A self-contained wireless modular separation based sensor system

描述（由申请人提供）：最近的进展表明，使用毛细管电泳（CE）进行有效的神经递质分析。在CE系统中，使用离子运动将分析物通过窄通道抽取，从而导致由高压电场产生的电渗流（electrosomious flow，简称E渗流）。文献中报道的大多数微芯片CE方法对每个通道使用相同的分离和检测方法，目标是单一类型分析物的高样品通量。最初由堪萨斯的Lunte实验室开发的成功的芯片实验室分析系统将被完善，以创建一个模块化的、独立的、交钥匙工具，用于从生物组织收集、分离分析和无线传输所收集的分析物。该设备的商业化将导致集成的高压电源，CE分析芯片，液体泵送系统和无线数据传输单元足够坚固，可用于远程现场设置，并易于为一般研究社区所用。拟议产品的开发是当前最先进技术的一个非常重要的进步，因为它将使研究人员能够对神经递质和一氧化氮代谢物进行更详细的定期测量，同时将结果与行为研究联系起来。这些观察结果提供了关于正常行为和功能神经化学之间联系的重要知识。这种设计的模块化组件将形成更复杂的分析系统的基础，用于多种分析物，如肽和氨基酸，只需改变芯片和分析仪器包，同时保持系统的其余部分不变。完成后，该提案将通过在任何实验室或现场环境中轻松实施强大的便携式微透析采样和分析工具，将Lunte集团先前开发的有用的芯片实验室技术推向商业市场。公共卫生相关性：在一个独立的便携式设备中连续采样和分析大脑神经递质水平或组织液，将为研究人员提供新的方法来观察与帕金森氏症或阿尔茨海默氏症等许多疾病相关的神经化学变化。根据阿尔茨海默氏症协会（一家医疗保健研究机构）最近的报告，痴呆症和神经退行性疾病可能会影响五分之一的婴儿潮一代，到2015年，美国医疗保健系统的成本可能高达2000亿美元。

DESCRIPTION (provided by applicant): Recent advances have shown effective neurotransmitter analysis using capillary electrophoresis (CE). In a CE system, analyte is drawn through a narrow channel using ion movement resulting in an electroosmotic flow (EOF) generated by a high voltage electric field. Most microchip CE methods reported in the literature use the same separation and detection method for every channel with the goal of high sample throughput of a single type of analyte. The successful lab- on-chip analysis system initially developed by the Lunte laboratory at the University of Kansas will be refined to create a modular, self-contained, turnkey tool for collection, separation analysis and wireless transmission of collected analyte from biological tissue. Commercialization of this device will result in an integrated high voltage power source, CE analysis chip, liquid pumping system and wireless data transmission unit robust enough for use in a remote field setting and easily accessible to the general research community. Development of the proposed product is a highly significant advancement over the current state-of-the art as it will empower researchers to make more detailed, regular measurements of neurotransmitter and nitric oxide metabolites while tying results to behavioral studies. Such observations provide critical knowledge of the link between normal behavior and functional neurochemistry. The modular components of this design will form the basis for a more sophisticated system of analysis for multiple classes of analytes such as peptides and amino acids simply by changing the chip and analysis instrument package while leaving the rest of the system unchanged. When complete, this proposal will move useful lab-on-chip technology previously developed by the Lunte group into the commercial marketplace by making a robust, portable microdialysis sampling and analysis tool easily implemented in any lab or field environment. PUBLIC HEALTH RELEVANCE: Continuous sampling and analysis of brain neurotransmitter levels or tissue fluid in a self- contained, portable device will give researchers new ways to observe neurochemical changes associated with many disorders such as Parkinson's or Alzheimer's. According to recent reports by the Alzheimer's Association an Agency for Healthcare research, dementia and neurodegenerative disorders are likely to affect 1 in 5 baby boomers and could cost the U.S. health care system as much as $200 billion by 2015.