CB: Tissue Core

Tjssue Bank Core的目标是帮助孢子调查人员：1)识别肿瘤生物学和 与诊断、预后、反应、进展和生存相关的分子遗传学 与孢子有关的调查；2)维持一个可检索的临床和实验室数据数据库，以 供孢子调查人员使用；以及3)为未来研究提供保存标本的资源。堆芯 实验室功能的设计是为了增加单个项目检测生物的能力 进入孢子研究的患者之间的差异并确定反应的分子相关因素 对抗阻力，并具有足够的灵活性，以满足个别调查员的需求。在上一次 在供资期间，组织核心位于完善的梅奥诊所骨髓瘤核心设施， 接收、处理、存储、存档和分发临床注释的细胞(骨髓和外周血 细胞)和分子(DNA和RNA)和血清样本提供给参与这一孢子的研究人员。 来自孢子的样本已经与其他核心资源分开收集、测试和归档， 如经社理事会或梅奥方案项目参考实验室；然而，这些资源有 在这些岩心资源之间共享，从而增加了可供对比的样本库 孢子调查员的科学研究。由于我们日益增长的孢子需求和密切的合作 并与梅奥诊所的核心合作，我们现在已经在 达纳·法伯癌症研究所。在梅奥诊所和DFCI做出无缝和 兼容带注释的样本共享，我们对骨骼使用了相同的标准操作程序 骨髓加工、骨髓瘤细胞纯化、冷冻保存。我们的生物统计和临床数据 这两个机构的管理都集成在Core 3中，允许共享数据和兼容性 在研究研究的样本选择上。该核心提供了独立的、最新的、可快速搜索的 临床和实验室结果数据库以及存档的生物标本数据库。在以下日期填写的数据表格 样本的收集被输入到共同的临床、实验室和档案数据库中。跟进 根据需要获取并输入数据。然后，所有数据都存储在一个公共数据库中， 统计中心。所有患者都有孢子要求的关键生物学指标的测量 调查人员。未使用的样本，包括分类的细胞、DNA、RNA和细胞质玻片被储存起来，以备将来使用 在所有患者身上使用。以后使用超出本孢子提案中规定范围的库存样品将 只有在孢子主管和孢子首席调查员同意的情况下才能提供。这个核心是 由Fonseca博士和Munshi博士领导，他们在管理多机构的核心实验室方面经验丰富 合作环境。

The goals of the Tjssue Bank Core are to aid SPORE investigators: 1) to identify tumor biologic and molecular genetic correlates of diagnosis, prognosis, response, progression, and survival in the context of the SPORE related investigations ; 2) to maintain a searchable database of clinical and laboratory data for use by SPORE investigators; and 3) to provide a resource for banked specimens for future studies. Core Laboratory functions are designed to increase the power of the individual projects to detect biologic differences among patients entering the SPORE studies and identify molecular correlates of response versus resistance, and to be flexible enough to address individual investigator needs. In the previous funding period, the tissue core was located at the well established Mayo Clinic Myeloma Core facilities which received, processed, stored, archived and distributed clinically annotated cellular (bone marrow and PB cells) and molecular (DMA and RNA) and serum samples to investigators participating in this SPORE. Samples from the SPORE have been collected, tested, and archived separately from other Core resources, such as the ECOG or the Mayo Program Project reference laboratories; however, these resources have been shared between these cores resources, thereby increasing the pool of samples available for correlative science by the SPORE investigators. Due to the increased needs of our growing SPORE and in close cooperation and collaboration with the core at the Mayo clinic, we have now established a tissue core facility at the Dana Farber Cancer Institute. To make efforts at the Mayo Clinic and at the DFCI seamless and compatible for annotated sample sharing, we have utilized the same standard operating procedures for bone marrow processing, myeloma cell purification, and cryopreservation. Our biostatical and clinical data management at both the institutions are integrated in Core 3, which allows sharing of data and compatibility in sample selection for research studies. This Core provides an independent, current, and quickly searchable database of clinical and laboratory results and archived biospecimens. Data forms completed at the time of collection of the samples are entered into a common clinical, laboratory, and archival database. Follow up data is obtained and entered as required. All data are then stored in a common database accessible by the Statistics center. All patients have measurement of key biological variables requested by SPORE investigators. Unused specimen including sorted cells, DNA, RNA and cytospin slides are stored for future use on all patients. Subsequent use of banked samples beyond that specified in this SPORE proposal will be provided only with agreement from SPORE Director and the SPORE Principal Investigators. This Core is lead by Drs. Fonseca and Munshi, who are experienced in managing Core laboratories in a multi-institutional cooperative setting.