In-Vivo, Wireless Fast-Scan Cyclic Voltammetry System

描述（由申请人提供）：品尼高技术和堪萨斯大学的乔治威尔逊和迈克尔约翰逊教授开发并成功测试了一种原型设备，该设备可以在自由移动的大鼠中无线收集、分析和传输快速扫描循环伏安法（FSCV）数据。原型FSCV系统与微电极（直径<10 5 m）兼容。该系统可以测量细胞外多巴胺的生理相关水平。在第二阶段的工作中，一个完整的交钥匙，无线系统将开发，测试和验证多巴胺。还将测试检测其他单胺神经递质（如去甲肾上腺素和血清素）的能力。这项工作将包括电极的开发与集成连接器（5-50 5米直径），集成的刺激，结合微驱动器，优化的检测极限，扩展到多个电极，如果可能的话，并完成一个商业上可行的采集和分析软件包。分析包将包括与外部刺激、视频或其他相关事件的同步。该系统将由独立实验室进行内部验证和beta测试。该产品的开发是当前最先进技术的一个重大进步，它将使研究人员能够测量自发的亚秒级神经递质释放事件，同时在自由移动的动物中进行详细的行为研究。对无束缚动物的行为研究提供了正常行为与功能性神经化学之间联系的关键知识，无线快速循环伏安法系统将成为行为，记忆，学习，成瘾机制和睡眠研究人员的主要资产。公共卫生相关性：多巴胺信号异常严重影响的两种疾病是精神分裂症和帕金森病。精神分裂症影响了大约290万美国人。大约有150万美国人患有帕金森氏症。根据2000年的估计，精神分裂症和帕金森氏症每年的直接健康成本为700亿美元。

DESCRIPTION (provided by applicant): Pinnacle Technology and Professors George Wilson and Michael Johnson of the University of Kansas, have developed, and successfully tested, a prototype device that can collect, analyze and transmit fast-scan cyclic voltammetry (FSCV) data wirelessly in freely moving rats. The prototype FSCV system is compatible with microelectrodes (<10 5m diameter). The system can measure physiologically relevant levels of extracellular dopamine. During this Phase II effort, a complete turnkey, wireless system will be developed, tested, and validated for dopamine. The ability to detect other monoamine neurotransmitters such as norepinephrine, and serotonin will also be tested. This work will include the development of electrodes with integrated connectors (5-50 5m diameter), integrated stimulus, incorporation of a microdrive, optimization of the limit of detection, expansion to multiple electrodes if possible, and completion of a commercially viable acquisition and analysis software package. The analysis package will include synchronization with external stimuli, video or other related events. The system will be validated internally and beta tested by independent laboratories. Development of the proposed product is a highly significant advancement over the current state-of-the art. It will empower researchers to measure spontaneous, sub-second neurotransmitter release events while conducting detailed behavioral studies in freely moving animals. Behavioral studies in untethered animals provide critical knowledge of the link between normal behavior and functional neurochemistry, and a wireless fast cyclic voltammetry system will be a major asset for researchers in behavior, memory, learning, addiction mechanisms and sleep. PUBLIC HEALTH RELEVANCE: Two conditions heavily influenced by abnormal dopamine signaling are schizophrenia and Parkinson's disease. Schizophrenia affects an estimated 2.9 million Americans. Approximately 1.5 million Americans suffer from Parkinson's. Based on year 2000 estimates, the yearly direct health costs due to Schizophrenia and Parkinson's is 70 billion dollars.