Role of the CLAVATA1 Receptor Kinase in Plant Stem Cell Regulation

CLAVATA1 受体激酶在植物干细胞调节中的作用

• 批准号: 7685321
• 负责人: ELLIOT M MEYEROWITZ
• 金额: $ 33.71万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-10 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7685321
• 关键词: Angiosperms; Apical; Arabidopsis; Biochemical; Cell Communication; Cells; Cleaved cell; Communication; Complex; Degenerative Disorder; Development; Disease; Embryo; Endocytosis; Flowers; Food; Future; Gene Proteins; Generations; Genes; Genetic; Genetic Screening; Genotype; Goals; Health; Human; Human Development; Image; Knowledge; Left; Leucine-Rich Repeat; Life; Ligands; Light; Location; Maintenance; Mammals; Meristem; Methods; Mouse-ear Cress; Multipotent Stem Cells; Organ; Organism; Pattern; Phosphotransferases; Plant Sources; Plant Stems; Plants; Play; Pluripotent Stem Cells; Proteins; Public Health; Receptor Serine/Threonine Kinase; Receptor Signaling; Regulation; Relative (related person); Research; Resolution; Role; Signal Transduction; Signal Transduction Pathway; Signaling Protein; Source; Stem cells; Surface; System; Testing; Time; Tissue Engineering; Work; base; cell behavior; cell type; cellular imaging; design; gene cloning; genome sequencing; homeodomain; imaging modality; mutant; novel; plant growth/development; public health relevance; receptor; receptor internalization; research study; rib bone structure; stem; stem cell population; transcription factor

DESCRIPTION (provided by applicant): The long-term objective of the proposed research is to understand the role of cell-cell communication in the maintenance and control of stem cell populations. As stem cells are of central importance in human development, and are of potential use in disease treatments, understanding how they work is an important public health goal. The organism chosen for the experiments is the flowering plant Arabidopsis thaliana, and the stem cell population the shoot apical meristem, which forms in embryos and is the source of all of the above-ground parts of the plant, including stem, leaves, and flowers. This plant has been chosen because its stem cells are particularly amenable to experiment - not only is there a great deal of background information on their patterns of gene activity, and on the mechanisms by which they communicate with each other, but also plant stem cells are at the plant surface, which allows them to be observed while alive and functioning without any invasive methods. The experiments proposed are designed to reveal the mechanism by which one set of plant stem cells communicates with another. It is known that the first set, the central zone cells at the top of the meristem, secrete a small protein that activates a receptor protein in the cells under them (the rib meristem cells). Preliminary results have revealed that the stability and cellular location of the receptor protein are determined by the small signaling protein. The proposed experiments examine the exact mechanisms by which the small signaling protein regulates the level and location of its own receptor in nearby cells. The methods to be used include live imaging of the cells and proteins involved, isolation of the genes and proteins involved, and also genetic screens for additional proteins that work along with the signal and receptor to control stem cell behavior. PUBLIC HEALTH RELEVANCE: A fundamental understanding of stem cell populations in plants will reveal basic principles of development and stem cell behavior, and will therefore eventually enable tissue engineering, and the use of genetically matched self-cells as a source of needed cell types and bodily organs to cure diseases and degenerative conditions. Plant development itself is also of direct health significance, as plants are the source of more than 80% of human food. A profound understanding of plant growth and development is a key to our ability to grow sufficient and nutritious food for the world in the future.

描述（由申请人提供）：拟议研究的长期目标是了解细胞 - 细胞通信在维持和控制干细胞种群中的作用。由于干细胞在人类发展中至关重要，并且在疾病治疗中潜在使用，因此了解它们的工作方式是重要的公共卫生目标。为实验选择的有机体是开花植物拟南芥，干细胞种群芽根基分生组织，它是在胚胎中形成的，是植物所有地上部分的来源，包括茎，叶子和花朵。之所以选择该植物，是因为其干细胞特别适合实验 - 不仅有大量背景信息有关其基因活性模式，以及它们相互交流的机制，而且植物干细胞在植物表面上也可以在植物的表面上观察到它们的生命并在没有任何侵入性方法的情况下活着并发挥作用。提出的实验旨在揭示一组植物干细胞与另一种植物干细胞通信的机制。众所周知，第一组是分生组织顶部的中央区域细胞，分泌了一种小蛋白，该蛋白会激活其下面细胞中的受体蛋白（肋骨分生组织细胞）。初步结果表明，受体蛋白的稳定性和细胞位置由小信号蛋白确定。提出的实验检查了小信号蛋白调节其自身受体在附近细胞中的水平和位置的确切机制。要使用的方法包括对所涉及的细胞和蛋白质的实时成像，所涉及的基因和蛋白质的分离，以及与信号和受体一起使用以控制干细胞行为的其他蛋白质的遗传筛选。公共卫生相关性：对植物中干细胞种群的基本理解将揭示发展和干细胞行为的基本原理，因此最终将使组织工程以及使用遗传匹配的自我细胞作为所需细胞类型的来源和身体器官来治愈疾病和变性状况。植物发育本身也具有直接的健康意义，因为植物是超过80％的人类食品的来源。对植物生长和发展的深刻了解是我们将来为世界增长足够和营养食品的能力的关键。