Specific Aim 2: To provide high throughput and highly sensitive analysis of cytokines and chemokines in small sample volumes, such as plasma or culture fluids, through use of Bioplex suspension array proteomic profiling. Specific Aim 3: To provide immunological services including the generation of antigen-specific antisera and monoclonal antibodies, preparation of reagents and performance of a variety of immunological assays, as for example, antibody levels in plasma of murine models under study. Specific Aim 4: To provide quantitative and qualitative analysis of lipids and lipoproteins, to prepare isolated lipoprotein fractions needed, such as LDL, as well as provision of standardized modified lipoproteins, such as oxidized LDL and mmLDL for use by all investigators.