CB: Tissue Core

Tjssue Bank Core的目标是帮助SPORE研究人员：1）识别肿瘤生物学特性， 分子遗传学相关的诊断，预后，反应，进展和生存的背景下， 与SPORE相关的调查; 2）维护可检索的临床和实验室数据数据库， 供孢子研究者使用; 3）为未来的研究提供库存标本资源。核心 实验室功能旨在提高单个项目检测生物 进入SPORE研究的患者之间的差异，并确定反应的分子相关性 相对于阻力，并具有足够的灵活性，以满足个体研究者的需求。上一 在资助期间，组织核心位于完善的马约诊所骨髓瘤核心设施， 接收、处理、存储、存档和分发临床注释的细胞（骨髓和PB 细胞）和分子（DNA和RNA）以及血清样本提供给参与此次SPORE的研究者。 来自SPORE的样本已与其他核心资源分开收集、测试和存档， 如ECOG或马约计划项目参考实验室;然而，这些资源 在这些核心资源之间共享，从而增加了可用于相关分析的样本池。 科学研究者们。由于我们不断增长的SPORE和密切合作的需求增加 并与马约诊所的核心合作，我们现在已经在 达纳·法伯癌症研究所为了使马约诊所和DFCI的努力天衣无缝， 为了兼容注释样本共享，我们使用了相同的骨标准操作程序 骨髓处理、骨髓瘤细胞纯化和冷冻保存。我们的生物统计和临床数据 这两个机构的管理被整合到核心3中，这允许共享数据和兼容性 在研究的样本选择上。这个核心提供了一个独立的，最新的，快速搜索 临床和实验室结果以及存档生物标本的数据库。数据表填写时间 样本的收集被输入到共同的临床、实验室和档案数据库中。跟进 根据需要获取并输入数据。然后，所有数据都存储在可由 统计中心。所有患者均测量了SPORE要求的关键生物学变量 investigators.保存未使用的标本，包括分选细胞、DNA、RNA和细胞离心涂片，以备将来使用 用于所有患者。后续使用超出本SPORE提案规定的库存样本将 仅在获得SPORE总监和SPORE主要研究者同意的情况下提供。该芯 由Fonseca博士和Munshi博士领导，他们在管理多机构核心实验室方面经验丰富， 合作设置。

The goals of the Tjssue Bank Core are to aid SPORE investigators: 1) to identify tumor biologic and molecular genetic correlates of diagnosis, prognosis, response, progression, and survival in the context of the SPORE related investigations ; 2) to maintain a searchable database of clinical and laboratory data for use by SPORE investigators; and 3) to provide a resource for banked specimens for future studies. Core Laboratory functions are designed to increase the power of the individual projects to detect biologic differences among patients entering the SPORE studies and identify molecular correlates of response versus resistance, and to be flexible enough to address individual investigator needs. In the previous funding period, the tissue core was located at the well established Mayo Clinic Myeloma Core facilities which received, processed, stored, archived and distributed clinically annotated cellular (bone marrow and PB cells) and molecular (DMA and RNA) and serum samples to investigators participating in this SPORE. Samples from the SPORE have been collected, tested, and archived separately from other Core resources, such as the ECOG or the Mayo Program Project reference laboratories; however, these resources have been shared between these cores resources, thereby increasing the pool of samples available for correlative science by the SPORE investigators. Due to the increased needs of our growing SPORE and in close cooperation and collaboration with the core at the Mayo clinic, we have now established a tissue core facility at the Dana Farber Cancer Institute. To make efforts at the Mayo Clinic and at the DFCI seamless and compatible for annotated sample sharing, we have utilized the same standard operating procedures for bone marrow processing, myeloma cell purification, and cryopreservation. Our biostatical and clinical data management at both the institutions are integrated in Core 3, which allows sharing of data and compatibility in sample selection for research studies. This Core provides an independent, current, and quickly searchable database of clinical and laboratory results and archived biospecimens. Data forms completed at the time of collection of the samples are entered into a common clinical, laboratory, and archival database. Follow up data is obtained and entered as required. All data are then stored in a common database accessible by the Statistics center. All patients have measurement of key biological variables requested by SPORE investigators. Unused specimen including sorted cells, DNA, RNA and cytospin slides are stored for future use on all patients. Subsequent use of banked samples beyond that specified in this SPORE proposal will be provided only with agreement from SPORE Director and the SPORE Principal Investigators. This Core is lead by Drs. Fonseca and Munshi, who are experienced in managing Core laboratories in a multi-institutional cooperative setting.