Genomics Facility

基因组学设施（GF）于2001年9月开始向用户提供高通量微阵列服务。 其目标是提供高质量，价格合理的基因分析技术平台，以满足 研究人员的研究需求。GF为调查人员提供了接触人员的机会， 在各种基因组技术方面经验丰富， 自己它避免了为实施《公约》所需的人员、设施和设备的昂贵重复。 这些不断发展的方法。绿色基金提供全方位的服务， 实验设计，基因表达分析，数据解释，通过紧密结合， 生物信息学系。GF位于700平方米。英尺实验室空间和计算机办公室 位于最先进的基础科学研究大楼-北校区。GF提供DNA， 针对多种微阵列应用的RNA分析服务，包括基因表达、单核苷酸 多态性（SNP）、比较基因组杂交（CGH）、染色质免疫沉淀（ChIP）、 芯片）和microRNA分析。主要设备包括Affyssin基因芯片系统，Axon仪器 GenePix 4000 B扫描仪，NimbleGen杂交系统，Affyphon高通量阵列工作站， Genomics Solutions Flexys阵列仪、Agilent BioAnalyzer和高通量PCR仪。的 实验室有3名教师（包括主任和副主任）和3名支持人员。提供监督 由8名高级领导人组成的多学科监督委员会。网上访问GF是通过 实验室信息管理系统（LIMS）。LIMS使用户能够跟踪其项目的状态 和在线服务请求。自2005年9月启动以来，81个项目和300项服务请求 已在LIMS上提交，为63名主要研究者和142名用户提供服务。拟议供资用于 来自癌症中心支持补助金（30%），用户费用（55%）和机构资源（15%）。 在过去的5年中，全球基金从为102名调查员提供服务的用户费中获得了50%-65%的财政支持 21个CCSG项目中的17个。74%的用户有同行评审的资金，占85%的 利用率该设施的用户已经发表了使用基因组学的肿瘤生物学的重要论文 方法、基因组学技术的发展、基因组学分析的生物信息学和生物统计学。 未来的计划包括根据要求开发、生产和实施新的微阵列平台 研究人员和扩展现有平台上的服务，包括 NimbleGen平台和Affytron平台上的microRNA分析

The Genomics Facility (GF) began offering high-throughput microarray services to users in September 2001. Its goal is to provide high quality, reasonably-priced, gene-profiling technology platforms to meet the research needs of NCI-funded investigators. The GF provides investigators with access to personnel who are highly-experienced in a variety of genomic technologies as well as access to the methodologies themselves. It avoids expensive duplication of personnel, facilities and equipment required for application of these constantly-evolving methodologies. The GF provides a full range of services, from consultation on experimental design to gene expression analyses to data interpretation via close integration with the Department of Bioinformatics. The GF is housed in 700 sq. ft. of laboratory space and computer office space in the state-of-the-art Basic Science Research Building - North Campus. The GF provides DNA and RNA analysis services for several microarray applications, including gene expression, single nucleotide polymorphism (SNP), Comparative Genomic Hybridization (CGH), Chromatin Immunoprecipitation (ChIP on Chip) and microRNA profiling. Major equipment includes Affymetrix GeneChip system, Axon Instruments GenePix4000B scanner, NimbleGen Hybridization System, Affymetrix High-throughput Array Station, Genomics Solutions Flexys arrayer, Agilent BioAnalyzer, and high-throughput PCR machines. The laboratory has 3 faculty (including Director and Co-Director) and 3 support personnel. Oversight is provided by a multidisciplinary Oversight Committee of 8 senior leaders. Online access to the GF is provided via the Laboratory Information Management System (LIMS). LIMS enables users to track the status of their projects and service requests on-line. Since its launch in September 2005, 81 projects and 300 requests for services have been submitted on LIMS serving 63 principal investigators and 142 total users. Funding is proposed to come from the Cancer Center Support Grant (30%), users' fees (55%), and institutional resources (15%). During its last 5 years, the GF received 50%-65% financial support from user fees serving 102 investigators from 17 of the 21 CCSG programs. 74% of the users had peer-reviewed funding and accounted for 85% of utilization. Users of the facility have published important papers in tumor biology using genomics approaches, development of genomics technology, bioinformatics and biostatistics of genomics analysis. Future plans include development, production and implementation of new microarray platforms as requested by the investigators and expansion of services on the existing platforms including methylation assay on the NimbleGen platform and microRNA profiling on the Affymetrix platform