Genomics Facility

基因组学设施(GF)于2001年9月开始向用户提供高通量微阵列服务。 其目标是提供高质量、合理价格的基因图谱技术平台，以满足 NCI资助的研究人员的研究需求。GF为调查人员提供了接触以下人员的途径 在各种基因组技术以及方法学方面经验丰富 他们自己。它避免了昂贵的人员、设施和设备的重复应用 这些不断演变的方法。广交会提供全方位的服务，从咨询到 从基因表达分析到数据解释的实验设计，通过与 生物信息学系。广域酒店占地700平方米。英国《金融时报》实验室空间和计算机办公室 最先进的基础科学研究大楼-北校区的空间。GF提供DNA和 为多种微阵列应用提供RNA分析服务，包括基因表达、单核苷酸 多态(SNP)、比较基因组杂交(CGH)、染色质免疫沉淀(ChIP on 芯片)和microRNA简档。主要设备包括Affymetrix基因芯片系统、Axon仪器 GenePix4000B扫描仪，NimbleGen杂交系统，Affymetrix高通量阵列站， 基因组学解决方案Flexys Arrayer、安捷伦生物分析仪和高通量PCR机。这个 实验室有3名教职员工(包括主任和联席主任)和3名辅助人员。提供了监督 由8名高级领导人组成的多学科监督委员会。可通过以下方式在线访问GF 实验室信息管理系统(LIMS)。LIMS使用户能够跟踪其项目的状态 和在线服务请求。自2005年9月启动以来，81个项目和300项服务请求 已在LIMS上提交，为63名主要调查人员和142名用户提供服务。建议提供资金以 来自癌症中心支持拨款(30%)、用户费用(55%)和机构资源(15%)。 在过去的5年里，广电总局从102名调查人员的使用费中获得了50%-65%的财务支持 来自21个CCSG项目中的17个。74%的用户有同行评审的资金，占85% 利用率。该设备的使用者已经发表了利用基因组学进行肿瘤生物学研究的重要论文 基因组学分析的方法、基因组学技术的发展、生物信息学和生物统计学。 未来计划包括应要求开发、生产和实施新的微阵列平台 由调查人员提供，并在现有平台上扩展服务，包括在 NimbleGen平台及在Affymetrix平台上的microRNA剖析

The Genomics Facility (GF) began offering high-throughput microarray services to users in September 2001. Its goal is to provide high quality, reasonably-priced, gene-profiling technology platforms to meet the research needs of NCI-funded investigators. The GF provides investigators with access to personnel who are highly-experienced in a variety of genomic technologies as well as access to the methodologies themselves. It avoids expensive duplication of personnel, facilities and equipment required for application of these constantly-evolving methodologies. The GF provides a full range of services, from consultation on experimental design to gene expression analyses to data interpretation via close integration with the Department of Bioinformatics. The GF is housed in 700 sq. ft. of laboratory space and computer office space in the state-of-the-art Basic Science Research Building - North Campus. The GF provides DNA and RNA analysis services for several microarray applications, including gene expression, single nucleotide polymorphism (SNP), Comparative Genomic Hybridization (CGH), Chromatin Immunoprecipitation (ChIP on Chip) and microRNA profiling. Major equipment includes Affymetrix GeneChip system, Axon Instruments GenePix4000B scanner, NimbleGen Hybridization System, Affymetrix High-throughput Array Station, Genomics Solutions Flexys arrayer, Agilent BioAnalyzer, and high-throughput PCR machines. The laboratory has 3 faculty (including Director and Co-Director) and 3 support personnel. Oversight is provided by a multidisciplinary Oversight Committee of 8 senior leaders. Online access to the GF is provided via the Laboratory Information Management System (LIMS). LIMS enables users to track the status of their projects and service requests on-line. Since its launch in September 2005, 81 projects and 300 requests for services have been submitted on LIMS serving 63 principal investigators and 142 total users. Funding is proposed to come from the Cancer Center Support Grant (30%), users' fees (55%), and institutional resources (15%). During its last 5 years, the GF received 50%-65% financial support from user fees serving 102 investigators from 17 of the 21 CCSG programs. 74% of the users had peer-reviewed funding and accounted for 85% of utilization. Users of the facility have published important papers in tumor biology using genomics approaches, development of genomics technology, bioinformatics and biostatistics of genomics analysis. Future plans include development, production and implementation of new microarray platforms as requested by the investigators and expansion of services on the existing platforms including methylation assay on the NimbleGen platform and microRNA profiling on the Affymetrix platform